Recombinant
RabMAb

Recombinant Anti-CTLA4 antibody [EPR21972-205] - BSA and Azide free (ab237017)

Overview

  • Product name

    Anti-CTLA4 antibody [EPR21972-205] - BSA and Azide free
    See all CTLA4 primary antibodies
  • Description

    Rabbit monoclonal [EPR21972-205] to CTLA4 - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: Flow Cytmore details
  • Species reactivity

    Reacts with: Mouse
  • Immunogen

    Recombinant fragment within Mouse CTLA4 aa 1-200. The exact sequence is proprietary.
    Database link: P09793

  • Positive control

    • Flow Cyt: Mouse primary splenocytes treated with ConA .
  • General notes

    Ab237017 is the carrier-free version of ab224295. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab237017 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab237017 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use at an assay dependent concentration.

Target

  • Function

    Inhibitory receptor acting as a major negative regulator of T-cell responses. The affinity of CTLA4 for its natural B7 family ligands, CD80 and CD86, is considerably stronger than the affinity of their cognate stimulatory coreceptor CD28.
  • Tissue specificity

    Widely expressed with highest levels in lymphoid tissues. Detected in activated T-cells where expression levels are 30- to 50-fold less than CD28, the stimulatory coreceptor, on the cell surface following activation.
  • Involvement in disease

    Genetic variation in CTLA4 influences susceptibility to systemic lupus erythematosus (SLE) [MIM:152700]. SLE is a chronic, inflammatory and often febrile multisystemic disorder of connective tissue. It affects principally the skin, joints, kidneys and serosal membranes. SLE is thought to represent a failure of the regulatory mechanisms of the autoimmune system.
    Note=Genetic variations in CTLA4 may influence susceptibility to Graves disease, an autoimmune disorder associated with overactivity of the thyroid gland and hyperthyroidism.
    Genetic variation in CTLA4 is the cause of susceptibility to diabetes mellitus insulin-dependent type 12 (IDDM12) [MIM:601388]. A multifactorial disorder of glucose homeostasis that is characterized by susceptibility to ketoacidosis in the absence of insulin therapy. Clinical fetaures are polydipsia, polyphagia and polyuria which result from hyperglycemia-induced osmotic diuresis and secondary thirst. These derangements result in long-term complications that affect the eyes, kidneys, nerves, and blood vessels.
    Genetic variation in CTLA4 is the cause of susceptibility to celiac disease type 3 (CELIAC3) [MIM:609755]. It is a multifactorial disorder of the small intestine that is influenced by both environmental and genetic factors. It is characterized by malabsorption resulting from inflammatory injury to the mucosa of the small intestine after the ingestion of wheat gluten or related rye and barley proteins. In its classic form, celiac disease is characterized in children by malabsorption and failure to thrive.
  • Sequence similarities

    Contains 1 Ig-like V-type (immunoglobulin-like) domain.
  • Post-translational
    modifications

    N-glycosylation is important for dimerization.
    Phosphorylation at Tyr-201 prevents binding to the AP-2 adapter complex, blocks endocytosis, and leads to retention of CTLA4 on the cell surface.
  • Cellular localization

    Cell membrane. Exists primarily an intracellular antigen whose surface expression is tightly regulated by restricted trafficking to the cell surface and rapid internalisation and.
  • Information by UniProt
  • Database links

  • Alternative names

    • ALPS5 antibody
    • CD 152 antibody
    • CD antibody
    • CD152 antibody
    • CD152 antigen antibody
    • CD152 isoform antibody
    • Celiac disease 3 antibody
    • CELIAC3 antibody
    • CTLA 4 antibody
    • CTLA-4 antibody
    • CTLA4 antibody
    • CTLA4_HUMAN antibody
    • Cytotoxic T cell associated 4 antibody
    • Cytotoxic T lymphocyte antigen 4 antibody
    • Cytotoxic T lymphocyte associated 4 antibody
    • Cytotoxic T lymphocyte associated 4, soluble isoform, included antibody
    • Cytotoxic T lymphocyte associated antigen 4 antibody
    • Cytotoxic T lymphocyte associated antigen 4 short spliced form antibody
    • Cytotoxic T lymphocyte associated protein 4 antibody
    • Cytotoxic T lymphocyte associated serine esterase 4 antibody
    • Cytotoxic T lymphocyte protein 4 antibody
    • Cytotoxic T-lymphocyte protein 4 antibody
    • Cytotoxic T-lymphocyte-associated antigen 4 antibody
    • GRD4 antibody
    • GSE antibody
    • ICOS antibody
    • IDDM12 antibody
    • insulin-dependent diabetes mellitus 12 antibody
    • Ligand and transmembrane spliced cytotoxic T lymphocyte associated antigen 4 antibody
    • OTTHUMP00000216623 antibody
    see all

Images

  • Flow cytometric analysis of mouse primary splenocytes treated with ConA (2.5 μg/ml, 3 days) labeling CTLA4 with ab224295 at 1/100 dilution (Right panel) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (Left panel). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

    A low level of CTLA4 is expected to be on the cell surface; the majority is intracellular, localized to secretory lysosomes (PMID: 11046036). Gated on viable cells.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab224295).

     

References

ab237017 has not yet been referenced specifically in any publications.

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