Recombinant Anti-CTNNA1 antibody [EP1793Y] - Low endotoxin, Azide free (ab226010)
![Immunoprecipitation - Anti-CTNNA1 antibody [EP1793Y] - Low endotoxin, Azide free (ab226010)](https://www.abcam.com/ps/products/226/ab226010/Images/ab226010-414394-AntiCTNNA1-antibody.jpg "Immunoprecipitation - Anti-CTNNA1 antibody [EP1793Y] - Low endotoxin, Azide free (ab226010)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP1793Y] to CTNNA1 - Low endotoxin, Azide free
- Suitable for: IHC-P, WB, IP
- Knockout validated
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-CTNNA1 antibody [EP1793Y] - Low endotoxin, Azide free
See all CTNNA1 primary antibodies -
Description
Rabbit monoclonal [EP1793Y] to CTNNA1 - Low endotoxin, Azide free -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, IPmore details
Unsuitable for: Flow Cyt or ICC/IF -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Mouse heart and kidney lysate. Rat brain and kidney lysate. HeLa, A431 and HUVEC whole cell lysate. IHC-P: Rat and human stomach tissue and mouse liver tissue IP: HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate
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General notes
ab226010 is the carrier-free version of ab51032.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Our Low endotoxin, azide-free formats have low endotoxin level (≤ 1 EU/ml, determined by the LAL assay) and are free from azide, to achieve consistent experimental results in functional assays.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.20
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EP1793Y -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab226010 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| WB |
Use at an assay dependent concentration. Detects a band of approximately 100 kDa (predicted molecular weight: 100 kDa).
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| IP |
Use at an assay dependent concentration.
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| Notes |
|---|
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IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
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WB
Use at an assay dependent concentration. Detects a band of approximately 100 kDa (predicted molecular weight: 100 kDa). |
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IP
Use at an assay dependent concentration. |
Target
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Function
Associates with the cytoplasmic domain of a variety of cadherins. The association of catenins to cadherins produces a complex which is linked to the actin filament network, and which seems to be of primary importance for cadherins cell-adhesion properties. Can associate with both E- and N-cadherins. Originally believed to be a stable component of E-cadherin/catenin adhesion complexes and to mediate the linkage of cadherins to the actin cytoskeleton at adherens junctions. In contrast, cortical actin was found to be much more dynamic than E-cadherin/catenin complexes and CTNNA1 was shown not to bind to F-actin when assembled in the complex suggesting a different linkage between actin and adherens junctions components. The homodimeric form may regulate actin filament assembly and inhibit actin branching by competing with the Arp2/3 complex for binding to actin filaments. May play a crucial role in cell differentiation. -
Tissue specificity
Expressed ubiquitously in normal tissues. -
Sequence similarities
Belongs to the vinculin/alpha-catenin family. -
Post-translational
modificationsSumoylated. -
Cellular localization
Cell membrane and Cytoplasm > cytoskeleton. Cell junction > adherens junction. Cell membrane. Cell junction. Found at cell-cell boundaries and probably at cell-matrix boundaries. - Information by UniProt
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Database links
- Entrez Gene: 1495 Human
- Entrez Gene: 12385 Mouse
- Entrez Gene: 307505 Rat
- Omim: 116805 Human
- SwissProt: P35221 Human
- SwissProt: P26231 Mouse
- Unigene: 445981 Human
- Unigene: 656653 Human
see all -
Alternative names
- Alpha E-catenin antibody
- Cadherin associated protein 102kDa antibody
- Cadherin associated protein antibody
see all
Images
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Immunoprecipitation - Anti-CTNNA1 antibody [EP1793Y] - Low endotoxin, Azide free (ab226010)
ab190685 at 1/100 dilution immunoprecipitating CTNNA1 in Jurkat HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysates
Lane 1 (input): HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate 10μg
Lane 2 (+): HeLa whole cell lysate
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab51032 in HeLa whole cell lysate
For western blotting, ab51032 at 1/500 dilution and VeriBlot for IP Detection Reagent (HRP)(ab131366) at 1/1000 dilution were used.Blocking and diluting buffer: 5% NFDM /TBST.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab51032).
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CTNNA1 antibody [EP1793Y] - Low endotoxin, Azide free (ab226010)](https://www.abcam.com/ps/products/226/ab226010/Images/ab226010-414392-AntiCTNNA1-antibody.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CTNNA1 antibody [EP1793Y] - Low endotoxin, Azide free (ab226010)Paraffin-embedded rat stomach tissue stained for CTNNA1 with ab51032 at a 1/100 dilution in immunohistochemical analysis. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as a secondary antibody and Hematoxylin used as a counterstain. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) was performed for 20 minutes.
Positive staining was seen on rat stomach.
The section was incubated with ab51032 for 30 minutes at room temperature.
The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab51032).
