Product nameAnti-Ctk1 antibody
DescriptionRabbit polyclonal to Ctk1
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Saccharomyces cerevisiae
- This antibody gave a positive signal in S.cerevisiae (Y190) Whole Cell Lysate
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab47812 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 58 kDa (predicted molecular weight: 60 kDa).|
RelevanceCtk1 is the catalytic subunit of CTDK-1 that hyperphosphorylates the C-terminal repeat domain (CTD) of RNA polymerase II largest subunit. CTDK-I is localized to the nucleolus. Ctk1 also co-precipitates with RNA polymerase I co-indicating a direct interaction between RNA polymerase I and Ctk1. Therefore, CTDK-I appears to play a role regulating distinct nuclear transcriptional machineries.
- CTD kinase 58 kDa subunit antibody
- CTD kinase subunit 1 antibody
- CTD kinase subunit alpha antibody
- CTDK I alpha subunit antibody
Anti-Ctk1 antibody (ab47812) at 1 µg/ml + S.cerevisiae (Y190) Whole Cell Lysate at 10 µg
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 60 kDa
Observed band size: 58 kDa why is the actual band size different from the predicted?
Additional bands at: 110 kDa, 120 kDa, 25 kDa, 65 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 8 minutes
ab47812 has not yet been referenced specifically in any publications.