Anti-Ctip2 antibody [1F8G8] (ab233713)
Key features and details
- Mouse monoclonal [1F8G8] to Ctip2
- Suitable for: WB, IHC-P, Flow Cyt, ICC/IF
- Reacts with: Human
- Isotype: IgG1
Overview
-
Product name
Anti-Ctip2 antibody [1F8G8]
See all Ctip2 primary antibodies -
Description
Mouse monoclonal [1F8G8] to Ctip2 -
Host species
Mouse -
Tested applications
Suitable for: WB, IHC-P, Flow Cyt, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment corresponding to Human Ctip2 aa 1-150. (Expressed in E.coli).
Database link: Q9C0K0 -
Positive control
- WB: Recombinant human Ctip2 (aa1-150) protein; Ctip2 (aa1-150)-hIgG-Fc-transfected HEK-293 cell lysate. ICC/IF: HeLa cells. Flow cyt: HeLa and Jurkat cells. IHC-P: Human colon cancer and stomach cancer tissues.
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituent: PBS -
Concentration information loading...
-
Purity
Protein G purified -
Purification notes
Purified from tissue culture supernatant. -
Clonality
Monoclonal -
Clone number
1F8G8 -
Isotype
IgG1 -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab233713 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
1/500 - 1/2000. Predicted molecular weight: 96 kDa.
|
|
IHC-P |
1/200 - 1/1000.
|
|
Flow Cyt |
1/200 - 1/400.
|
|
ICC/IF |
1/50 - 1/250.
|
Notes |
---|
WB
1/500 - 1/2000. Predicted molecular weight: 96 kDa. |
IHC-P
1/200 - 1/1000. |
Flow Cyt
1/200 - 1/400. |
ICC/IF
1/50 - 1/250. |
Target
-
Function
Tumor-suppressor protein involved in T-cell lymphomas. May function on the P53-signaling pathway. May be a key regulator of both differentiation and survival during thymocyte development. Repress transcription through direct, TFCOUP2-independent binding to a GC-rich response element. -
Tissue specificity
Highly expressed in brain and in malignant T-cell lines derived from patients with adult T-cell leukemia/lymphoma. -
Sequence similarities
Contains 6 C2H2-type zinc fingers. -
Cellular localization
Nucleus. - Information by UniProt
-
Database links
- Entrez Gene: 64919 Human
- Omim: 606558 Human
- SwissProt: Q9C0K0 Human
- Unigene: 709690 Human
-
Alternative names
- ATL1 alpha antibody
- ATL1 antibody
- ATL1 beta antibody
see all
Images
-
Paraffin-embedded human colon cancer tissue stained for Ctip2 using ab233713 at 1/200 dilution in immunohistochemical analysis. DAB staining.
-
Anti-Ctip2 antibody [1F8G8] (ab233713) at 1/500 dilution + Recombinant human Ctip2 (aa1-150) protein
Predicted band size: 96 kDa -
All lanes : Anti-Ctip2 antibody [1F8G8] (ab233713) at 1/500 dilution
Lane 1 : Non-transfected HEK-293 (human epithelial cell line from embryonic kidney) cell lysate
Lane 2 : Ctip2 (aa1-150)-hIgG-Fc-transfected HEK-293 cell lysate
Predicted band size: 96 kDa -
Flow cytometric analysis of HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling Ctip2 with ab233713 at 1/200 dilution (green) compared with a negative control (red).
-
Flow cytometric analysis of Jurkat (human T cell leukemia cell line from peripheral blood) cells labeling Ctip2 with ab233713 at 1/200 dilution (green) compared with a negative control (red).
-
HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for Ctip2 (green) using ab233713 at 1/50 dilution in ICC/IF. Actin is stained with AlexaFluor®555-Phalloidin (red). Blue: DRAQ5 DNA dye.
-
Paraffin-embedded human stomach cancer tissue stained for Ctip2 using ab233713 at 1/200 dilution in immunohistochemical analysis. DAB staining.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
-
Datasheet download
References (0)
ab233713 has not yet been referenced specifically in any publications.