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  1. Link

    ctip2-antibody-ab28448.pdf

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Epigenetics and Nuclear Signaling Chromatin Modifying Enzymes Acetylation
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Anti-Ctip2 antibody (ab28448)

  • Datasheet
Reviews (5) Submit a question References (37)

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Western blot - Anti-Ctip2 antibody (ab28448)
  • Immunoprecipitation - Anti-Ctip2 antibody (ab28448)

Key features and details

  • Rabbit polyclonal to Ctip2
  • Suitable for: IP, WB
  • Reacts with: Human
  • Isotype: IgG

Get better batch-to-batch reproducibility with a recombinant antibody

Product image
Anti-Ctip2 antibody [EPR23120-25] (ab240636)
  • Research with confidence – consistent and reproducible results with every batch
  • Long-term and scalable supply – powered by recombinant technology for fast production
  • Success from the first experiment – confirmed specificity through extensive validation
  • Ethical standards compliant – production is animal-free

Overview

  • Product name

    Anti-Ctip2 antibody
    See all Ctip2 primary antibodies
  • Description

    Rabbit polyclonal to Ctip2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IP, WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 850 - 950 of Human Ctip2.

    Read Abcam's proprietary immunogen policy (Peptides available as ab32404)
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS

    Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • Chromatin Modifying Enzymes
    • Acetylation
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Domain Families
    • Zinc Finger
    • Neuroscience
    • Neurology process
    • Neural Signal Transduction
    • Neuroscience
    • Neurology process
    • Neurogenesis
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Transcription Factors
    • Epigenetics and Nuclear Signaling
    • Chromatin Modifying Enzymes
    • Acetylation
    • HAT
    • Neuroscience
    • Development

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab28448 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP
Use at an assay dependent concentration.
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 125,130 kDa (predicted molecular weight: 96 kDa).
Notes
IP
Use at an assay dependent concentration.
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 125,130 kDa (predicted molecular weight: 96 kDa).

Target

  • Function

    Tumor-suppressor protein involved in T-cell lymphomas. May function on the P53-signaling pathway. May be a key regulator of both differentiation and survival during thymocyte development. Repress transcription through direct, TFCOUP2-independent binding to a GC-rich response element.
  • Tissue specificity

    Highly expressed in brain and in malignant T-cell lines derived from patients with adult T-cell leukemia/lymphoma.
  • Sequence similarities

    Contains 6 C2H2-type zinc fingers.
  • Cellular localization

    Nucleus.
  • Target information above from: UniProt accession Q9C0K0 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 64919 Human
    • Entrez Gene: 58208 Mouse
    • Entrez Gene: 314423 Rat
    • Omim: 606558 Human
    • SwissProt: Q9C0K0 Human
    • SwissProt: Q99PV8 Mouse
    • Unigene: 709690 Human
    • Unigene: 392694 Mouse
    • Alternative names

      • ATL1 alpha antibody
      • ATL1 antibody
      • ATL1 beta antibody
      • ATL1 gamma antibody
      • ATL1-delta antibody
      • B cell CLL/lymphoma 11B/T cell receptor delta constant region fusion protein antibody
      • B cell lymphoma/leukemia 11B antibody
      • B-cell CLL/lymphoma 11B (zinc finger protein) antibody
      • B-cell CLL/lymphoma 11B antibody
      • B-cell lymphoma/leukemia 11B antibody
      • BC11B_HUMAN antibody
      • BCL-11B antibody
      • Bcl11b antibody
      • BCL11B/TRDC fusion antibody
      • COUP TF interacting protein 2 antibody
      • COUP-TF interacting protein 2 antibody
      • COUP-TF-interacting protein 2 antibody
      • Ctip 2 antibody
      • CTIP-2 antibody
      • CTIP2 antibody
      • hRIT1 alpha antibody
      • hRit1 antibody
      • Radiation induced tumor suppressor gene 1 antibody
      • Radiation induced tumor suppressor gene 1 protein antibody
      • Radiation-induced tumor suppressor gene 1 protein antibody
      • Rit 1 antibody
      • Rit1 antibody
      • zinc finger protein hRit1 alpha antibody
      • ZNF856B antibody
      see all

    Images

    • Western blot - Anti-Ctip2 antibody (ab28448)
      Western blot - Anti-Ctip2 antibody (ab28448)
      All lanes : Anti-Ctip2 antibody (ab28448) at 1 µg/ml

      Lane 1 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
      Lane 2 : Jurkat nuclear extract lysate (ab14844)

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

      Performed under reducing conditions.

