Validated using a knockout cell line
Recombinant
RabMAb

Recombinant Anti-Ctip1/BCL-11A antibody [EPR14943-44] - BSA and Azide free (ab242406)

Overview

  • Product name

    Anti-Ctip1/BCL-11A antibody [EPR14943-44] - BSA and Azide free
    See all Ctip1/BCL-11A primary antibodies
  • Description

    Rabbit monoclonal [EPR14943-44] to Ctip1/BCL-11A - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, IHC-P, IP, WB, Flow Cytmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment within Human Ctip1/BCL-11A aa 400-550. The exact sequence is proprietary.
    Database link: Q9H165

  • Positive control

    • WB: Raji and HAP cell lysates; IHC-P: Human tonsil and rat spleen tissues; rat C6 cells. IP: Raji whole cell lysate. Flow cyt: Jurkat cells.
  • General notes

    Ab242406 is the carrier-free version of ab191401. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab242406 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab242406 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
IP Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Detects a band of approximately 120 kDa (predicted molecular weight: 91 kDa).
Flow Cyt Use at an assay dependent concentration.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

Target

  • Function

    Functions as a myeloid and B-cell proto-oncogene. May play important roles in leukemogenesis and hematopoiesis. An essential factor in lymphopoiesis, is required for B-cell formation in fetal liver. May function as a modulator of the transcriptional repression activity of ARP1.
  • Tissue specificity

    Expressed at high levels in brain, spleen thymus, bone marrow and testis. Expressed in CD34-positive myeloid precursor cells, B-cells, monocytes and megakaryocytes. Expression is tightly regulated during B-cell development.
  • Involvement in disease

    Note=Chromosomal aberrations involving BCL11A may be a cause of lymphoid malignancies. Translocation t(2;14)(p13;q32.3) causes BCL11A deregulation and amplification.
  • Sequence similarities

    Contains 6 C2H2-type zinc fingers.
  • Post-translational
    modifications

    Sumoylated by SUMO1.
  • Cellular localization

    Cytoplasm. Nucleus. Associates with the nuclear body. Colocalizes with SUMO1 and SENP2 in nuclear speckles.
  • Information by UniProt
  • Database links

  • Alternative names

    • 2810047E18Rik antibody
    • B cell CLL/lymphoma 11A (zinc finger protein) antibody
    • B cell CLL/lymphoma 11A (zinc finger protein) isoform 2 antibody
    • B-cell CLL/lymphoma 11A antibody
    • B-cell lymphoma/leukemia 11A antibody
    • BC11A_HUMAN antibody
    • BCL-11A antibody
    • BCL11A antibody
    • BCL11A B cell CLL/lymphoma 11A (zinc finger protein) isoform 1 antibody
    • BCL11A L antibody
    • BCL11A S antibody
    • BCL11A XL antibody
    • BCL11a-M antibody
    • BCL11AL antibody
    • BCL11AS antibody
    • BCL11AXL antibody
    • C2H2 type zinc finger protein antibody
    • COUP TF interacting protein 1 antibody
    • COUP-TF-interacting protein 1 antibody
    • CTIP1 antibody
    • CTIP1, mouse, homolog of antibody
    • D930021L15Rik antibody
    • Ecotropic viral integration site 9 antibody
    • Ecotropic viral integration site 9 homolog antibody
    • Ecotropic viral integration site 9 protein antibody
    • Ecotropic viral integration site 9 protein homolog antibody
    • EVI-9 antibody
    • Evi9 antibody
    • Evi9, mouse, homolog of antibody
    • FLJ10173 antibody
    • FLJ34997 antibody
    • HBFQTL5 antibody
    • KIAA1809 antibody
    • mKIAA1809 antibody
    • OTTHUMP00000159788 antibody
    • OTTHUMP00000159789 antibody
    • OTTHUMP00000201250 antibody
    • OTTHUMP00000202084 antibody
    • Zinc finger protein 856 antibody
    • ZNF856 antibody
    see all

Images

  • Lane 1: Wild type HAP1 whole cell lysate (20 µg)
    Lane 2: Empty
    Lane 3: BCL11A (Ctip1) knockout HAP1 whole cell lysate (20 µg)
    Lane 4: Raji whole cell lysate (20 µg)

    Lanes 1 - 4: Merged signal (red and green). Green - ab191401 observed at 91 kDa. Red - loading control, ab8245, observed at 37 kDa.

    ab191401 was shown to recognize BCL11A (Ctip1) in wild type cells as signal was lost at the expected MW in BCL11A (Ctip1) knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and BCL11A (Ctip1) knockout samples were subjected to SDS-PAGE. Ab191401 and ab8245 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1000 dilution and 1/10000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab191401)

  • ab191401 (purified) at 1/500 dilution (2.1 �g/ml) immunoprecipitating Ctip1/BCL-11A in Raji whole cell lysate.
    Lane 1 (input): Raji(Human Burkitt's lymphoma B lymphocyte) whole cell lysate 10�g
    Lane 2 (+): ab191401 & Raji whole cell lysate
    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab191401 in Raji whole cell lysate
    For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1/1000 dilution.
    Blocking and diluting buffer: 5% NFDM /TBST .

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab191401)

  • Flow Cytometry analysis of Jurkat (human acute T cell leukemia) labelling Ctip1/BCL-11A with purified ab191401 at 1/200 (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Alexa Fluor® 488 goat anti-rabbit IgG (1/2000) was used as the secondary antibody. Black - Isotype control, rabbit monoclonal IgG. Blue - Unlabelled control, cells without incubation with primary and secondary antibodies.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab191401)

  • Immunohistochemical analysis of paraffin-embedded, Human tonsil tissue labeling Ctip1/BCL-11A with ab191401 at a 1/100 dilution (19 μg/ml). Counter stained with hematoxylin. In the negative control PBS was used instead of primary antibody.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab191401)

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemical analysis of paraffin-embedded, rat spleen tissue labeling Ctip1/BCL-11A with ab191401 at a 1/100 dilution (19 μg/ml). Counter stained with hematoxylin. In the negative control PBS was used instead of primary antibody.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab191401)

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunofluorescence analysis of, paraformaldehyde-fixed, rat C6 cells labeling Ctip1/BCL-11A with ab191401 at a 1/450 dilution (4 ug/ml). As secondary antibody goat anti-rabbit IgG (Alexa Fluor®488) ab150077 was used at a 1/200 dilution. In blue DAPI staining. In the negative controls cells were treated with anti-BCL11A at a 1/450 dilution as primary antibody and goat anti-mouse IgG (Alexa Fluor®594) at a 1/400 dilution as secondary antibody.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab191401)

References

ab242406 has not yet been referenced specifically in any publications.

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