Overview

  • Product name

  • Description

    Rabbit polyclonal to CTNNA1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WB, IHC-Fr, IHC - Wholemountmore details
  • Species reactivity

    Reacts with: Zebrafish
  • Immunogen

    Recombinant fragment within Zebrafish CTNNA1 aa 60-344. The exact sequence is proprietary.
    Database link: 30741

  • Positive control

    • Zebrafish muscle whole cell extract; Zebrafish tissue; Zebrafish retina and eye tissues.
  • General notes

     This product was previously labelled as alpha 1 Catenin

     

Properties

Applications

Our Abpromise guarantee covers the use of ab210097 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Suggested antigen retrieval using 1mM EDTA-NaOH (pH8.0) 95°C, 1Hour.

WB 1/500 - 1/3000. Predicted molecular weight: 100 kDa.
IHC-Fr 1/100 - 1/1000.

Suggested antigen retrieval using sodium citrate buffer (pH6.0) 98°C, 5 minutes or 2N HCl in PBS/0.5% triton X-100, 30 minutes at room temperature.

IHC - Wholemount 1/100 - 1/500.

Target

  • Function

    Associates with the cytoplasmic domain of a variety of cadherins. The association of catenins to cadherins produces a complex which is linked to the actin filament network, and which seems to be of primary importance for cadherins cell-adhesion properties. Can associate with both E- and N-cadherins. Originally believed to be a stable component of E-cadherin/catenin adhesion complexes and to mediate the linkage of cadherins to the actin cytoskeleton at adherens junctions. In contrast, cortical actin was found to be much more dynamic than E-cadherin/catenin complexes and CTNNA1 was shown not to bind to F-actin when assembled in the complex suggesting a different linkage between actin and adherens junctions components. The homodimeric form may regulate actin filament assembly and inhibit actin branching by competing with the Arp2/3 complex for binding to actin filaments. May play a crucial role in cell differentiation.
  • Tissue specificity

    Expressed ubiquitously in normal tissues.
  • Sequence similarities

    Belongs to the vinculin/alpha-catenin family.
  • Post-translational
    modifications

    Sumoylated.
  • Cellular localization

    Cell membrane and Cytoplasm > cytoskeleton. Cell junction > adherens junction. Cell membrane. Cell junction. Found at cell-cell boundaries and probably at cell-matrix boundaries.
  • Information by UniProt
  • Database links

    • Alternative names

      • Alpha E-catenin antibody
      • Cadherin associated protein 102kDa antibody
      • Cadherin associated protein antibody
      • Cadherin-associated protein antibody
      • CAP 102 antibody
      • CAP102 antibody
      • Catenin (cadherin associated protein) alpha 1 102kDa antibody
      • Catenin (cadherin associated protein), alpha 1, 102kDa antibody
      • Catenin alpha 1 antibody
      • Catenin alpha-1 antibody
      • CTNA1_HUMAN antibody
      • CTNNA 1 antibody
      • Ctnna1 antibody
      • FLJ36832 antibody
      • FLJ52416 antibody
      • MDPT2 antibody
      • NY REN 13 antigen antibody
      • OTTHUMP00000224141 antibody
      • OTTHUMP00000224147 antibody
      • Renal carcinoma antigen NY REN 13 antibody
      • Renal carcinoma antigen NY-REN-13 antibody
      see all

    Images

    • Anti-CTNNA1 antibody (ab210097) at 1/500 dilution + Zebrafish muscle whole cell extract at 30 µg

      Predicted band size: 100 kDa



      7.5% SDS-PAGE

    • Immunohistochemical analysis of frozen sections of Zebrafish retina tissue, labeling CTNNA1 using ab210097 at 1/500 dilution.

    • Immunohistochemical analysis of frozen sections of Zebrafish retina tissue, labeling CTNNA1 using ab210097 at 1/1000 dilution.

    • Immunohistochemical analysis of paraffin embedded Zebrafish tissue, labeling CTNNA1 using ab210097 at 1/300 dilution.

    • Immunohistochemical wholemount analysis of Zebrafish embryo eye section labeling CTNNA1 (green) with ab210097 at 1/200 dilution.

    References

    ab210097 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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