Anti-CTNNA1 antibody [1G5] (ab231306)
- Datasheet
- References (2)
- Protocols
Overview
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Product name
Anti-CTNNA1 antibody [1G5]
See all CTNNA1 primary antibodies -
Description
Mouse monoclonal [1G5] to CTNNA1 -
Host species
Mouse -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Rat, Human -
Immunogen
Recombinant full length protein corresponding to Human CTNNA1. (A recombinant maltose binding protein fused to full length human alpha catenin).
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Positive control
- ICC/IF: A431 cells WB: HAP1, rat colon
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General notes
This antibody clone is manufactured by Abcam. If you require it in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com.
This product was previously labelled as alpha 1 Catenin
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
1G5 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
Our Abpromise guarantee covers the use of ab231306 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use a concentration of 1 µg/ml. Predicted molecular weight: 100 kDa. | |
| ICC/IF | Use a concentration of 5 µg/ml. This antibody gives positive signal in 4% PFA and 100% MeOH-fixed cells. |
Target
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Function
Associates with the cytoplasmic domain of a variety of cadherins. The association of catenins to cadherins produces a complex which is linked to the actin filament network, and which seems to be of primary importance for cadherins cell-adhesion properties. Can associate with both E- and N-cadherins. Originally believed to be a stable component of E-cadherin/catenin adhesion complexes and to mediate the linkage of cadherins to the actin cytoskeleton at adherens junctions. In contrast, cortical actin was found to be much more dynamic than E-cadherin/catenin complexes and CTNNA1 was shown not to bind to F-actin when assembled in the complex suggesting a different linkage between actin and adherens junctions components. The homodimeric form may regulate actin filament assembly and inhibit actin branching by competing with the Arp2/3 complex for binding to actin filaments. May play a crucial role in cell differentiation. -
Tissue specificity
Expressed ubiquitously in normal tissues. -
Sequence similarities
Belongs to the vinculin/alpha-catenin family. -
Post-translational
modificationsSumoylated. -
Cellular localization
Cell membrane and Cytoplasm > cytoskeleton. Cell junction > adherens junction. Cell membrane. Cell junction. Found at cell-cell boundaries and probably at cell-matrix boundaries. - Information by UniProt
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Database links
- Entrez Gene: 1495 Human
- Entrez Gene: 307505 Rat
- Omim: 116805 Human
- SwissProt: P35221 Human
- Unigene: 445981 Human
- Unigene: 656653 Human
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Alternative names
- Alpha E-catenin antibody
- Cadherin associated protein 102kDa antibody
- Cadherin associated protein antibody
see all
Images
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![Immunocytochemistry/ Immunofluorescence - Anti-CTNNA1 antibody [1G5] (ab231306)](https://www.abcam.com/ps/products/231/ab231306/Images/ab231306-324645-anti-alpha-1-catenin-antibody-1g5-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-CTNNA1 antibody [1G5] (ab231306)ab231306 staining CTNNA1 in A431 cells. The cells were fixed with 100% methanol (5min), permeabilized with 0.1%PBS-Tween for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab231306 at 5μg/ml and ab6046, Rabbit polyclonal to beta Tubulin - Loading Control, at 1/1000 dilution. Cells were then incubated with ab150117, Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) at 1/1000 dilution (shown in green) and ab150084, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 594) at 1/1000 dilution (shown in pseudocolor red). Nuclear DNA was labelled with DAPI (shown in blue).
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
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![Western blot - Anti-CTNNA1 antibody [1G5] (ab231306)](https://www.abcam.com/ps/products/231/ab231306/Images/ab231306-326819-anti-alpha-1-catenin-antibody-1g5-western-blot.jpg)
Western blot - Anti-CTNNA1 antibody [1G5] (ab231306)All lanes :
Lane 1 : HAP1 whole cell lysate
Lane 2 : HAP1 CTNNA1 KO whole cell lysate
Lane 3 : Rat colon whole tissue lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 100 kDaThis data was developed using the same antibody clone in a different buffer formulation (ab231306).
ab231306 was shown to specifically react with CTNNA1 (α-catenin) in wild type HAP1 cells. No band was observed when CTNNA1 (α-catenin) knockout samples were used. Wild-type and CTNNA1 (α-catenin) knockout samples were subjected to SDS-PAGE. ab231306 and ab181602 (Rabbit anti-GAPDH loading control) were incubated overnight at 4°C at a 1ug/ml concentraion and 1/10000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
Datasheets and documents
References
This product has been referenced in:
- Nieman MT et al. Mechanism of extracellular domain-deleted dominant negative cadherins. J Cell Sci 112 ( Pt 10):1621-32 (1999). WB ; Human . Read more (PubMed: 10212155) »
- Johnson KR et al. P- and E-cadherin are in separate complexes in cells expressing both cadherins. Exp Cell Res 207:252-60 (1993). Read more (PubMed: 8344378) »
