- Datasheet
- Specific References
- Protocols
Overview
-
Product nameAnti-Ctip2 antibody
See all Ctip2 primary antibodies -
DescriptionRabbit polyclonal to Ctip2
-
Host speciesRabbit
-
Tested applicationsSuitable for: IHC-P, WB, IPmore details
-
Species reactivityReacts with: Human
Predicted to work with: Mouse, Cow, Pig, Chimpanzee, Baboon, Rhesus monkey, Gorilla, Orangutan, Bat
-
Immunogen
Synthetic peptide corresponding to a region between residues 500 and 550 of human Ctip2 (Swiss-Prot Q9C0K0).
-
Positive control
- Nuclear extract from Jurkat cells
Properties
-
FormLiquid
-
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
-
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS -
Concentration information loading... -
PurityAffinity purified
-
Purification notesab70452 was affinity purified using an epitope specific to Ctip2 immobilized on solid support.
-
ClonalityPolyclonal
-
IsotypeIgG
-
Research areas
Associated products
-
Compatible Secondaries
Applications
Our Abpromise guarantee covers the use of ab70452 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | 1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. | |
| WB | 1/5000 - 1/15000. Detects a band of approximately 120 kDa (predicted molecular weight: 99 kDa). | |
| IP | Use at 1-5 µg/mg of lysate. |
Target
-
FunctionTumor-suppressor protein involved in T-cell lymphomas. May function on the P53-signaling pathway. May be a key regulator of both differentiation and survival during thymocyte development. Repress transcription through direct, TFCOUP2-independent binding to a GC-rich response element.
-
Tissue specificityHighly expressed in brain and in malignant T-cell lines derived from patients with adult T-cell leukemia/lymphoma.
-
Sequence similaritiesContains 6 C2H2-type zinc fingers.
-
Cellular localizationNucleus.
- Information by UniProt
-
Database links
- Entrez Gene: 64919 Human
- Entrez Gene: 58208 Mouse
- Omim: 606558 Human
- SwissProt: Q9C0K0 Human
- SwissProt: Q99PV8 Mouse
- Unigene: 709690 Human
- Unigene: 392694 Mouse
-
Alternative names
- ATL1 alpha antibody
- ATL1 antibody
- ATL1 beta antibody
see all
Images
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ctip2 antibody (ab70452)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human stomach carcinoma tissue labelling Ctip2 with ab70452 at 1/1000 (1µg/ml). Detection: DAB. -
Western blot - Anti-Ctip2 antibody (ab70452)All lanes : Anti-Ctip2 antibody (ab70452) at 0.1 µg/ml
Lane 1 : Jurkat cell nuclear extract at 5 µg
Lane 2 : Jurkat cell nuclear extract at 50 µg
Predicted band size: 99 kDa
Observed band size: 120-130 kDa (why is the actual band size different from the predicted?) -
Immunoprecipitation - Anti-Ctip2 antibody (ab70452)All lanes: 1mg of nuclear extract from Jurkat cell treated with ab70452 at 0.5µg/ml.
Lane 1: ab70452 used at 1 µg/mg lysate for IP.
Lane 2: ab70452 used at 10 µg/mg lysate for IP.
Lane 3: an alternate Ctip2 antibody (ab70453) used at 1 µg/mg lysate for IP.
Lane 4: an alternate Ctip2 antibody (ab70453) used at 10 µg/mg lysate for IP.
Normal rabbit IgG was used as a control for immunoprecipitation reactions. Detection:
Chemiluminescence with exposure time of 30 seconds.
References
ab70452 has not yet been referenced specifically in any publications.
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"