Anti-Ctip2 antibody (ab70452)
Key features and details
- Rabbit polyclonal to Ctip2
- Suitable for: IHC-P, WB, IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Ctip2 antibody
See all Ctip2 primary antibodies -
Description
Rabbit polyclonal to Ctip2 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, IPmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Cow, Pig, Chimpanzee, Baboon, Rhesus monkey, Gorilla, Orangutan, Bat
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Immunogen
Synthetic peptide corresponding to a region between residues 500 and 550 of human Ctip2 (Swiss-Prot Q9C0K0).
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Positive control
- Nuclear extract from Jurkat cells
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS -
Concentration information loading... -
Purity
Affinity purified -
Purification notes
ab70452 was affinity purified using an epitope specific to Ctip2 immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab70452 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | 1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. | |
| WB | 1/5000 - 1/15000. Detects a band of approximately 120 kDa (predicted molecular weight: 99 kDa). | |
| IP | Use at 1-5 µg/mg of lysate. |
Target
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Function
Tumor-suppressor protein involved in T-cell lymphomas. May function on the P53-signaling pathway. May be a key regulator of both differentiation and survival during thymocyte development. Repress transcription through direct, TFCOUP2-independent binding to a GC-rich response element. -
Tissue specificity
Highly expressed in brain and in malignant T-cell lines derived from patients with adult T-cell leukemia/lymphoma. -
Sequence similarities
Contains 6 C2H2-type zinc fingers. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 64919 Human
- Entrez Gene: 58208 Mouse
- Omim: 606558 Human
- SwissProt: Q9C0K0 Human
- SwissProt: Q99PV8 Mouse
- Unigene: 709690 Human
- Unigene: 392694 Mouse
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Alternative names
- ATL1 alpha antibody
- ATL1 antibody
- ATL1 beta antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ctip2 antibody (ab70452)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human stomach carcinoma tissue labelling Ctip2 with ab70452 at 1/1000 (1µg/ml). Detection: DAB.
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Western blot - Anti-Ctip2 antibody (ab70452)All lanes : Anti-Ctip2 antibody (ab70452) at 0.1 µg/ml
Lane 1 : Jurkat cell nuclear extract at 5 µg
Lane 2 : Jurkat cell nuclear extract at 50 µg
Predicted band size: 99 kDa
Observed band size: 120-130 kDa why is the actual band size different from the predicted? -
Immunoprecipitation - Anti-Ctip2 antibody (ab70452)All lanes: 1mg of nuclear extract from Jurkat cell treated with ab70452 at 0.5µg/ml.
Lane 1: ab70452 used at 1 µg/mg lysate for IP.
Lane 2: ab70452 used at 10 µg/mg lysate for IP.
Lane 3: an alternate Ctip2 antibody (ab70453) used at 1 µg/mg lysate for IP.
Lane 4: an alternate Ctip2 antibody (ab70453) used at 10 µg/mg lysate for IP.
Normal rabbit IgG was used as a control for immunoprecipitation reactions. Detection:
Chemiluminescence with exposure time of 30 seconds.
Protocols
References (1)
ab70452 has been referenced in 1 publication.
- Maskari RA et al. Functional characterization of common BCL11B gene desert variants suggests a lymphocyte-mediated association of BCL11B with aortic stiffness. Eur J Hum Genet 26:1648-1657 (2018). PubMed: 30089823
