Recombinant Anti-CTNNA3 antibody [EPR18307] (ab184916)

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Western blot - Anti-CTNNA3 antibody [EPR18307] (ab184916)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR18307] to CTNNA3
Suitable for: WB, IHC-P, IP
Reacts with: Mouse, Rat, Dog, Human

Carrier Free

Product name

Anti-CTNNA3 antibody [EPR18307]
See all CTNNA3 primary antibodies
Description

Rabbit monoclonal [EPR18307] to CTNNA3
Host species

Rabbit
Tested applications

Suitable for: WB, IHC-P, IPmore details
Species reactivity

Reacts with: Mouse, Rat, Dog, Human
Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: HEK-293, C2C12, Rat1, C6, PC-12, NIH/3T3 and MDCK whole cell lysates; Human fetal brain, fetal heart and fetal kidney lysates; Mouse and rat brain and heart lysates. IHC/P: Human cardiac muscle and colon cancer tissues; Mouse and rat cardiac muscle tissues. IP: HEK293 whole cell lysate.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR18307
Isotype

IgG
Research areas

Alternative Versions
- Anti-CTNNA3 antibody [EPR18307] - BSA and Azide free (ab250780)
Compatible Secondaries
- Goat Anti-Rabbit IgG H&L (HRP) (ab97051)
Isotype control
- Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab184916 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application	Abreviews	Notes
WB		1/5000. Detects a band of approximately 100 kDa (predicted molecular weight: 100 kDa).
IHC-P		1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
IP		1/100.

Notes
WB 1/5000. Detects a band of approximately 100 kDa (predicted molecular weight: 100 kDa).
IHC-P 1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
IP 1/100.

Function

May be involved in formation of stretch-resistant cell-cell adhesion complexes.
Tissue specificity

Predominantly expressed in heart and testis. Expressed at lower levels in brain, kidney, liver and skeletal muscle.
Sequence similarities

Belongs to the vinculin/alpha-catenin family.
Cellular localization

Cytoplasm > cytoskeleton. Localizes to intercalated disks of cardiomyocytes and in peritubular myoid cells of testis, and colocalizes with CTNNA1 and CTNNA2.
Target information above from: UniProt accession Q9UI47 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
Database links
- Entrez Gene: 29119 Human
- Entrez Gene: 216033 Mouse
- Entrez Gene: 361839 Rat
- Omim: 607667 Human
- SwissProt: Q9UI47 Human
- SwissProt: Q65CL1 Mouse
- Unigene: 660362 Human
- Unigene: 444692 Mouse
Alternative names
- 4930429L08Rik antibody
- 4933408A16 antibody
- Alpha catenin like protein antibody
- Alpha T catenin antibody
- Alpha T-catenin antibody
- Cadherin associated protein antibody
- Cadherin-associated protein antibody
- Catenin alpha 3 antibody
- Catenin alpha-3 antibody
- Catna3 antibody
- CTNA3_HUMAN antibody
- CTNNA3 antibody
- MGC26194 antibody
- MGC75041 antibody
- OTTHUMP00000019684 antibody
- OTTMUSP00000028119 antibody
- OTTMUSP00000028120 antibody
- RP11-433J16.1 antibody
- VR22 antibody
see all

Western blot - Anti-CTNNA3 antibody [EPR18307] (ab184916)

All lanes : Anti-CTNNA3 antibody [EPR18307] (ab184916) at 1/50000 dilution

Lane 1 : HEK-293 (Human epithelial cells from embryonic kidney) whole cell lysate
Lane 2 : C2C12 (Mouse myoblast cell line) whole cell lysate
Lane 3 : Rat1 (Rat fibroblast cell line) whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

Predicted band size: 100 kDa
Observed band size: 100 kDa

Exposure time: 3 minutes

Blocking/Dilution buffer: 5% NFDM/TBST.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CTNNA3 antibody [EPR18307] (ab184916)

Immunohistochemical analysis of paraffin-embedded Human colon cancer tissue labeling CTNNA3 with ab184916 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Cytoplasmic and membrane staining on tumor cells of Human colonic adenocarcinoma is observed. Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CTNNA3 antibody [EPR18307] (ab184916)

