Overview

  • Product name

  • Description

    Rabbit polyclonal to CTNNA1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rabbit, Cow
  • Immunogen

    Recombinant fragment (His-T7-tag) corresponding to Human CTNNA1 aa 2-228. Expressed in E. coli. N-terminal tags.
    Sequence:

    TAVHAGNIN FKWDPKSLEI RTLAVERLLE PLVTQVTTLV NTNSKGPSNK KRGRSKKAHV LAASVEQATE NFLEKGDKIA KESQFLKEEL VAAVEDVRKQ GDLMKAAAGE FADDPCSSVK RGNMVRAARA LLSAVTRLLI LADMADVYKL LVQLKVVEDG ILKLRNAGNE QDLGIQYKAL KPEVDKLNIMAAKRQQELKD VGHRDQMAAARGILQKNVPI LYTASQAC


    Database link: P35221

  • Positive control

    • IHC-P: Human stomach, liver and kidney tissues. WB: Recombinant human CTNNA1 protein; HeLa and A431 cell lysate; Pig brain tissue lysate. ICC/IF: HeLa cells.
  • General notes

     This product was previously labelled as alpha 1 Catenin

     

Properties

Applications

Our Abpromise guarantee covers the use of ab231281 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 5 - 20 µg/ml.
WB Use a concentration of 0.5 - 2 µg/ml.
ICC/IF Use a concentration of 5 - 20 µg/ml.

Target

  • Function

    Associates with the cytoplasmic domain of a variety of cadherins. The association of catenins to cadherins produces a complex which is linked to the actin filament network, and which seems to be of primary importance for cadherins cell-adhesion properties. Can associate with both E- and N-cadherins. Originally believed to be a stable component of E-cadherin/catenin adhesion complexes and to mediate the linkage of cadherins to the actin cytoskeleton at adherens junctions. In contrast, cortical actin was found to be much more dynamic than E-cadherin/catenin complexes and CTNNA1 was shown not to bind to F-actin when assembled in the complex suggesting a different linkage between actin and adherens junctions components. The homodimeric form may regulate actin filament assembly and inhibit actin branching by competing with the Arp2/3 complex for binding to actin filaments. May play a crucial role in cell differentiation.
  • Tissue specificity

    Expressed ubiquitously in normal tissues.
  • Sequence similarities

    Belongs to the vinculin/alpha-catenin family.
  • Post-translational
    modifications

    Sumoylated.
  • Cellular localization

    Cell membrane and Cytoplasm > cytoskeleton. Cell junction > adherens junction. Cell membrane. Cell junction. Found at cell-cell boundaries and probably at cell-matrix boundaries.
  • Information by UniProt
  • Database links

  • Alternative names

    • Alpha E-catenin antibody
    • Cadherin associated protein 102kDa antibody
    • Cadherin associated protein antibody
    • Cadherin-associated protein antibody
    • CAP 102 antibody
    • CAP102 antibody
    • Catenin (cadherin associated protein) alpha 1 102kDa antibody
    • Catenin (cadherin associated protein), alpha 1, 102kDa antibody
    • Catenin alpha 1 antibody
    • Catenin alpha-1 antibody
    • CTNA1_HUMAN antibody
    • CTNNA 1 antibody
    • Ctnna1 antibody
    • FLJ36832 antibody
    • FLJ52416 antibody
    • MDPT2 antibody
    • NY REN 13 antigen antibody
    • OTTHUMP00000224141 antibody
    • OTTHUMP00000224147 antibody
    • Renal carcinoma antigen NY REN 13 antibody
    • Renal carcinoma antigen NY-REN-13 antibody
    see all

Images

  • All lanes : Anti-CTNNA1 antibody (ab231281) at 2 µg/ml

    Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
    Lane 2 : Pig brain tissue lysate
    Lane 3 : A431 (human epidermoid carcinoma cell line) whole cell lysate
  • Formalin-fixed, paraffin-embedded human stomach tissue stained for CTNNA1 using ab231281 at 20 µg/mL in immunohistochemical analysis. DAB staining.

  • HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for CTNNA1 using ab231281 at 20 µg/mL in ICC/IF. FITC secondary used.

  • Formalin-fixed, paraffin-embedded human kidney tissue stained for CTNNA1 using ab231281 at 20 µg/mL in immunohistochemical analysis. DAB staining.

  • Formalin-fixed, paraffin-embedded human liver tissue stained for CTNNA1 using ab231281 at 20 µg/mL in immunohistochemical analysis. DAB staining.

  • Anti-CTNNA1 antibody (ab231281) at 2 µg/ml + Recombinant human CTNNA1 protein

References

ab231281 has not yet been referenced specifically in any publications.

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