      Predicted band size: 96 kDa
      Observed band size: 125,130 kDa why is the actual band size different from the predicted?

    • Immunoprecipitation - Anti-Ctip2 antibody (ab28448)
      Immunoprecipitation - Anti-Ctip2 antibody (ab28448)
      Ctip2 was immunoprecipitated using 0.5mg Jurkat whole cell extract, 5µg of Rabbit polyclonal to Ctip2 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
      The antibody was incubated under agitation with Protein G beads for 10min, Jurkat whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
      Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab28448.
      Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).
      Bands: 125, 130kDa: Ctip2.

    Protocols

    • Immunoprecipitation protocols
    • Immunohistochemistry protocols
    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet download

      Download

    References (37)

    Publishing research using ab28448? Please let us know so that we can cite the reference in this datasheet.

    ab28448 has been referenced in 37 publications.

    • Hu HB  et al. LPA signaling acts as a cell-extrinsic mechanism to initiate cilia disassembly and promote neurogenesis. Nat Commun 12:662 (2021). PubMed: 33510165
    • Wang L  et al. Loss of NARS1 impairs progenitor proliferation in cortical brain organoids and leads to microcephaly. Nat Commun 11:4038 (2020). PubMed: 32788587
    • Saxena M  et al. Correction: Perturbation of canonical and non-canonical BMP signaling affects migration, polarity and dendritogenesis of mouse cortical neurons (doi:10.1242/dev.147157). Development 147:N/A (2020). PubMed: 31974284
    • Khademullah CS  et al. Cortical interneuron-mediated inhibition delays the onset of amyotrophic lateral sclerosis. Brain 143:800-810 (2020). PubMed: 32203578
    • Huang R  et al. NCAM regulates temporal specification of neural progenitor cells via profilin2 during corticogenesis. J Cell Biol 219:N/A (2020). PubMed: 31816056
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-5 of 5 Abreviews or Q&A

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Ctip2 antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Mouse Cell (mouse cortical culture)
    Permeabilization
    Yes - 0.2% TritonX-100 in PBS for 3hours along with blocking solution.
    Specification
    mouse cortical culture
    Blocking step
    Serum as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 28°C
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    MR. Lenin Veeraval

    Verified customer

    Submitted Nov 06 2020

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Ctip2 antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (mouse brain)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Sodium citrate buffer
    Permeabilization
    Yes - 0.2% TritonX-100 in PBS for 3hours along with blocking solution.
    Specification
    mouse brain
    Blocking step
    Serum as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 28°C
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Nov 06 2020

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Ctip2 antibody

    Average
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (Head and Neck Squamous Cell Carcinoma)
    Specification
    Head and Neck Squamous Cell Carcinoma
    Fixative
    Paraformaldehyde
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: citrate
    Permeabilization
    Yes - PBST
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Oct 05 2012

    Immunohistochemistry (Frozen sections) abreview for Anti-Ctip2 antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Rat Tissue sections (Brain (Hippocampus))
    Specification
    Brain (Hippocampus)
    Fixative
    Paraformaldehyde
    Permeabilization
    No
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Jan 15 2009

    Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-Ctip2 antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (PFA perfusion fixed frozen sections)
    Sample
    Rat Tissue sections (brain sections)
    Specification
    brain sections
    Fixative
    Paraformaldehyde
    Antigen retrieval step
    None
    Permeabilization
    No
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    DR. Sophie Pezet

    Verified customer

    Submitted Aug 25 2008

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