Recombinant
RabMAb

Anti-CTLA4 antibody [EPR1476] (Alexa Fluor® 488) (ab216382)

Overview

  • Product name
    Anti-CTLA4 antibody [EPR1476] (Alexa Fluor® 488)
    See all CTLA4 primary antibodies
  • Description
    Rabbit monoclonal [EPR1476] to CTLA4 (Alexa Fluor® 488)
  • Host species
    Rabbit
  • Conjugation
    Alexa Fluor® 488. Ex: 495nm, Em: 519nm
  • Tested applications
    Suitable for: ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide within Human CTLA4 aa 1-100 (N terminal). The exact sequence is proprietary.
    Database link: P16410

  • Positive control
    • ICC/IF: Raji cells
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab216382 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/1000.

This product gave a positive signal in Raji cells fixed with 80% methanol (5 minutes)

Target

  • Function
    Inhibitory receptor acting as a major negative regulator of T-cell responses. The affinity of CTLA4 for its natural B7 family ligands, CD80 and CD86, is considerably stronger than the affinity of their cognate stimulatory coreceptor CD28.
  • Tissue specificity
    Widely expressed with highest levels in lymphoid tissues. Detected in activated T-cells where expression levels are 30- to 50-fold less than CD28, the stimulatory coreceptor, on the cell surface following activation.
  • Involvement in disease
    Genetic variation in CTLA4 influences susceptibility to systemic lupus erythematosus (SLE) [MIM:152700]. SLE is a chronic, inflammatory and often febrile multisystemic disorder of connective tissue. It affects principally the skin, joints, kidneys and serosal membranes. SLE is thought to represent a failure of the regulatory mechanisms of the autoimmune system.
    Note=Genetic variations in CTLA4 may influence susceptibility to Graves disease, an autoimmune disorder associated with overactivity of the thyroid gland and hyperthyroidism.
    Genetic variation in CTLA4 is the cause of susceptibility to diabetes mellitus insulin-dependent type 12 (IDDM12) [MIM:601388]. A multifactorial disorder of glucose homeostasis that is characterized by susceptibility to ketoacidosis in the absence of insulin therapy. Clinical fetaures are polydipsia, polyphagia and polyuria which result from hyperglycemia-induced osmotic diuresis and secondary thirst. These derangements result in long-term complications that affect the eyes, kidneys, nerves, and blood vessels.
    Genetic variation in CTLA4 is the cause of susceptibility to celiac disease type 3 (CELIAC3) [MIM:609755]. It is a multifactorial disorder of the small intestine that is influenced by both environmental and genetic factors. It is characterized by malabsorption resulting from inflammatory injury to the mucosa of the small intestine after the ingestion of wheat gluten or related rye and barley proteins. In its classic form, celiac disease is characterized in children by malabsorption and failure to thrive.
  • Sequence similarities
    Contains 1 Ig-like V-type (immunoglobulin-like) domain.
  • Post-translational
    modifications
    N-glycosylation is important for dimerization.
    Phosphorylation at Tyr-201 prevents binding to the AP-2 adapter complex, blocks endocytosis, and leads to retention of CTLA4 on the cell surface.
  • Cellular localization
    Cell membrane. Exists primarily an intracellular antigen whose surface expression is tightly regulated by restricted trafficking to the cell surface and rapid internalisation and.
  • Information by UniProt
  • Database links
  • Alternative names
    • ALPS5 antibody
    • CD 152 antibody
    • CD antibody
    • CD152 antibody
    • CD152 antigen antibody
    • CD152 isoform antibody
    • Celiac disease 3 antibody
    • CELIAC3 antibody
    • CTLA 4 antibody
    • CTLA-4 antibody
    • CTLA4 antibody
    • CTLA4_HUMAN antibody
    • Cytotoxic T cell associated 4 antibody
    • Cytotoxic T lymphocyte antigen 4 antibody
    • Cytotoxic T lymphocyte associated 4 antibody
    • Cytotoxic T lymphocyte associated 4, soluble isoform, included antibody
    • Cytotoxic T lymphocyte associated antigen 4 antibody
    • Cytotoxic T lymphocyte associated antigen 4 short spliced form antibody
    • Cytotoxic T lymphocyte associated protein 4 antibody
    • Cytotoxic T lymphocyte associated serine esterase 4 antibody
    • Cytotoxic T lymphocyte protein 4 antibody
    • Cytotoxic T-lymphocyte protein 4 antibody
    • Cytotoxic T-lymphocyte-associated antigen 4 antibody
    • GRD4 antibody
    • GSE antibody
    • ICOS antibody
    • IDDM12 antibody
    • insulin-dependent diabetes mellitus 12 antibody
    • Ligand and transmembrane spliced cytotoxic T lymphocyte associated antigen 4 antibody
    • OTTHUMP00000216623 antibody
    see all

Images

  • ab216382 staining CTLA4 in Raji cells. The cells were fixed with 80% methanol (5 minutes), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1 hour. The cells were then incubated overnight at +4°C with ab216382 at 1/1000 dilution (shown in green) and ab195884, Rat monoclonal to Tubulin (Alexa Fluor® 647), at 1/250 dilution (shown in red). Nuclear DNA was labeled with DAPI (shown in blue).

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

References

ab216382 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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