Anti-Ctip2 antibody (ab28448)
- Datasheet
- References (23)
- Protocols
Overview
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Product nameAnti-Ctip2 antibody
See all Ctip2 primary antibodies -
DescriptionRabbit polyclonal to Ctip2
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Host speciesRabbit
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Tested applicationsSuitable for: IP, WB, IHC-FoFr, IHC-Frmore details
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Species reactivityReacts with: Mouse, Rat, Human
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Immunogen
Synthetic peptide conjugated to KLH derived from within residues 850 - 950 of Human Ctip2.
Read Abcam's proprietary immunogen policy (Peptides available as ab32404) -
Positive control
- This antibody gave a positive signal in the following human cell lysates: Jurkat; Jurkat Nuclear
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4 -
Concentration information loading... -
PurityImmunogen affinity purified
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab28448 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IP | Use at an assay dependent concentration. | |
| WB | Use a concentration of 1 µg/ml. Detects a band of approximately 125,130 kDa (predicted molecular weight: 96 kDa). | |
| IHC-FoFr | 1/300. | |
| IHC-Fr | 1/1000. |
Target
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FunctionTumor-suppressor protein involved in T-cell lymphomas. May function on the P53-signaling pathway. May be a key regulator of both differentiation and survival during thymocyte development. Repress transcription through direct, TFCOUP2-independent binding to a GC-rich response element.
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Tissue specificityHighly expressed in brain and in malignant T-cell lines derived from patients with adult T-cell leukemia/lymphoma.
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Sequence similaritiesContains 6 C2H2-type zinc fingers.
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Cellular localizationNucleus.
- Information by UniProt
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Database links
- Entrez Gene: 64919 Human
- Entrez Gene: 58208 Mouse
- Entrez Gene: 314423 Rat
- Omim: 606558 Human
- SwissProt: Q9C0K0 Human
- SwissProt: Q99PV8 Mouse
- Unigene: 709690 Human
- Unigene: 392694 Mouse
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Alternative names
- ATL1 alpha antibody
- ATL1 antibody
- ATL1 beta antibody
see all
Images
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Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-Ctip2 antibody (ab28448)This image is courtesy of an abreview submitted by Sophie Pezet, Laboratoire de Neurobiologie, FranceIHC-FoFr image of Ctip2 staining on rat hippocampus sections using ab28448 (1:300). The sections used came from animals perfused fixed with Paraformaldehyde 4% with 15% of a solution of saturated picric acid, in phosphate buffer 0.1M. Following postfixation in the same fixative overnight, the brains were cryoprotected in sucrose 30% overnight. Brains were then cut using a cryostat and the immunostainings were performed using the ‘free floating’ technique. -
Western blot - Anti-Ctip2 antibody (ab28448)All lanes : Anti-Ctip2 antibody (ab28448) at 1 µg/ml
Lane 1 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lane 2 :Jurkat nuclear extract lysate (ab14844)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 96 kDa
Observed band size: 125,130 kDa why is the actual band size different from the predicted? -
Immunoprecipitation - Anti-Ctip2 antibody (ab28448)Ctip2 was immunoprecipitated using 0.5mg Jurkat whole cell extract, 5µg of Rabbit polyclonal to Ctip2 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
The antibody was incubated under agitation with Protein G beads for 10min, Jurkat whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab28448.
Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).
Bands: 125, 130kDa: Ctip2.
Datasheets and documents
References
This product has been referenced in:
- Wang CY et al. Function of B-Cell CLL/Lymphoma 11B in Glial Progenitor Proliferation and Oligodendrocyte Maturation. Front Mol Neurosci 11:4 (2018). IHC ; Rat . Read more (PubMed: 29416501) »
- Kelly SM et al. Radial Glial Lineage Progression and Differential Intermediate Progenitor Amplification Underlie Striatal Compartments and Circuit Organization. Neuron 99:345-361.e4 (2018). Read more (PubMed: 30017396) »
