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    ctnna3-antibody-epr18307-ab184916.pdf

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Signal Transduction Cytoskeleton / ECM Cell Adhesion Cadherins
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RecombinantRabMAb

Recombinant Anti-CTNNA3 antibody [EPR18307] (ab184916)

  • Datasheet
  • Certificate of Compliance
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Western blot - Anti-CTNNA3 antibody [EPR18307] (ab184916)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CTNNA3 antibody [EPR18307] (ab184916)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CTNNA3 antibody [EPR18307] (ab184916)
  • Western blot - Anti-CTNNA3 antibody [EPR18307] (ab184916)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CTNNA3 antibody [EPR18307] (ab184916)
  • Western blot - Anti-CTNNA3 antibody [EPR18307] (ab184916)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CTNNA3 antibody [EPR18307] (ab184916)
  • Western blot - Anti-CTNNA3 antibody [EPR18307] (ab184916)
  • Immunoprecipitation - Anti-CTNNA3 antibody [EPR18307] (ab184916)
  • Anti-CTNNA3 antibody [EPR18307] (ab184916)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR18307] to CTNNA3
  • Suitable for: WB, IHC-P, IP
  • Reacts with: Mouse, Rat, Dog, Human

Conjugates logo Related conjugates and formulations

Carrier Free

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Anti-HOXC10 antibody (ab153904)

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Overview

  • Product name

    Anti-CTNNA3 antibody [EPR18307]
    See all CTNNA3 primary antibodies
  • Description

    Rabbit monoclonal [EPR18307] to CTNNA3
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, IPmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Dog, Human
  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HEK-293, C2C12, Rat1, C6, PC-12, NIH/3T3 and MDCK whole cell lysates; Human fetal brain, fetal heart and fetal kidney lysates; Mouse and rat brain and heart lysates. IHC/P: Human cardiac muscle and colon cancer tissues; Mouse and rat cardiac muscle tissues. IP: HEK293 whole cell lysate.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR18307
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Cytoskeleton / ECM
    • Cell Adhesion
    • Cadherins
    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Microfilaments
    • Actin etc
    • Catenins
    • Signal Transduction
    • Protein Phosphorylation
    • Tyrosine Kinases
    • Other

Associated products

  • Alternative Versions

    • Anti-CTNNA3 antibody [EPR18307] - BSA and Azide free (ab250780)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (HRP) (ab97051)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab184916 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/5000. Detects a band of approximately 100 kDa (predicted molecular weight: 100 kDa).
IHC-P
1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
IP
1/100.
Notes
WB
1/5000. Detects a band of approximately 100 kDa (predicted molecular weight: 100 kDa).
IHC-P
1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
IP
1/100.

Target

  • Function

    May be involved in formation of stretch-resistant cell-cell adhesion complexes.
  • Tissue specificity

    Predominantly expressed in heart and testis. Expressed at lower levels in brain, kidney, liver and skeletal muscle.
  • Sequence similarities

    Belongs to the vinculin/alpha-catenin family.
  • Cellular localization

    Cytoplasm > cytoskeleton. Localizes to intercalated disks of cardiomyocytes and in peritubular myoid cells of testis, and colocalizes with CTNNA1 and CTNNA2.
  • Target information above from: UniProt accession Q9UI47 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 29119 Human
    • Entrez Gene: 216033 Mouse
    • Entrez Gene: 361839 Rat
    • Omim: 607667 Human
    • SwissProt: Q9UI47 Human
    • SwissProt: Q65CL1 Mouse
    • Unigene: 660362 Human
    • Unigene: 444692 Mouse
    • Alternative names

      • 4930429L08Rik antibody
      • 4933408A16 antibody
      • Alpha catenin like protein antibody
      • Alpha T catenin antibody
      • Alpha T-catenin antibody
      • Cadherin associated protein antibody
      • Cadherin-associated protein antibody
      • Catenin alpha 3 antibody
      • Catenin alpha-3 antibody
      • Catna3 antibody
      • CTNA3_HUMAN antibody
      • CTNNA3 antibody
      • MGC26194 antibody
      • MGC75041 antibody
      • OTTHUMP00000019684 antibody
      • OTTMUSP00000028119 antibody
      • OTTMUSP00000028120 antibody
      • RP11-433J16.1 antibody
      • VR22 antibody
      see all

    Images

    • Western blot - Anti-CTNNA3 antibody [EPR18307] (ab184916)
      Western blot - Anti-CTNNA3 antibody [EPR18307] (ab184916)
      All lanes : Anti-CTNNA3 antibody [EPR18307] (ab184916) at 1/50000 dilution

      Lane 1 : HEK-293 (Human epithelial cells from embryonic kidney) whole cell lysate
      Lane 2 : C2C12 (Mouse myoblast cell line) whole cell lysate
      Lane 3 : Rat1 (Rat fibroblast cell line) whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

      Predicted band size: 100 kDa
      Observed band size: 100 kDa


      Exposure time: 3 minutes


      Blocking/Dilution buffer: 5% NFDM/TBST.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CTNNA3 antibody [EPR18307] (ab184916)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CTNNA3 antibody [EPR18307] (ab184916)

