Key features and details
- Mouse monoclonal [1G5] to CTNNA1
- Suitable for: WB, ICC/IF
- Knockout validated
- Reacts with: Rat, Human
- Isotype: IgG1
Product nameAnti-CTNNA1 antibody [1G5]
See all CTNNA1 primary antibodies
DescriptionMouse monoclonal [1G5] to CTNNA1
Tested applicationsSuitable for: WB, ICC/IFmore details
Species reactivityReacts with: Rat, Human
Recombinant full length protein corresponding to Human CTNNA1. (A recombinant maltose binding protein fused to full length human alpha catenin).
- ICC/IF: A431 cells WB: HAP1, rat colon
This antibody clone is manufactured by Abcam. If you require it in a particular buffer formulation or a particular conjugate for your experiments, please contact email@example.com.
This product was previously labelled as alpha 1 Catenin
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.02% Sodium azide
Concentration information loading...
PurityProtein G purified
Light chain typekappa
Our Abpromise guarantee covers the use of ab231306 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Predicted molecular weight: 100 kDa.|
|ICC/IF||Use a concentration of 5 µg/ml.
This antibody gives positive signal in 4% PFA and 100% MeOH-fixed cells.
FunctionAssociates with the cytoplasmic domain of a variety of cadherins. The association of catenins to cadherins produces a complex which is linked to the actin filament network, and which seems to be of primary importance for cadherins cell-adhesion properties. Can associate with both E- and N-cadherins. Originally believed to be a stable component of E-cadherin/catenin adhesion complexes and to mediate the linkage of cadherins to the actin cytoskeleton at adherens junctions. In contrast, cortical actin was found to be much more dynamic than E-cadherin/catenin complexes and CTNNA1 was shown not to bind to F-actin when assembled in the complex suggesting a different linkage between actin and adherens junctions components. The homodimeric form may regulate actin filament assembly and inhibit actin branching by competing with the Arp2/3 complex for binding to actin filaments. May play a crucial role in cell differentiation.
Tissue specificityExpressed ubiquitously in normal tissues.
Sequence similaritiesBelongs to the vinculin/alpha-catenin family.
Cellular localizationCell membrane and Cytoplasm > cytoskeleton. Cell junction > adherens junction. Cell membrane. Cell junction. Found at cell-cell boundaries and probably at cell-matrix boundaries.
- Information by UniProt
- Alpha E-catenin antibody
- Cadherin associated protein 102kDa antibody
- Cadherin associated protein antibody
ab231306 staining CTNNA1 in A431 cells. The cells were fixed with 100% methanol (5min), permeabilized with 0.1%PBS-Tween for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab231306 at 5μg/ml and ab6046, Rabbit polyclonal to beta Tubulin - Loading Control, at 1/1000 dilution. Cells were then incubated with ab150117, Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) at 1/1000 dilution (shown in green) and ab150084, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 594) at 1/1000 dilution (shown in pseudocolor red). Nuclear DNA was labelled with DAPI (shown in blue).
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
All lanes :
Lane 1 : HAP1 whole cell lysate
Lane 2 : HAP1 CTNNA1 KO whole cell lysate
Lane 3 : Rat colon whole tissue lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 100 kDa
This data was developed using the same antibody clone in a different buffer formulation (ab231306).
ab231306 was shown to specifically react with CTNNA1 (α-catenin) in wild type HAP1 cells. No band was observed when CTNNA1 (α-catenin) knockout samples were used. Wild-type and CTNNA1 (α-catenin) knockout samples were subjected to SDS-PAGE. ab231306 and ab181602 (Rabbit anti-GAPDH loading control) were incubated overnight at 4°C at a 1ug/ml concentraion and 1/10000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab231306 has been referenced in 2 publications.
- Nieman MT et al. Mechanism of extracellular domain-deleted dominant negative cadherins. J Cell Sci 112 ( Pt 10):1621-32 (1999). WB ; Human . PubMed: 10212155
- Johnson KR et al. P- and E-cadherin are in separate complexes in cells expressing both cadherins. Exp Cell Res 207:252-60 (1993). PubMed: 8344378