Recombinant Anti-Ctip2 antibody [EPR12275(2)] - BSA and Azide free (ab243191)
![Western blot - Anti-Ctip2 antibody [EPR12275(2)] - BSA and Azide free (ab243191)](https://www.abcam.com/ps/products/243/ab243191/Images/ab243191-398976-anti-ctip2-antibody-epr12275-2-bsa-and-azide-free-western-blot.jpg "Western blot - Anti-Ctip2 antibody [EPR12275(2)] - BSA and Azide free (ab243191)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR12275(2)] to Ctip2 - BSA and Azide free
- Suitable for: WB
- Reacts with: Human
Overview
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Product name
Anti-Ctip2 antibody [EPR12275(2)] - BSA and Azide free
See all Ctip2 primary antibodies -
Description
Rabbit monoclonal [EPR12275(2)] to Ctip2 - BSA and Azide free -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Molt-4, Jurkat, HuT-78 and Human fetal brain lysates.
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General notes
Ab243191 is the carrier-free version of ab187668. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
ab243191 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR12275(2) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Conjugation kits
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab243191 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| Application | Species |
|---|---|
| WB |
Human
|
| Application | Abreviews | Notes |
|---|---|---|
| WB |
Use at an assay dependent concentration. Detects a band of approximately 130 kDa (predicted molecular weight: 96 kDa).
|
| Notes |
|---|
|
WB
Use at an assay dependent concentration. Detects a band of approximately 130 kDa (predicted molecular weight: 96 kDa). |
Target
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Function
Tumor-suppressor protein involved in T-cell lymphomas. May function on the P53-signaling pathway. May be a key regulator of both differentiation and survival during thymocyte development. Repress transcription through direct, TFCOUP2-independent binding to a GC-rich response element. -
Tissue specificity
Highly expressed in brain and in malignant T-cell lines derived from patients with adult T-cell leukemia/lymphoma. -
Sequence similarities
Contains 6 C2H2-type zinc fingers. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 64919 Human
- Omim: 606558 Human
- SwissProt: Q9C0K0 Human
- Unigene: 709690 Human
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Alternative names
- ATL1 alpha antibody
- ATL1 antibody
- ATL1 beta antibody
see all
Images
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Western blot - Anti-Ctip2 antibody [EPR12275(2)] - BSA and Azide free (ab243191)All lanes : Anti-Ctip2 antibody [EPR12275(2)] (ab187668) at 1/10000 dilution
Lane 1 : MOLT4 cell lysate
Lane 2 : Jurkat cell lysate
Lane 3 : HuT-78 cell lysate
Lane 4 : Human fetal brain cell lysate
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 96 kDa
Observed band size: 130 kDa why is the actual band size different from the predicted?This data was developed using ab187668, the same antibody clone in a different buffer formulation.
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![Anti-Ctip2 antibody [EPR12275(2)] - BSA and Azide free (ab243191)](https://www.abcam.com/ps/products/243/ab243191/Images/ab243191-2-benefits-of-recombinant-antibodies.png)
Anti-Ctip2 antibody [EPR12275(2)] - BSA and Azide free (ab243191)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
Certificate of Compliance
References (0)
ab243191 has not yet been referenced specifically in any publications.
