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  1. Link

    ctip2-antibody-25b6-ab18465.pdf

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Epigenetics and Nuclear Signaling Chromatin Modifying Enzymes Acetylation
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Anti-Ctip2 antibody [25B6] (ab18465)

  • Datasheet
  • SDS
Reviews (36)Q&A (17)References (567)

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Immunocytochemistry/ Immunofluorescence - Anti-Ctip2 antibody [25B6] - ChIP Grade (ab18465)
  • Western blot - Anti-Ctip2 antibody [25B6] - ChIP Grade (ab18465)
  • Immunocytochemistry/ Immunofluorescence - Anti-Ctip2 antibody [25B6] - ChIP Grade (ab18465)
  • Flow Cytometry - Anti-Ctip2 antibody [25B6] - ChIP Grade (ab18465)
  • Western blot - Anti-Ctip2 antibody [25B6] - ChIP Grade (ab18465)

Key features and details

  • Rat monoclonal [25B6] to Ctip2
  • Suitable for: ICC/IF, WB, Flow Cyt
  • Reacts with: Mouse, Human
  • Isotype: IgG2a

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Overview

  • Product name

    Anti-Ctip2 antibody [25B6]
    See all Ctip2 primary antibodies
  • Description

    Rat monoclonal [25B6] to Ctip2
  • Host species

    Rat
  • Specificity

    Detects 2 bands representing Ctip2 at about 120kD. Ctip2 is highly expressed in brain and in malignant T-cell lines derived from patients with adult T-cell leukemia/lymphoma.
  • Tested applications

    Suitable for: ICC/IF, WB, Flow Cytmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Recombinant fragment corresponding to Human Ctip2. GTS fusion
    Database link: Q9C0K0

  • Epitope

    Between amino acids 1-150 of CTIP2.
  • Positive control

    • Flow Cyt: Jurkat cells. ICC: Neonatal mouse hippocampal cultured neurons WB: Nuclear extract from Jurkat cells; Mouse brain tissue lysate.
  • General notes

    Hybridoma produced by fusion of a rat lymphocyte and mouse myeloma.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.50
    Preservative: 0.02% Sodium azide
    Constituents: 0.357% HEPES, 0.87% Sodium chloride
  • Concentration information loading...
  • Purity

    Multi-step Chromatography
  • Clonality

    Monoclonal
  • Clone number

    25B6
  • Isotype

    IgG2a
  • Research areas

    • Epigenetics and Nuclear Signaling
    • Chromatin Modifying Enzymes
    • Acetylation
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Domain Families
    • Zinc Finger
    • Neuroscience
    • Neurology process
    • Neural Signal Transduction
    • Neuroscience
    • Neurology process
    • Neurogenesis
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Transcription Factors
    • Epigenetics and Nuclear Signaling
    • Chromatin Modifying Enzymes
    • Acetylation
    • HAT
    • Neuroscience
    • Development

Associated products

  • Alternative Versions

    • FITC Anti-Ctip2 antibody [25B6] (ab123449)
  • ChIP Related Products

    • ChIP Kit (ab500)
  • Compatible Secondaries

    • Goat Anti-Rat IgG H&L (Alexa Fluor® 488) (ab150157)
    • Goat Anti-Rat IgG H&L (HRP) (ab205720)
  • Conjugation kits

    • PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918)
    • APC Conjugation Kit - Lightning-Link® (ab201807)
  • Isotype control

    • Rat IgG2a, kappa monoclonal [RTK2758] - Isotype Control (ab18450)
  • Related Products

    • Prestained Protein Ladder – Broad molecular weight (10-245 kDa) (ab116028)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab18465 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF (10)
1/500.
WB (3)
Use at an assay dependent concentration. Detects a band of approximately 120 kDa (predicted molecular weight: 95 kDa).
Flow Cyt (1)
Use 1µg for 106 cells.

ab18450 - Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

Notes
ICC/IF
1/500.
WB
Use at an assay dependent concentration. Detects a band of approximately 120 kDa (predicted molecular weight: 95 kDa).
Flow Cyt
Use 1µg for 106 cells.

ab18450 - Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

Target

  • Function

    Tumor-suppressor protein involved in T-cell lymphomas. May function on the P53-signaling pathway. May be a key regulator of both differentiation and survival during thymocyte development. Repress transcription through direct, TFCOUP2-independent binding to a GC-rich response element.
  • Tissue specificity

    Highly expressed in brain and in malignant T-cell lines derived from patients with adult T-cell leukemia/lymphoma.
  • Sequence similarities

    Contains 6 C2H2-type zinc fingers.
  • Cellular localization

    Nucleus.
  • Target information above from: UniProt accession Q9C0K0 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 64919 Human
    • Entrez Gene: 58208 Mouse
    • Omim: 606558 Human
    • SwissProt: Q9C0K0 Human
    • SwissProt: Q99PV8 Mouse
    • Unigene: 709690 Human
    • Unigene: 392694 Mouse
    • Alternative names

