Key features and details
- Rabbit polyclonal to CTNNA1
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-CTNNA1 antibody
See all CTNNA1 primary antibodies
DescriptionRabbit polyclonal to CTNNA1
Tested applicationsSuitable for: IHC-P, WB, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rabbit, Cow
Recombinant fragment (His-T7-tag) corresponding to Human CTNNA1 aa 2-228. Expressed in E. coli. N-terminal tags.
TAVHAGNIN FKWDPKSLEI RTLAVERLLE PLVTQVTTLV NTNSKGPSNK KRGRSKKAHV LAASVEQATE NFLEKGDKIA KESQFLKEEL VAAVEDVRKQ GDLMKAAAGE FADDPCSSVK RGNMVRAARA LLSAVTRLLI LADMADVYKL LVQLKVVEDG ILKLRNAGNE QDLGIQYKAL KPEVDKLNIMAAKRQQELKD VGHRDQMAAARGILQKNVPI LYTASQAC
Database link: P35221
- IHC-P: Human stomach, liver and kidney tissues. WB: Recombinant human CTNNA1 protein; HeLa and A431 cell lysate; Pig brain tissue lysate. ICC/IF: HeLa cells.
This product was previously labelled as alpha 1 Catenin
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol
Concentration information loading...
PurityProtein A purified
Purification notesAntigen-specific affinity chromatography followed by Protein A affinity chromatography.
Our Abpromise guarantee covers the use of ab231281 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 5 - 20 µg/ml.|
|WB||Use a concentration of 0.5 - 2 µg/ml.|
|ICC/IF||Use a concentration of 5 - 20 µg/ml.|
FunctionAssociates with the cytoplasmic domain of a variety of cadherins. The association of catenins to cadherins produces a complex which is linked to the actin filament network, and which seems to be of primary importance for cadherins cell-adhesion properties. Can associate with both E- and N-cadherins. Originally believed to be a stable component of E-cadherin/catenin adhesion complexes and to mediate the linkage of cadherins to the actin cytoskeleton at adherens junctions. In contrast, cortical actin was found to be much more dynamic than E-cadherin/catenin complexes and CTNNA1 was shown not to bind to F-actin when assembled in the complex suggesting a different linkage between actin and adherens junctions components. The homodimeric form may regulate actin filament assembly and inhibit actin branching by competing with the Arp2/3 complex for binding to actin filaments. May play a crucial role in cell differentiation.
Tissue specificityExpressed ubiquitously in normal tissues.
Sequence similaritiesBelongs to the vinculin/alpha-catenin family.
Cellular localizationCell membrane and Cytoplasm > cytoskeleton. Cell junction > adherens junction. Cell membrane. Cell junction. Found at cell-cell boundaries and probably at cell-matrix boundaries.
- Information by UniProt
- Alpha E-catenin antibody
- Cadherin associated protein 102kDa antibody
- Cadherin associated protein antibody
All lanes : Anti-CTNNA1 antibody (ab231281) at 2 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : Pig brain tissue lysate
Lane 3 : A431 (human epidermoid carcinoma cell line) whole cell lysate
Formalin-fixed, paraffin-embedded human stomach tissue stained for CTNNA1 using ab231281 at 20 µg/mL in immunohistochemical analysis. DAB staining.
HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for CTNNA1 using ab231281 at 20 µg/mL in ICC/IF. FITC secondary used.
Formalin-fixed, paraffin-embedded human kidney tissue stained for CTNNA1 using ab231281 at 20 µg/mL in immunohistochemical analysis. DAB staining.
Formalin-fixed, paraffin-embedded human liver tissue stained for CTNNA1 using ab231281 at 20 µg/mL in immunohistochemical analysis. DAB staining.
Anti-CTNNA1 antibody (ab231281) at 2 µg/ml + Recombinant human CTNNA1 protein
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab231281 has not yet been referenced specifically in any publications.