Product nameAnti-Ctip2 antibody
See all Ctip2 primary antibodies
DescriptionRabbit polyclonal to Ctip2
Tested applicationsSuitable for: WB, IP, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rabbit, Guinea pig, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Xenopus tropicalis
Synthetic peptide corresponding to a region between C terminal residues 850-894 of human Ctip2 (Swiss-Prot Q9C0K0).
- Nuclear extract from Jurkat cells
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab70453 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/15000. Detects a band of approximately 120-130 kDa (predicted molecular weight: 100 kDa).|
|IP||Use at 1-5 µg/mg of lysate.|
|IHC-P||1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionTumor-suppressor protein involved in T-cell lymphomas. May function on the P53-signaling pathway. May be a key regulator of both differentiation and survival during thymocyte development. Repress transcription through direct, TFCOUP2-independent binding to a GC-rich response element.
Tissue specificityHighly expressed in brain and in malignant T-cell lines derived from patients with adult T-cell leukemia/lymphoma.
Sequence similaritiesContains 6 C2H2-type zinc fingers.
- Information by UniProt
- ATL1 alpha antibody
- ATL1 antibody
- ATL1 beta antibody
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate carcinoma tissue labelling Ctip2 with ab70453 at 1/1000 (1µg/ml). Detection: DAB.
All lanes: 1mg nuclear extract from Jurkat cells analysed on a blot using ab70453 at 0.5µg/ml.
Lane 1: an alternate Ctip2 antibody used at 1 µg/mg lysate for IP.
Lane 2: The same alternate antibody used at 10 µg/mg lysate for IP.
Lane 3: ab70453 used at 1 µg/mg lysate for IP.
Lane 4: ab70453 at 10 µg/mg lysate for IP.
Normal rabbit IgG was used as a control for immunoprecipitation reactions.
All lanes : Anti-Ctip2 antibody (ab70453) at 0.1 µg/ml
Lane 1 : Jurkat cell nuclear extract at 5 µg
Lane 2 : Jurkat cell nuclear extract at 50 µg
Predicted band size: 100 kDa
Observed band size: 120-130 kDa why is the actual band size different from the predicted?
All lanes : Anti-Ctip2 antibody (ab70453) at 0.2 µg/ml
Lane 1 : L1210 whole cell lysate
Lane 2 : TCMK-1 whole cell lysate
Lane 3 : 4T1 whole cell lysate
Lane 4 : CT26.WT whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 100 kDa
Exposure time: 3 minutes
This product has been referenced in:
- Forbes LH & Andrews MR Grafted Human iPSC-Derived Neural Progenitor Cells Express Integrins and Extend Long-Distance Axons Within the Developing Corticospinal Tract. Front Cell Neurosci 13:26 (2019). Read more (PubMed: 30809126) »
- Baldauf MC et al. Robust diagnosis of Ewing sarcoma by immunohistochemical detection of super-enhancer-driven EWSR1-ETS targets. Oncotarget 9:1587-1601 (2018). Read more (PubMed: 29416716) »