      • ATL1 alpha antibody
      • ATL1 antibody
      • ATL1 beta antibody
      • ATL1 gamma antibody
      • ATL1-delta antibody
      • B cell CLL/lymphoma 11B/T cell receptor delta constant region fusion protein antibody
      • B cell lymphoma/leukemia 11B antibody
      • B-cell CLL/lymphoma 11B (zinc finger protein) antibody
      • B-cell CLL/lymphoma 11B antibody
      • B-cell lymphoma/leukemia 11B antibody
      • BC11B_HUMAN antibody
      • BCL-11B antibody
      • Bcl11b antibody
      • BCL11B/TRDC fusion antibody
      • COUP TF interacting protein 2 antibody
      • COUP-TF interacting protein 2 antibody
      • COUP-TF-interacting protein 2 antibody
      • Ctip 2 antibody
      • CTIP-2 antibody
      • CTIP2 antibody
      • hRIT1 alpha antibody
      • hRit1 antibody
      • Radiation induced tumor suppressor gene 1 antibody
      • Radiation induced tumor suppressor gene 1 protein antibody
      • Radiation-induced tumor suppressor gene 1 protein antibody
      • Rit 1 antibody
      • Rit1 antibody
      • zinc finger protein hRit1 alpha antibody
      • ZNF856B antibody
      see all

    Images

    • Immunocytochemistry/ Immunofluorescence - Anti-Ctip2 antibody [25B6] - ChIP Grade (ab18465)
      Immunocytochemistry/ Immunofluorescence - Anti-Ctip2 antibody [25B6] - ChIP Grade (ab18465)
      Neonatal mouse hippocampal neurons stained with ab18465 (top panel - green) and Ctip1 antibody (middle - red). Bottom panel is overlay of ab18465 and Ctip1 antibody staining - yellow indicates co-localisation, green is ab18465 alone and red is Ctip1 antibody alone.
    • Western blot - Anti-Ctip2 antibody [25B6] - ChIP Grade (ab18465)
      Western blot - Anti-Ctip2 antibody [25B6] - ChIP Grade (ab18465)

      Western blot using ab18465 on nuclear extract from Jurkat cells immunoprecipitated with anti-Sir2 antibody.

      Two bands are seen which may correspond to two CTIP2 transcripts present in Jurkat cells as previously reported (Bernard et al. 2001).

      Western blot using ab18465 on nuclear extract from Jurkat cells immunoprecipitated with anti-Sir2 antibody. Two bands are seen which may correspond to two CTIP2 transcripts present in Jurkat cells as previously reported (Bernard et al. 2001).
    • Immunocytochemistry/ Immunofluorescence - Anti-Ctip2 antibody [25B6] - ChIP Grade (ab18465)
      Immunocytochemistry/ Immunofluorescence - Anti-Ctip2 antibody [25B6] - ChIP Grade (ab18465)

      Neonatal Mouse Hippocampal Neurons (Harvested at P1, grown 5d in culture on glial cell feeder layer).

      Red is beta tubulin staining.
      Green is ab18465.

    • Flow Cytometry - Anti-Ctip2 antibody [25B6] - ChIP Grade (ab18465)
      Flow Cytometry - Anti-Ctip2 antibody [25B6] - ChIP Grade (ab18465)

      Overlay histogram showing Jurkat cells stained with ab18465 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab18465, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rat IgG (H+L) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rat IgG (2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

    • Western blot - Anti-Ctip2 antibody [25B6] - ChIP Grade (ab18465)
      Western blot - Anti-Ctip2 antibody [25B6] - ChIP Grade (ab18465)This image is courtesy of an Anonymous Abreview.
      All lanes : Anti-Ctip2 antibody [25B6] (ab18465) at 1/500 dilution

      Lanes 1-2 : Mouse brain tissue lysate at 1.5 µg
      Lane 3 : Mouse brain tissue lysate at 3 µg

      Secondary
      All lanes : IRDYE 680-conjugated Donkey Anti-Rat polyclonal. at 1/10000 dilution

      Performed under reducing conditions.

      Predicted band size: 95 kDa
      Observed band size: 100,110 kDa why is the actual band size different from the predicted?


      Exposure time: 10 minutes

      See Abreview

    Protocols

    • Flow cytometry protocols
    • Immunocytochemistry & immunofluorescence protocols
    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (567)

    Publishing research using ab18465? Please let us know so that we can cite the reference in this datasheet.

    ab18465 has been referenced in 567 publications.