      Immunohistochemical analysis of paraffin-embedded Human colon cancer tissue labeling CTNNA3 with ab184916 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Cytoplasmic and membrane staining on tumor cells of Human colonic adenocarcinoma is observed. Counter stained with Hematoxylin.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CTNNA3 antibody [EPR18307] (ab184916)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CTNNA3 antibody [EPR18307] (ab184916)

      Immunohistochemical analysis of paraffin-embedded Mouse cardiac muscle tissue labeling CTNNA3 with ab184916 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Intercalated disks staining on Mouse cardiac muscle is observed. Counter stained with Hematoxylin.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    • Western blot - Anti-CTNNA3 antibody [EPR18307] (ab184916)
      Western blot - Anti-CTNNA3 antibody [EPR18307] (ab184916)
      All lanes : Anti-CTNNA3 antibody [EPR18307] (ab184916) at 1/5000 dilution

      Lane 1 : Human fetal brain lysate
      Lane 2 : Human fetal heart lysate
      Lane 3 : Human fetal kidney lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

      Predicted band size: 100 kDa
      Observed band size: 100 kDa


      Exposure time: 30 seconds


      Blocking/Dilution buffer: 5% NFDM/TBST.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CTNNA3 antibody [EPR18307] (ab184916)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CTNNA3 antibody [EPR18307] (ab184916)

      Immunohistochemical analysis of paraffin-embedded Human cardiac muscle tissue labeling CTNNA3 with ab184916 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Intercalated disks staining of normal Human cardiac muscle is observed. Counter stained with Hematoxylin.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    • Western blot - Anti-CTNNA3 antibody [EPR18307] (ab184916)
      Western blot - Anti-CTNNA3 antibody [EPR18307] (ab184916)
      All lanes : Anti-CTNNA3 antibody [EPR18307] (ab184916) at 1/5000 dilution

      Lane 1 : Mouse brain lysate
      Lane 2 : Mouse heart lysate
      Lane 3 : Rat brain lysate
      Lane 4 : Rat heart lysate
      Lane 5 : C6 (Rat glial tumor cells) whole cell lysate
      Lane 6 : PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysate
      Lane 7 : NIH/3T3 (Mouse embyro fibroblast cells) whole cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

      Predicted band size: 100 kDa
      Observed band size: 100 kDa


      Exposure time: 1 second


      Blocking/Dilution buffer: 5% NFDM/TBST.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CTNNA3 antibody [EPR18307] (ab184916)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CTNNA3 antibody [EPR18307] (ab184916)

      Immunohistochemical analysis of paraffin-embedded Rat cardiac muscle tissue labeling CTNNA3 with ab184916 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Intercalated disks staining on Rat cardiac muscle is observed. Counter stained with Hematoxylin.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    • Western blot - Anti-CTNNA3 antibody [EPR18307] (ab184916)
      Western blot - Anti-CTNNA3 antibody [EPR18307] (ab184916)
      Anti-CTNNA3 antibody [EPR18307] (ab184916) at 1/50000 dilution + MDCK (Canine kidney cell line) whole cell lysate at 10 µg

      Secondary
      Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

      Predicted band size: 100 kDa
      Observed band size: 100 kDa


      Exposure time: 1 second


      Blocking/Dilution buffer: 5% NFDM/TBST.

    • Immunoprecipitation - Anti-CTNNA3 antibody [EPR18307] (ab184916)
      Immunoprecipitation - Anti-CTNNA3 antibody [EPR18307] (ab184916)

      CTNNA3 was immunoprecipitated from 1mg of HEK-293 (Human epithelial cells from embryonic kidney) whole cell lysate with ab184916 at 1/100 dilution. Western blot was performed from the immunoprecipitate using ab184916 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.

      Lane 1: HEK-293 whole cell lysate, 10 µg (Input).

      Lane 2: ab184916 IP in HEK-293 whole cell lysate.

      Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab184916 in HEK-293 whole cell lysate.
      Blocking and dilution buffer and concentration: 5% NFDM/TBST.

      Exposure time: 1 second

    • Anti-CTNNA3 antibody [EPR18307] (ab184916)
      Anti-CTNNA3 antibody [EPR18307] (ab184916)

    Protocols

    • Western blot protocols
    • Immunoprecipitation protocols
    • Immunohistochemistry protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet download

      Download

    Certificate of Compliance

    To download a Certificate of Compliance, please enter your Lot number below:

    References (2)

    Publishing research using ab184916? Please let us know so that we can cite the reference in this datasheet.

    ab184916 has been referenced in 2 publications.

    • Pavel M  et al. a-Catenin levels determine direction of YAP/TAZ response to autophagy perturbation. Nat Commun 12:1703 (2021). PubMed: 33731717
    • Ortega A  et al. Intercalated disc in failing hearts from patients with dilated cardiomyopathy: Its role in the depressed left ventricular function. PLoS One 12:e0185062 (2017). PubMed: 28934278

    Customer reviews and Q&As

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