Overview

  • Product name

    Anti-Ctip2 antibody [1F8G8]
    See all Ctip2 primary antibodies
  • Description

    Mouse monoclonal [1F8G8] to Ctip2
  • Host species

    Mouse
  • Tested applications

    Suitable for: WB, IHC-P, Flow Cyt, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment corresponding to Human Ctip2 aa 1-150. (Expressed in E.coli).
    Database link: Q9C0K0

  • Positive control

    • WB: Recombinant human Ctip2 (aa1-150) protein; Ctip2 (aa1-150)-hIgG-Fc-transfected HEK-293 cell lysate. ICC/IF: HeLa cells. Flow cyt: HeLa and Jurkat cells. IHC-P: Human colon cancer and stomach cancer tissues.

Properties

Applications

Our Abpromise guarantee covers the use of ab233713 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/2000. Predicted molecular weight: 96 kDa.
IHC-P 1/200 - 1/1000.
Flow Cyt 1/200 - 1/400.
ICC/IF 1/50 - 1/250.

Target

  • Function

    Tumor-suppressor protein involved in T-cell lymphomas. May function on the P53-signaling pathway. May be a key regulator of both differentiation and survival during thymocyte development. Repress transcription through direct, TFCOUP2-independent binding to a GC-rich response element.
  • Tissue specificity

    Highly expressed in brain and in malignant T-cell lines derived from patients with adult T-cell leukemia/lymphoma.
  • Sequence similarities

    Contains 6 C2H2-type zinc fingers.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • ATL1 alpha antibody
    • ATL1 antibody
    • ATL1 beta antibody
    • ATL1 gamma antibody
    • ATL1-delta antibody
    • B cell CLL/lymphoma 11B/T cell receptor delta constant region fusion protein antibody
    • B cell lymphoma/leukemia 11B antibody
    • B-cell CLL/lymphoma 11B (zinc finger protein) antibody
    • B-cell CLL/lymphoma 11B antibody
    • B-cell lymphoma/leukemia 11B antibody
    • BC11B_HUMAN antibody
    • BCL-11B antibody
    • Bcl11b antibody
    • BCL11B/TRDC fusion antibody
    • COUP TF interacting protein 2 antibody
    • COUP-TF interacting protein 2 antibody
    • COUP-TF-interacting protein 2 antibody
    • Ctip 2 antibody
    • CTIP-2 antibody
    • CTIP2 antibody
    • hRIT1 alpha antibody
    • hRit1 antibody
    • Radiation induced tumor suppressor gene 1 antibody
    • Radiation induced tumor suppressor gene 1 protein antibody
    • Radiation-induced tumor suppressor gene 1 protein antibody
    • Rit 1 antibody
    • Rit1 antibody
    • zinc finger protein hRit1 alpha antibody
    • ZNF856B antibody
    see all

Images

  • Paraffin-embedded human colon cancer tissue stained for Ctip2 using ab233713 at 1/200 dilution in immunohistochemical analysis. DAB staining.

  • Anti-Ctip2 antibody [1F8G8] (ab233713) at 1/500 dilution + Recombinant human Ctip2 (aa1-150) protein

    Predicted band size: 96 kDa

  • All lanes : Anti-Ctip2 antibody [1F8G8] (ab233713) at 1/500 dilution

    Lane 1 : Non-transfected HEK-293 (human epithelial cell line from embryonic kidney) cell lysate
    Lane 2 : Ctip2 (aa1-150)-hIgG-Fc-transfected HEK-293 cell lysate

    Predicted band size: 96 kDa

  • Flow cytometric analysis of HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling Ctip2 with ab233713 at 1/200 dilution (green) compared with a negative control (red).

  • Flow cytometric analysis of Jurkat (human T cell leukemia cell line from peripheral blood) cells labeling Ctip2 with ab233713 at 1/200 dilution (green) compared with a negative control (red).

  • HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for Ctip2 (green) using ab233713 at 1/50 dilution in ICC/IF. Actin is stained with AlexaFluor®555-Phalloidin (red). Blue: DRAQ5 DNA dye.

  • Paraffin-embedded human stomach cancer tissue stained for Ctip2 using ab233713 at 1/200 dilution in immunohistochemical analysis. DAB staining.

References

ab233713 has not yet been referenced specifically in any publications.

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