Anti-CTNNA1 antibody [1G5] (ab231306)
![Immunocytochemistry/ Immunofluorescence - Anti-CTNNA1 antibody [1G5] (ab231306)](https://www.abcam.com/ps/products/231/ab231306/Images/ab231306-324645-anti-alpha-1-catenin-antibody-1g5-immunofluorescence.jpg "Immunocytochemistry/ Immunofluorescence - Anti-CTNNA1 antibody [1G5] (ab231306)")
Key features and details
- Mouse monoclonal [1G5] to CTNNA1
- Suitable for: WB, ICC/IF
- Knockout validated
- Reacts with: Rat, Human
- Isotype: IgG1
Overview
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Product name
Anti-CTNNA1 antibody [1G5]
See all CTNNA1 primary antibodies -
Description
Mouse monoclonal [1G5] to CTNNA1 -
Host species
Mouse -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Rat, Human -
Immunogen
Recombinant full length protein corresponding to Human CTNNA1. (A recombinant maltose binding protein fused to full length human alpha catenin).
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Positive control
- ICC/IF: A431 cells WB: HAP1, rat colon
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General notes
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
1G5 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab231306 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
Use a concentration of 1 µg/ml. Predicted molecular weight: 100 kDa.
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| ICC/IF |
Use a concentration of 5 µg/ml.
This antibody gives positive signal in 4% PFA and 100% MeOH-fixed cells. |
| Notes |
|---|
|
WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 100 kDa. |
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ICC/IF
Use a concentration of 5 µg/ml. This antibody gives positive signal in 4% PFA and 100% MeOH-fixed cells. |
Target
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Function
Associates with the cytoplasmic domain of a variety of cadherins. The association of catenins to cadherins produces a complex which is linked to the actin filament network, and which seems to be of primary importance for cadherins cell-adhesion properties. Can associate with both E- and N-cadherins. Originally believed to be a stable component of E-cadherin/catenin adhesion complexes and to mediate the linkage of cadherins to the actin cytoskeleton at adherens junctions. In contrast, cortical actin was found to be much more dynamic than E-cadherin/catenin complexes and CTNNA1 was shown not to bind to F-actin when assembled in the complex suggesting a different linkage between actin and adherens junctions components. The homodimeric form may regulate actin filament assembly and inhibit actin branching by competing with the Arp2/3 complex for binding to actin filaments. May play a crucial role in cell differentiation. -
Tissue specificity
Expressed ubiquitously in normal tissues. -
Sequence similarities
Belongs to the vinculin/alpha-catenin family. -
Post-translational
modificationsSumoylated. -
Cellular localization
Cell membrane and Cytoplasm > cytoskeleton. Cell junction > adherens junction. Cell membrane. Cell junction. Found at cell-cell boundaries and probably at cell-matrix boundaries. - Information by UniProt
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Database links
- Entrez Gene: 1495 Human
- Entrez Gene: 307505 Rat
- Omim: 116805 Human
- SwissProt: P35221 Human
- Unigene: 445981 Human
- Unigene: 656653 Human
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Alternative names
- Alpha E-catenin antibody
- Cadherin associated protein 102kDa antibody
- Cadherin associated protein antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence - Anti-CTNNA1 antibody [1G5] (ab231306)
ab231306 staining CTNNA1 in A431 cells. The cells were fixed with 100% methanol (5min), permeabilized with 0.1%PBS-Tween for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab231306 at 5μg/ml and ab6046, Rabbit polyclonal to beta Tubulin - Loading Control, at 1/1000 dilution. Cells were then incubated with ab150117, Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) at 1/1000 dilution (shown in green) and ab150084, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 594) at 1/1000 dilution (shown in pseudocolor red). Nuclear DNA was labelled with DAPI (shown in blue).
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
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![Western blot - Anti-CTNNA1 antibody [1G5] (ab231306)](https://www.abcam.com/ps/products/231/ab231306/Images/ab231306-326819-anti-alpha-1-catenin-antibody-1g5-western-blot.jpg)
Western blot - Anti-CTNNA1 antibody [1G5] (ab231306)All lanes :
Lane 1 : HAP1 whole cell lysate
Lane 2 : HAP1 CTNNA1 KO whole cell lysate
Lane 3 : Rat colon whole tissue lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 100 kDaThis data was developed using the same antibody clone in a different buffer formulation (ab231306).
ab231306 was shown to specifically react with CTNNA1 (α-catenin) in wild type HAP1 cells. No band was observed when CTNNA1 (α-catenin) knockout samples were used. Wild-type and CTNNA1 (α-catenin) knockout samples were subjected to SDS-PAGE. ab231306 and ab181602 (Rabbit anti-GAPDH loading control) were incubated overnight at 4°C at a 1ug/ml concentraion and 1/10000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (3)
ab231306 has been referenced in 3 publications.
- Pence LJ et al. PLEKHA7, an Apical Adherens Junction Protein, Suppresses Inflammatory Breast Cancer in the Context of High E-Cadherin and p120-Catenin Expression. Int J Mol Sci 22:N/A (2021). PubMed: 33525380
- Nieman MT et al. Mechanism of extracellular domain-deleted dominant negative cadherins. J Cell Sci 112 ( Pt 10):1621-32 (1999). WB ; Human . PubMed: 10212155
- Johnson KR et al. P- and E-cadherin are in separate complexes in cells expressing both cadherins. Exp Cell Res 207:252-60 (1993). PubMed: 8344378
