Anti-CTNNA1 antibody (ab227181)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-CTNNA1 antibody
See all CTNNA1 primary antibodies -
Description
Rabbit polyclonal to CTNNA1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IF, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Rabbit, Cow, Xenopus laevis, Rhesus monkey
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Immunogen
Recombinant fragment within Human CTNNA1 (internal sequence). The exact sequence is proprietary.
Database link: P35221 -
Positive control
- WB: HepG2, NIH/3T3, JC and PC-12 whole cell lysates. IHC-P: Mouse prostate and intestine tissue. ICC/IF: HeLa cells.
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General notes
This product was previously labelled as alpha 1 Catenin
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
Our Abpromise guarantee covers the use of ab227181 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/10000. Predicted molecular weight: 100 kDa. | |
| ICC/IF | 1/100 - 1/1000. | |
| IHC-P | 1/100 - 1/1000. |
Target
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Function
Associates with the cytoplasmic domain of a variety of cadherins. The association of catenins to cadherins produces a complex which is linked to the actin filament network, and which seems to be of primary importance for cadherins cell-adhesion properties. Can associate with both E- and N-cadherins. Originally believed to be a stable component of E-cadherin/catenin adhesion complexes and to mediate the linkage of cadherins to the actin cytoskeleton at adherens junctions. In contrast, cortical actin was found to be much more dynamic than E-cadherin/catenin complexes and CTNNA1 was shown not to bind to F-actin when assembled in the complex suggesting a different linkage between actin and adherens junctions components. The homodimeric form may regulate actin filament assembly and inhibit actin branching by competing with the Arp2/3 complex for binding to actin filaments. May play a crucial role in cell differentiation. -
Tissue specificity
Expressed ubiquitously in normal tissues. -
Sequence similarities
Belongs to the vinculin/alpha-catenin family. -
Post-translational
modificationsSumoylated. -
Cellular localization
Cell membrane and Cytoplasm > cytoskeleton. Cell junction > adherens junction. Cell membrane. Cell junction. Found at cell-cell boundaries and probably at cell-matrix boundaries. - Information by UniProt
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Database links
- Entrez Gene: 1495 Human
- Entrez Gene: 12385 Mouse
- Entrez Gene: 307505 Rat
- Entrez Gene: 399303 Xenopus laevis
- Omim: 116805 Human
- SwissProt: P35221 Human
- SwissProt: P26231 Mouse
- Unigene: 445981 Human
see all -
Alternative names
- Alpha E-catenin antibody
- Cadherin associated protein 102kDa antibody
- Cadherin associated protein antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CTNNA1 antibody (ab227181)Paraffin-embedded mouse intestine tissue stained for CTNNA1 using ab227181 at 1/500 dilution in immunohistochemical analysis.
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Western blot - Anti-CTNNA1 antibody (ab227181)All lanes : Anti-CTNNA1 antibody (ab227181) at 1/1000 dilution
Lane 1 : 293T cell lysate
Lane 2 : A431 cell lysate
Lane 3 : HeLa cell lysate
Lane 4 : HepG2 cell lysate
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 100 kDa -
Immunocytochemistry/ Immunofluorescence - Anti-CTNNA1 antibody (ab227181)4% paraformaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for CTNNA1 (green) using ab227181 at 1/500 dilution in ICC/IF.
Nuclear Counterstain: Hoechst 33342 (blue).
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Western blot - Anti-CTNNA1 antibody (ab227181)All lanes : Anti-CTNNA1 antibody (ab227181) at 1/10000 dilution
Lane 1 : NIH/3T3 (mouse embryonic fibroblast cell line) whole cell lysate
Lane 2 : JC whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 100 kDa7.5% SDS-PAGE gel.
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Western blot - Anti-CTNNA1 antibody (ab227181)Anti-CTNNA1 antibody (ab227181) at 1/10000 dilution + PC-12 (rat adrenal gland pheochromocytoma cell line) whole cell lysate at 30 µg
Predicted band size: 100 kDa7.5% SDS-PAGE gel.
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Western blot - Anti-CTNNA1 antibody (ab227181)Anti-CTNNA1 antibody (ab227181) at 1/1000 dilution + HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate at 30 µg
Predicted band size: 100 kDa7.5% SDS-PAGE gel.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CTNNA1 antibody (ab227181)Paraffin-embedded mouse prostate carcinoma tissue stained for CTNNA1 using ab227181 at 1/500 dilution in immunohistochemical analysis.
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Immunocytochemistry/ Immunofluorescence - Anti-CTNNA1 antibody (ab227181)4% paraformaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for CTNNA1 (green) using ab227181 at 1/500 dilution in ICC/IF.
Counterstain: alpha Tubulin was stained by an alpha Tubulin antibody at 1/500 dilution (red). Nuclear Counterstain: Hoechst 33342 (blue).
References
ab227181 has not yet been referenced specifically in any publications.
