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Epigenetics and Nuclear Signaling Chromatin Modifying Enzymes Acetylation
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RecombinantRabMAb

Recombinant Anti-Ctip2 antibody [EPR12275(2)] - BSA and Azide free (ab243191)

  • Datasheet
  • Certificate of Compliance
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Western blot - Anti-Ctip2 antibody [EPR12275(2)] - BSA and Azide free (ab243191)
  • Anti-Ctip2 antibody [EPR12275(2)] - BSA and Azide free (ab243191)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR12275(2)] to Ctip2 - BSA and Azide free
  • Suitable for: WB
  • Reacts with: Human

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Conjugation
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PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918)

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Overview

  • Product name

    Anti-Ctip2 antibody [EPR12275(2)] - BSA and Azide free
    See all Ctip2 primary antibodies
  • Description

    Rabbit monoclonal [EPR12275(2)] to Ctip2 - BSA and Azide free
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Molt-4, Jurkat, HuT-78 and Human fetal brain lysates.
  • General notes

    Ab243191 is the carrier-free version of ab187668. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab243191 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • Storage buffer

    pH: 7.2
    Constituent: PBS
  • Carrier free

    Yes
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR12275(2)
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • Chromatin Modifying Enzymes
    • Acetylation
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Domain Families
    • Zinc Finger
    • Neuroscience
    • Neurology process
    • Neural Signal Transduction
    • Neuroscience
    • Neurology process
    • Neurogenesis
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Transcription Factors
    • Epigenetics and Nuclear Signaling
    • Chromatin Modifying Enzymes
    • Acetylation
    • HAT

Associated products

  • Alternative Versions

    • Anti-Ctip2 antibody [EPR12275(2)] (ab187668)
  • Conjugation kits

    • PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918)
    • APC Conjugation Kit - Lightning-Link® (ab201807)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Positive Controls

    • Jurkat whole cell lysate (ab7899)
    • MOLT-4 whole cell lysate (ab7912)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab243191 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
WB
Human
Application Abreviews Notes
WB
Use at an assay dependent concentration. Detects a band of approximately 130 kDa (predicted molecular weight: 96 kDa).
Notes
WB
Use at an assay dependent concentration. Detects a band of approximately 130 kDa (predicted molecular weight: 96 kDa).

Target

  • Function

    Tumor-suppressor protein involved in T-cell lymphomas. May function on the P53-signaling pathway. May be a key regulator of both differentiation and survival during thymocyte development. Repress transcription through direct, TFCOUP2-independent binding to a GC-rich response element.
  • Tissue specificity

    Highly expressed in brain and in malignant T-cell lines derived from patients with adult T-cell leukemia/lymphoma.
  • Sequence similarities

    Contains 6 C2H2-type zinc fingers.
  • Cellular localization

    Nucleus.
  • Target information above from: UniProt accession Q9C0K0 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 64919 Human
    • Omim: 606558 Human
    • SwissProt: Q9C0K0 Human
    • Unigene: 709690 Human
    • Alternative names

      • ATL1 alpha antibody
      • ATL1 antibody
      • ATL1 beta antibody
      • ATL1 gamma antibody
      • ATL1-delta antibody
      • B cell CLL/lymphoma 11B/T cell receptor delta constant region fusion protein antibody
      • B cell lymphoma/leukemia 11B antibody
      • B-cell CLL/lymphoma 11B (zinc finger protein) antibody
      • B-cell CLL/lymphoma 11B antibody
      • B-cell lymphoma/leukemia 11B antibody
      • BC11B_HUMAN antibody
      • BCL-11B antibody
      • Bcl11b antibody
      • BCL11B/TRDC fusion antibody
      • COUP TF interacting protein 2 antibody
      • COUP-TF interacting protein 2 antibody
      • COUP-TF-interacting protein 2 antibody
      • Ctip 2 antibody
      • CTIP-2 antibody
      • CTIP2 antibody
      • hRIT1 alpha antibody
      • hRit1 antibody
      • Radiation induced tumor suppressor gene 1 antibody
      • Radiation induced tumor suppressor gene 1 protein antibody
      • Radiation-induced tumor suppressor gene 1 protein antibody
      • Rit 1 antibody
      • Rit1 antibody
      • zinc finger protein hRit1 alpha antibody
      • ZNF856B antibody
      see all

    Images

    • Western blot - Anti-Ctip2 antibody [EPR12275(2)] - BSA and Azide free (ab243191)
      Western blot - Anti-Ctip2 antibody [EPR12275(2)] - BSA and Azide free (ab243191)
      All lanes : Anti-Ctip2 antibody [EPR12275(2)] (ab187668) at 1/10000 dilution

      Lane 1 : MOLT4 cell lysate
      Lane 2 : Jurkat cell lysate
      Lane 3 : HuT-78 cell lysate
      Lane 4 : Human fetal brain cell lysate

      Secondary
      All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

      Predicted band size: 96 kDa
      Observed band size: 130 kDa
      why is the actual band size different from the predicted?



      This data was developed using ab187668, the same antibody clone in a different buffer formulation.

       

    • Anti-Ctip2 antibody [EPR12275(2)] - BSA and Azide free (ab243191)
      Anti-Ctip2 antibody [EPR12275(2)] - BSA and Azide free (ab243191)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet

    Certificate of Compliance

    To download a Certificate of Compliance, please enter your Lot number below:

  • References (0)

    Publishing research using ab243191? Please let us know so that we can cite the reference in this datasheet.

    ab243191 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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