Immunohistochemical analysis of paraffin-embedded Mouse cardiac muscle tissue labeling CTNNA3 with ab184916 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Intercalated disks staining on Mouse cardiac muscle is observed. Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Western blot - Anti-CTNNA3 antibody [EPR18307] (ab184916)

All lanes : Anti-CTNNA3 antibody [EPR18307] (ab184916) at 1/5000 dilution

Lane 1 : Human fetal brain lysate
Lane 2 : Human fetal heart lysate
Lane 3 : Human fetal kidney lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 100 kDa
Observed band size: 100 kDa

Exposure time: 30 seconds

Blocking/Dilution buffer: 5% NFDM/TBST.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CTNNA3 antibody [EPR18307] (ab184916)

Immunohistochemical analysis of paraffin-embedded Human cardiac muscle tissue labeling CTNNA3 with ab184916 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Intercalated disks staining of normal Human cardiac muscle is observed. Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Western blot - Anti-CTNNA3 antibody [EPR18307] (ab184916)

All lanes : Anti-CTNNA3 antibody [EPR18307] (ab184916) at 1/5000 dilution

Lane 1 : Mouse brain lysate
Lane 2 : Mouse heart lysate
Lane 3 : Rat brain lysate
Lane 4 : Rat heart lysate
Lane 5 : C6 (Rat glial tumor cells) whole cell lysate
Lane 6 : PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysate
Lane 7 : NIH/3T3 (Mouse embyro fibroblast cells) whole cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

Predicted band size: 100 kDa
Observed band size: 100 kDa

Exposure time: 1 second

Blocking/Dilution buffer: 5% NFDM/TBST.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CTNNA3 antibody [EPR18307] (ab184916)

Immunohistochemical analysis of paraffin-embedded Rat cardiac muscle tissue labeling CTNNA3 with ab184916 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Intercalated disks staining on Rat cardiac muscle is observed. Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Western blot - Anti-CTNNA3 antibody [EPR18307] (ab184916)

Anti-CTNNA3 antibody [EPR18307] (ab184916) at 1/50000 dilution + MDCK (Canine kidney cell line) whole cell lysate at 10 µg

Secondary
Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

Predicted band size: 100 kDa
Observed band size: 100 kDa

Exposure time: 1 second

Blocking/Dilution buffer: 5% NFDM/TBST.
Immunoprecipitation - Anti-CTNNA3 antibody [EPR18307] (ab184916)

CTNNA3 was immunoprecipitated from 1mg of HEK-293 (Human epithelial cells from embryonic kidney) whole cell lysate with ab184916 at 1/100 dilution. Western blot was performed from the immunoprecipitate using ab184916 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.
Lane 1: HEK-293 whole cell lysate, 10 µg (Input).
Lane 2: ab184916 IP in HEK-293 whole cell lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab184916 in HEK-293 whole cell lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 1 second
Anti-CTNNA3 antibody [EPR18307] (ab184916)

Western blot protocols
Immunoprecipitation protocols
Immunohistochemistry protocols

Click here to view the general protocols

Datasheet download

Download

Publishing research using ab184916? Please let us know so that we can cite the reference in this datasheet.

ab184916 has been referenced in 1 publication.

Ortega A et al. Intercalated disc in failing hearts from patients with dilated cardiomyopathy: Its role in the depressed left ventricular function. PLoS One 12:e0185062 (2017). PubMed: 28934278

Customer reviews and Q&As

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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Key features and details

Related conjugates and formulations

Overview

Product name

Description

Host species

Tested applications

Species reactivity

Immunogen

Positive control

General notes

Properties

Form

Storage instructions

Storage buffer

Purity

Clonality

Clone number

Isotype

Research areas

Associated products

Alternative Versions

Compatible Secondaries

Isotype control

Applications

The Abpromise guarantee

Target

Function

Tissue specificity

Sequence similarities

Cellular localization

Database links

Alternative names

Images

Protocols

Datasheets and documents

Datasheet download

Certificate of Compliance

References (1)

Customer reviews and Q&As