Overview

  • Product name
    Anti-CTNNA1 antibody [EP1793Y] - Low endotoxin, Azide free
    See all CTNNA1 primary antibodies
  • Description
    Rabbit monoclonal [EP1793Y] to CTNNA1 - Low endotoxin, Azide free
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IHC-P, WB, ICC/IF, IP, Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    (N terminal).

  • Positive control
    • HeLa cell lysate, human breast carcinoma tissue.
  • General notes

    The formulation and the concentration of this product is compatible for metal-conjugation for mass cytometry (CyTOF®).

    ab226010 is a PBS only buffer version of ab51032, containing no BSA or sodium azide, ideal for antibody labeling. Please refer to ab51032 for information on protocols, dilutions, and image data.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

     This product was previously labelled as alpha 1 Catenin

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

Properties

Applications

Our Abpromise guarantee covers the use of ab226010 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Detects a band of approximately 100 kDa (predicted molecular weight: 100 kDa).
ICC/IF Use at an assay dependent concentration.
IP Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration.

ab199376 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Target

  • Function
    Associates with the cytoplasmic domain of a variety of cadherins. The association of catenins to cadherins produces a complex which is linked to the actin filament network, and which seems to be of primary importance for cadherins cell-adhesion properties. Can associate with both E- and N-cadherins. Originally believed to be a stable component of E-cadherin/catenin adhesion complexes and to mediate the linkage of cadherins to the actin cytoskeleton at adherens junctions. In contrast, cortical actin was found to be much more dynamic than E-cadherin/catenin complexes and CTNNA1 was shown not to bind to F-actin when assembled in the complex suggesting a different linkage between actin and adherens junctions components. The homodimeric form may regulate actin filament assembly and inhibit actin branching by competing with the Arp2/3 complex for binding to actin filaments. May play a crucial role in cell differentiation.
  • Tissue specificity
    Expressed ubiquitously in normal tissues.
  • Sequence similarities
    Belongs to the vinculin/alpha-catenin family.
  • Post-translational
    modifications
    Sumoylated.
  • Cellular localization
    Cell membrane and Cytoplasm > cytoskeleton. Cell junction > adherens junction. Cell membrane. Cell junction. Found at cell-cell boundaries and probably at cell-matrix boundaries.
  • Information by UniProt
  • Database links
  • Alternative names
    • Alpha E-catenin antibody
    • Cadherin associated protein 102kDa antibody
    • Cadherin associated protein antibody
    • Cadherin-associated protein antibody
    • CAP 102 antibody
    • CAP102 antibody
    • Catenin (cadherin associated protein) alpha 1 102kDa antibody
    • Catenin (cadherin associated protein), alpha 1, 102kDa antibody
    • Catenin alpha 1 antibody
    • Catenin alpha-1 antibody
    • CTNA1_HUMAN antibody
    • CTNNA 1 antibody
    • Ctnna1 antibody
    • FLJ36832 antibody
    • FLJ52416 antibody
    • MDPT2 antibody
    • NY REN 13 antigen antibody
    • OTTHUMP00000224141 antibody
    • OTTHUMP00000224147 antibody
    • Renal carcinoma antigen NY REN 13 antibody
    • Renal carcinoma antigen NY-REN-13 antibody
    see all

Images

  • This WB data was generated using the same anti-CTNNA1 antibody clone [EP1793Y] in a different buffer formulation (cat# ab51032).

    Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
    Lane 2: CTNNA1 HAP1 whole cell lysate (20 µg) 
    Lane 3: HeLa whole cell lysate (20 µg)
    Lane 4: HEK293 whole cell lysate (20 µg)

    Lanes 1 - 4: Merged signal (red and green). Green - ab51032 observed at 100 kDa. Red - loading control, ab9484, observed at 37 kDa.

    ab51032 was shown to recognize CTNNA1 in wild-type cells as signal was lost at the expected MW in CTNNA1 knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and CTNNA1 knockout samples were subjected to SDS-PAGE. Ab51032 and ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/50000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

  • ICC/IF image of anti-CTNNA1 antibody [EP1793Y] (ab51032) stained mouse ES cells. The cells were fixed in 1:1 methanol/acetone, permeabilized using 0.1% Triton X, and blocked with 1% serum in PBS for 30 minutes. The cells were then incubated with ab51032 at a 1/100 dilution for 16 hours at 4oC. The secondary antibody was a Goat Anti-Rabbit Alexa Fluor® 488 (IgG H&L;  ab150077) used at a 1/500 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab51032).

  • Paraffin-embedded human breast carcinoma tissue stained for CTNNA1 with ab51032 at a 1/100 dilution in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab51032).

  • Overlay histogram showing MCF7 (Human breast adenocarcinoma cell line) cells stained with ab51032 (red line). The cells were fixed with 4% PFA (10 minutes) and then permeabilized with 0.1% PBS-Tween for 20 minutes. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the primary antibody (ab51032, 1/100 dilution) for 30 minutes at 22ºC. The secondary antibody used was goat anti-rabbit DyLight® 488 (IgG H+L) ab96899 at 1/500 dilution for 30 minutes at 22ºC. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1 µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

    This antibody gave a positive signal in MCF7 cells fixed with 80% methanol (5 minutes)/permeabilized in 0.1% PBS-Tween used under the same conditions.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab51032).

  • This ICC/IF data was generated using the same anti-CTNNA1 antibody clone [EP1793Y] in a different buffer formulation (cat# ab51032).

    ICC/IF image of ab51032 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab51032, 1µg/ml) overnight at +4°C. The secondary antibody (green) was anti-rabbit Alexa Fluor® 488 (IgG H+L; ab150077) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References

This product has been referenced in:
  • Qiao B  et al. MicroRNA-27a-3p Modulates the Wnt/ß-Catenin Signaling Pathway to Promote Epithelial-Mesenchymal Transition in Oral Squamous Carcinoma Stem Cells by Targeting SFRP1. Sci Rep 7:44688 (2017). WB ; Human . Read more (PubMed: 28425477) »
  • Qi L  et al. TRIM16 suppresses the progression of prostate tumors by inhibiting the Snail signaling pathway. Int J Mol Med 38:1734-1742 (2016). WB ; Human . Read more (PubMed: 27748839) »
See all 11 Publications for this product

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab226010.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up