Recombinant
RabMAb

Anti-CTLA4 antibody [EPR1476] (ab134090)

Overview

  • Product name
    Anti-CTLA4 antibody [EPR1476]
    See all CTLA4 primary antibodies
  • Description
    Rabbit monoclonal [EPR1476] to CTLA4
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, ICC/IFmore details
    Unsuitable for: IHC-P
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to Human CTLA4 aa 1-100 (N terminal).
    (Peptide available as ab215388)

  • Positive control
    • HeLa, Raji, Molt-4, and K562 cell lysates
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab134090 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/5000. Predicted molecular weight: 25 kDa.Can be blocked with Human CTLA4 peptide (ab215388).
ICC/IF 1/200.
  • Application notes
    Is unsuitable for IHC-P.
  • Target

    • Function
      Inhibitory receptor acting as a major negative regulator of T-cell responses. The affinity of CTLA4 for its natural B7 family ligands, CD80 and CD86, is considerably stronger than the affinity of their cognate stimulatory coreceptor CD28.
    • Tissue specificity
      Widely expressed with highest levels in lymphoid tissues. Detected in activated T-cells where expression levels are 30- to 50-fold less than CD28, the stimulatory coreceptor, on the cell surface following activation.
    • Involvement in disease
      Genetic variation in CTLA4 influences susceptibility to systemic lupus erythematosus (SLE) [MIM:152700]. SLE is a chronic, inflammatory and often febrile multisystemic disorder of connective tissue. It affects principally the skin, joints, kidneys and serosal membranes. SLE is thought to represent a failure of the regulatory mechanisms of the autoimmune system.
      Note=Genetic variations in CTLA4 may influence susceptibility to Graves disease, an autoimmune disorder associated with overactivity of the thyroid gland and hyperthyroidism.
      Genetic variation in CTLA4 is the cause of susceptibility to diabetes mellitus insulin-dependent type 12 (IDDM12) [MIM:601388]. A multifactorial disorder of glucose homeostasis that is characterized by susceptibility to ketoacidosis in the absence of insulin therapy. Clinical fetaures are polydipsia, polyphagia and polyuria which result from hyperglycemia-induced osmotic diuresis and secondary thirst. These derangements result in long-term complications that affect the eyes, kidneys, nerves, and blood vessels.
      Genetic variation in CTLA4 is the cause of susceptibility to celiac disease type 3 (CELIAC3) [MIM:609755]. It is a multifactorial disorder of the small intestine that is influenced by both environmental and genetic factors. It is characterized by malabsorption resulting from inflammatory injury to the mucosa of the small intestine after the ingestion of wheat gluten or related rye and barley proteins. In its classic form, celiac disease is characterized in children by malabsorption and failure to thrive.
    • Sequence similarities
      Contains 1 Ig-like V-type (immunoglobulin-like) domain.
    • Post-translational
      modifications
      N-glycosylation is important for dimerization.
      Phosphorylation at Tyr-201 prevents binding to the AP-2 adapter complex, blocks endocytosis, and leads to retention of CTLA4 on the cell surface.
    • Cellular localization
      Cell membrane. Exists primarily an intracellular antigen whose surface expression is tightly regulated by restricted trafficking to the cell surface and rapid internalisation and.
    • Information by UniProt
    • Database links
    • Alternative names
      • ALPS5 antibody
      • CD 152 antibody
      • CD antibody
      • CD152 antibody
      • CD152 antigen antibody
      • CD152 isoform antibody
      • Celiac disease 3 antibody
      • CELIAC3 antibody
      • CTLA 4 antibody
      • CTLA-4 antibody
      • CTLA4 antibody
      • CTLA4_HUMAN antibody
      • Cytotoxic T cell associated 4 antibody
      • Cytotoxic T lymphocyte antigen 4 antibody
      • Cytotoxic T lymphocyte associated 4 antibody
      • Cytotoxic T lymphocyte associated 4, soluble isoform, included antibody
      • Cytotoxic T lymphocyte associated antigen 4 antibody
      • Cytotoxic T lymphocyte associated antigen 4 short spliced form antibody
      • Cytotoxic T lymphocyte associated protein 4 antibody
      • Cytotoxic T lymphocyte associated serine esterase 4 antibody
      • Cytotoxic T lymphocyte protein 4 antibody
      • Cytotoxic T-lymphocyte protein 4 antibody
      • Cytotoxic T-lymphocyte-associated antigen 4 antibody
      • GRD4 antibody
      • GSE antibody
      • ICOS antibody
      • IDDM12 antibody
      • insulin-dependent diabetes mellitus 12 antibody
      • Ligand and transmembrane spliced cytotoxic T lymphocyte associated antigen 4 antibody
      • OTTHUMP00000216623 antibody
      see all

    Images

    • Immunofluorescence staining of Raji cells with purified ab134090 at a working dilution of 1/250, counter-stained with DAPI. The secondary antibody was Alexa Fluor® 488 goat anti rabbit (ab150077), used at a dilution of 1/1000. The cells were fixed in 100% Methanol and permeabilized using 0.1% Triton X 100. The negative control is shown in bottom right hand panel.

    • All lanes : Anti-CTLA4 antibody [EPR1476] (ab134090) at 1/5000 dilution (purified)

      Lane 1 : Raji cell lysate
      Lane 2 : K562 cell lysate
      Lane 3 : TF-1 cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : HRP goat anti-rabbit (H+L) at 1/1000 dilution

      Predicted band size: 25 kDa
      Additional bands at: 43 kDa (possible glycosylated form)



      Blocking buffer: 5% NFDM/TBST

      Dilution buffer: 5% NFDM/TBST

    • Anti-CTLA4 antibody [EPR1476] (ab134090) at 1/10000 dilution (purified) + C6 cell lysate at 10 µg

      Secondary
      HRP goat anti-rabbit (H+L) at 1/1000 dilution

      Predicted band size: 25 kDa
      Additional bands at: 43 kDa (possible glycosylated form)



      Blocking buffer: 5% NFDM/TBST

      Dilution buffer: 5% NFDM/TBST

    • Anti-CTLA4 antibody [EPR1476] (ab134090) at 1/10000 dilution (purified) + NIH/3T3 cell lysate at 10 µg

      Secondary
      HRP goat anti-rabbit (H+L) at 1/1000 dilution

      Predicted band size: 25 kDa
      Additional bands at: 43 kDa (possible glycosylated form)



      Blocking buffer: 5% NFDM/TBST

      Dilution buffer: 5% NFDM/TBST

    • All lanes : Anti-CTLA4 antibody [EPR1476] (ab134090) (unpurified)

      Lane 1 : Jurkat cell lysate
      Lane 2 : Raji cell lysate
      Lane 3 : Human CTLA4 full length protein (ab69787)

      Predicted band size: 25 kDa

    References

    This product has been referenced in:
    • Zhou J  et al. Prognostic and therapeutic value of CD103+ cells in renal cell carcinoma. Exp Ther Med 15:4979-4986 (2018). Read more (PubMed: 29805521) »
    • Giles AJ  et al. Dexamethasone-induced immunosuppression: mechanisms and implications for immunotherapy. J Immunother Cancer 6:51 (2018). WB ; Human . Read more (PubMed: 29891009) »

    See all 3 Publications for this product

    Customer reviews and Q&As

    Merci de nous avoir contactés.

    Anti-AP2M1(ab75995) a été testé sur des lysats de cellules MCF-7 et SKBR-3. Anti- CTLA4 (ab134090) a été testé sur des lysats de cellules HELA, Raji, MOLT4 et K56...

    Read More

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