Anti-Ctip2 antibody [25B6] (ab18465)

Patterns of staining in the frontal cerebral cortex of pups receiving the mitotic marker EDU on either E12 (A) or E16 (B)

Note the age-related differences in EDU (red) staining: at E12 EDU is found primarily deep in the cortex (bottom of panel), while at E16 it is found in superficial layers (top). In the E12 tissue (A) substantial numbers of cells expressing the deep marker CTIP2 (blue) co-labelled with EDU (pink) while only a few of the cells labelled with CUX also have the maker (yellow). E16 injections (B) labeled cells in layer 3 expressing the superficial marker CART as wells as more superficial cells. The marker labels all proliferating cells, including glial and perivascular cells. Scale bar = 200μm.

Ctip2 is labelled with ab18465.

(From Figure 7 of Brunjes et al)

Western blot using ab18465 on nuclear extract from Jurkat cells immunoprecipitated with anti-Sir2 antibody.

Two bands are seen which may correspond to two CTIP2 transcripts present in Jurkat cells as previously reported (Bernard et al. 2001).

Neonatal Mouse Hippocampal Neurons (Harvested at P1, grown 5d in culture on glial cell feeder layer).

Red is beta tubulin staining.
Green is ab18465.

Overlay histogram showing Jurkat cells stained with ab18465 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab18465, 1µg/1x10⁶ cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rat IgG (H+L) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rat IgG (2µg/1x10⁶ cells) used under the same conditions. Acquisition of >5,000 events was performed.

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