    • Dang J  et al. Glial cell diversity and methamphetamine-induced neuroinflammation in human cerebral organoids. Mol Psychiatry 26:1194-1207 (2021). PubMed: 32051547
    • Harlan De Crescenzo A  et al. Deficient or Excess Folic Acid Supply During Pregnancy Alter Cortical Neurodevelopment in Mouse Offspring. Cereb Cortex 31:635-649 (2021). PubMed: 32995858
    • Murillo A  et al. Temporal Dynamics and Neuronal Specificity of Grin3a Expression in the Mouse Forebrain. Cereb Cortex 31:1914-1926 (2021). PubMed: 33290502
    • Canales CP  et al. Sequential perturbations to mouse corticogenesis following in utero maternal immune activation. Elife 10:N/A (2021). PubMed: 33666173
    • Kement D  et al. Neuroserpin Is Strongly Expressed in the Developing and Adult Mouse Neocortex but Its Absence Does Not Perturb Cortical Lamination and Synaptic Proteome. Front Neuroanat 15:627896 (2021). PubMed: 33708076
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-10 of 53 Abreviews or Q&A

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Ctip2 antibody [25B6]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (iPSC-derived neuron)
    Permeabilization
    Yes - Triton X-100
    Specification
    iPSC-derived neuron
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Nov 22 2021

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Ctip2 antibody [25B6] - ChIP Grade

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (iPSC-derived cortical neurons)
    Permeabilization
    Yes - 0.3% Triton X-100
    Specification
    iPSC-derived cortical neurons
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 23°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Dec 26 2019

    Immunohistochemistry (Frozen sections) abreview for Anti-Ctip2 antibody [25B6] - ChIP Grade

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Human Tissue sections (Cerebral organoid)
    Permeabilization
    Yes - 0.25% Triton-X 100
    Specification
    Cerebral organoid
    Blocking step
    Serum as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 4% · Temperature: 25°C
    Fixative
    Paraformaldehyde
    Read More

    Dr. Iva Kelava

    Verified customer

    Submitted Dec 20 2019

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Ctip2 antibody [25B6] - ChIP Grade

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (brain)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Vetor Unmasking solution
    Permeabilization
    Yes - 0.05% triton-x100
    Specification
    brain
    Blocking step
    Vetor Blocker as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 23°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Nov 15 2019

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Ctip2 antibody [25B6] - ChIP Grade

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Monkey Tissue sections (Marmoset spleen)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citric acid
    Permeabilization
    No
    Specification
    Marmoset spleen
    Blocking step
    BSA as blocking agent for 10 minute(s) · Concentration: 2% · Temperature: 21°C
    Fixative
    Formaldehyde
    Read More

    MR. Carl Hobbs

    Verified customer

    Submitted May 31 2018

    Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-Ctip2 antibody [25B6] - ChIP Grade

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (PFA perfusion fixed frozen sections)
    Sample
    Mouse Tissue sections (e16.5 Brain)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citric acid
    Permeabilization
    No
    Specification
    e16.5 Brain
    Blocking step
    BSA as blocking agent for 10 minute(s) · Concentration: 2% · Temperature: 21°C
    Fixative
    Formaldehyde
    Read More

    MR. Carl Hobbs

    Verified customer

    Submitted Apr 26 2018

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Ctip2 antibody [25B6] - ChIP Grade

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Rat Tissue sections (Brain)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citric acid
    Permeabilization
    No
    Specification
    Brain
    Blocking step
    BSA as blocking agent for 10 minute(s) · Concentration: 2% · Temperature: 21°C
    Fixative
    Formaldehyde
    Read More

    MR. Carl Hobbs

    Verified customer

    Submitted Apr 04 2018

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Ctip2 antibody [25B6] - ChIP Grade

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (Tonsil)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citric acid
    Permeabilization
    No
    Specification
    Tonsil
    Blocking step
    BSA as blocking agent for 10 minute(s) · Concentration: 2% · Temperature: 21°C
    Fixative
    Formaldehyde
    Read More

    MR. Carl Hobbs

    Verified customer

    Submitted Apr 04 2018

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Ctip2 antibody [25B6] - ChIP Grade

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (e15 brain)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citric acid
    Permeabilization
    No
    Specification
    e15 brain
    Blocking step
    BSA as blocking agent for 10 minute(s) · Concentration: 2% · Temperature: 21°C
    Fixative
    Formaldehyde
    Read More

    MR. Carl Hobbs

    Verified customer

    Submitted Apr 04 2018

    Question

    fix 4%PFA 20 min. wash 3x pbs
    block: 3% NGS/0.3% triton/PBS 1 hour RT
    primary 1:400 in block 4C overnight
    wash 3x PBS
    secondary: 1:500 or 1:1000 goat anti-rat alexa 546 1 hr RT
    3x PBS wash

    Read More

    Abcam community

    Verified customer

    Asked on Apr 24 2012

    Answer

    Thanks for passing along the protocol details. They all seem quite reasonable.

    If I could I'd just like to ask a few additional questions:

    1. Are you still using same vial of antibody?

    2. Have you stored the antibody in aliquots and if so are you using the same or different aliquot now than in the past?

    3. Since your samples are dervied from stem cells, have you been able to confirm that your current cells have a neuronal phenotype?

    Thanks again for the additional details!

    Read More

    Abcam Scientific Support

    Answered on Apr 24 2012

    1-10 of 53 Abreviews or Q&A

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