Key features and details
- Rabbit polyclonal to Ctk1
- Suitable for: WB
- Reacts with: Saccharomyces cerevisiae
- Isotype: IgG
Product nameAnti-Ctk1 antibody
DescriptionRabbit polyclonal to Ctk1
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Saccharomyces cerevisiae
- This antibody gave a positive signal in S.cerevisiae (Y190) Whole Cell Lysate
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab47812 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 58 kDa (predicted molecular weight: 60 kDa).|
RelevanceCtk1 is the catalytic subunit of CTDK-1 that hyperphosphorylates the C-terminal repeat domain (CTD) of RNA polymerase II largest subunit. CTDK-I is localized to the nucleolus. Ctk1 also co-precipitates with RNA polymerase I co-indicating a direct interaction between RNA polymerase I and Ctk1. Therefore, CTDK-I appears to play a role regulating distinct nuclear transcriptional machineries.
- CTD kinase 58 kDa subunit antibody
- CTD kinase subunit 1 antibody
- CTD kinase subunit alpha antibody
- CTDK I alpha subunit antibody
Anti-Ctk1 antibody (ab47812) at 1 µg/ml + S.cerevisiae (Y190) Whole Cell Lysate at 10 µg
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 60 kDa
Observed band size: 58 kDa why is the actual band size different from the predicted?
Additional bands at: 110 kDa, 120 kDa, 25 kDa, 65 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 8 minutes
ab47812 has not yet been referenced specifically in any publications.