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CTNNA1 antibody [EP1793Y] - Low endotoxin, Azide free (ab226010)](https://www.abcam.com/ps/products/226/ab226010/Images/ab226010-414390-AntiCTNNA1-antibody.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CTNNA1 antibody [EP1793Y] - Low endotoxin, Azide free (ab226010)Paraffin-embedded mouse liver tissue stained for CTNNA1 with ab51032 at a 1/100 dilution in immunohistochemical analysis. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as a secondary antibody and Hematoxylin used as a counterstain. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) was performed for 20 minutes.
Positive staining was seen on mouse liver.
The section was incubated with ab51032 for 30 minutes at room temperature.
The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab51032).
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CTNNA1 antibody [EP1793Y] - Low endotoxin, Azide free (ab226010)](https://www.abcam.com/ps/products/226/ab226010/Images/ab226010-414388-AntiCTNNA1-antibody.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CTNNA1 antibody [EP1793Y] - Low endotoxin, Azide free (ab226010)Paraffin-embedded human stomach tissue stained for CTNNA1 with ab51032 at a 1/100 dilution in immunohistochemical analysis. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as a secondary antibody and Hematoxylin used as a counterstain. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) was performed for 20 minutes.
Positive staining was seen on human stomach.
The section was incubated with ab51032 for 30 minutes at room temperature.
The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab51032).
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![Western blot - Anti-CTNNA1 antibody [EP1793Y] - Low endotoxin, Azide free (ab226010)](https://www.abcam.com/ps/products/226/ab226010/Images/ab226010-414386-AntiCTNNA1-antibody.jpg)
Western blot - Anti-CTNNA1 antibody [EP1793Y] - Low endotoxin, Azide free (ab226010)All lanes : Anti-CTNNA1 antibody [EP1793Y] (ab51032) at 1/10000 dilution
Lane 1 : Mouse heart lysate
Lane 2 : Mouse kidney lysate
Lane 3 : Rat brain lysate
Lane 4 : Rat kidney lysate
Lane 5 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate
Lane 6 : A431 (Human epidermoid carcinoma epithelial cell) whole cell lysate
Lane 7 : HUVEC (Human umbilical vein endothelial cell) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051)
Predicted band size: 100 kDa
Observed band size: 100 kDaExpsure times
Lane 1-4: 180 seconds
Lane 5,7: 40 seconds
Lane 6: 5 seconds
Blocking/diluting buffer and concentration: 5% NFDM/TBST
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab51032).
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![Western blot - Anti-CTNNA1 antibody [EP1793Y] - Low endotoxin, Azide free (ab226010)](https://www.abcam.com/ps/products/226/ab226010/Images/ab226010-296607-anti-alpha-1-catenin-antibody-ep1793y-western-blot.jpg)
Western blot - Anti-CTNNA1 antibody [EP1793Y] - Low endotoxin, Azide free (ab226010)This WB data was generated using the same anti-CTNNA1 antibody clone [EP1793Y] in a different buffer formulation (cat# ab51032).
Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
Lane 2: CTNNA1 HAP1 whole cell lysate (20 µg)
Lane 3: HeLa whole cell lysate (20 µg)
Lane 4: HEK293 whole cell lysate (20 µg)Lanes 1 - 4: Merged signal (red and green). Green - ab51032 observed at 100 kDa. Red - loading control, ab9484, observed at 37 kDa.
ab51032 was shown to recognize CTNNA1 in wild-type cells as signal was lost at the expected MW in CTNNA1 knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and CTNNA1 knockout samples were subjected to SDS-PAGE. Ab51032 and ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/50000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
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![Anti-CTNNA1 antibody [EP1793Y] - Low endotoxin, Azide free (ab226010)](https://www.abcam.com/ps/products/226/ab226010/Images/ab226010-11-benefits-of-recombinant-antibodies.png)
Anti-CTNNA1 antibody [EP1793Y] - Low endotoxin, Azide free (ab226010)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (11)
ab226010 has been referenced in 11 publications.
- Qiao B et al. MicroRNA-27a-3p Modulates the Wnt/ß-Catenin Signaling Pathway to Promote Epithelial-Mesenchymal Transition in Oral Squamous Carcinoma Stem Cells by Targeting SFRP1. Sci Rep 7:44688 (2017). WB ; Human . PubMed: 28425477
- Qi L et al. TRIM16 suppresses the progression of prostate tumors by inhibiting the Snail signaling pathway. Int J Mol Med 38:1734-1742 (2016). WB ; Human . PubMed: 27748839
- Cheng G et al. Lipopolysaccharide-induced a-catenin downregulation enhances the motility of human colorectal cancer cells in an NF-?B signaling-dependent manner. Onco Targets Ther 9:7563-7571 (2016). PubMed: 28008274
- Dietrich JE et al. Venus trap in the mouse embryo reveals distinct molecular dynamics underlying specification of first embryonic lineages. EMBO Rep 16:1005-21 (2015). PubMed: 26142281
- Giampietro C et al. The actin-binding protein EPS8 binds VE-cadherin and modulates YAP localization and signaling. J Cell Biol 211:1177-92 (2015). PubMed: 26668327
