Recombinant Anti-CTNNA3 antibody [EPR18307] (ab184916)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR18307] to CTNNA3
- Suitable for: WB, IHC-P, IP
- Reacts with: Mouse, Rat, Dog, Human
Related conjugates and formulations
Overview
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Product name
Anti-CTNNA3 antibody [EPR18307]
See all CTNNA3 primary antibodies -
Description
Rabbit monoclonal [EPR18307] to CTNNA3 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, IPmore details -
Species reactivity
Reacts with: Mouse, Rat, Dog, Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HEK-293, C2C12, Rat1, C6, PC-12, NIH/3T3 and MDCK whole cell lysates; Human fetal brain, fetal heart and fetal kidney lysates; Mouse and rat brain and heart lysates. IHC/P: Human cardiac muscle and colon cancer tissues; Mouse and rat cardiac muscle tissues. IP: HEK293 whole cell lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR18307 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab184916 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/5000. Detects a band of approximately 100 kDa (predicted molecular weight: 100 kDa).
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IHC-P |
1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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IP |
1/100.
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Notes |
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WB
1/5000. Detects a band of approximately 100 kDa (predicted molecular weight: 100 kDa). |
IHC-P
1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
IP
1/100. |
Target
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Function
May be involved in formation of stretch-resistant cell-cell adhesion complexes. -
Tissue specificity
Predominantly expressed in heart and testis. Expressed at lower levels in brain, kidney, liver and skeletal muscle. -
Sequence similarities
Belongs to the vinculin/alpha-catenin family. -
Cellular localization
Cytoplasm > cytoskeleton. Localizes to intercalated disks of cardiomyocytes and in peritubular myoid cells of testis, and colocalizes with CTNNA1 and CTNNA2. - Information by UniProt
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Database links
- Entrez Gene: 29119 Human
- Entrez Gene: 216033 Mouse
- Entrez Gene: 361839 Rat
- Omim: 607667 Human
- SwissProt: Q9UI47 Human
- SwissProt: Q65CL1 Mouse
- Unigene: 660362 Human
- Unigene: 444692 Mouse
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Alternative names
- 4930429L08Rik antibody
- 4933408A16 antibody
- Alpha catenin like protein antibody
see all
Images
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All lanes : Anti-CTNNA3 antibody [EPR18307] (ab184916) at 1/50000 dilution
Lane 1 : HEK-293 (Human epithelial cells from embryonic kidney) whole cell lysate
Lane 2 : C2C12 (Mouse myoblast cell line) whole cell lysate
Lane 3 : Rat1 (Rat fibroblast cell line) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution
Predicted band size: 100 kDa
Observed band size: 100 kDa
Exposure time: 3 minutesBlocking/Dilution buffer: 5% NFDM/TBST.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CTNNA3 antibody [EPR18307] (ab184916)
Immunohistochemical analysis of paraffin-embedded Human colon cancer tissue labeling CTNNA3 with ab184916 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Cytoplasmic and membrane staining on tumor cells of Human colonic adenocarcinoma is observed. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CTNNA3 antibody [EPR18307] (ab184916)
Immunohistochemical analysis of paraffin-embedded Mouse cardiac muscle tissue labeling CTNNA3 with ab184916 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Intercalated disks staining on Mouse cardiac muscle is observed. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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All lanes : Anti-CTNNA3 antibody [EPR18307] (ab184916) at 1/5000 dilution
Lane 1 : Human fetal brain lysate
Lane 2 : Human fetal heart lysate
Lane 3 : Human fetal kidney lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 100 kDa
Observed band size: 100 kDa
Exposure time: 30 secondsBlocking/Dilution buffer: 5% NFDM/TBST.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CTNNA3 antibody [EPR18307] (ab184916)
Immunohistochemical analysis of paraffin-embedded Human cardiac muscle tissue labeling CTNNA3 with ab184916 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Intercalated disks staining of normal Human cardiac muscle is observed. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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All lanes : Anti-CTNNA3 antibody [EPR18307] (ab184916) at 1/5000 dilution
Lane 1 : Mouse brain lysate
Lane 2 : Mouse heart lysate
Lane 3 : Rat brain lysate
Lane 4 : Rat heart lysate
Lane 5 : C6 (Rat glial tumor cells) whole cell lysate
Lane 6 : PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysate
Lane 7 : NIH/3T3 (Mouse embyro fibroblast cells) whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution
Predicted band size: 100 kDa
Observed band size: 100 kDa
Exposure time: 1 secondBlocking/Dilution buffer: 5% NFDM/TBST.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CTNNA3 antibody [EPR18307] (ab184916)
Immunohistochemical analysis of paraffin-embedded Rat cardiac muscle tissue labeling CTNNA3 with ab184916 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Intercalated disks staining on Rat cardiac muscle is observed. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Anti-CTNNA3 antibody [EPR18307] (ab184916) at 1/50000 dilution + MDCK (Canine kidney cell line) whole cell lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution
Predicted band size: 100 kDa
Observed band size: 100 kDa
Exposure time: 1 secondBlocking/Dilution buffer: 5% NFDM/TBST.
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CTNNA3 was immunoprecipitated from 1mg of HEK-293 (Human epithelial cells from embryonic kidney) whole cell lysate with ab184916 at 1/100 dilution. Western blot was performed from the immunoprecipitate using ab184916 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.
Lane 1: HEK-293 whole cell lysate, 10 µg (Input).
Lane 2: ab184916 IP in HEK-293 whole cell lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab184916 in HEK-293 whole cell lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.Exposure time: 1 second
Protocols
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (2)
ab184916 has been referenced in 2 publications.
- Pavel M et al. a-Catenin levels determine direction of YAP/TAZ response to autophagy perturbation. Nat Commun 12:1703 (2021). PubMed: 33731717
- Ortega A et al. Intercalated disc in failing hearts from patients with dilated cardiomyopathy: Its role in the depressed left ventricular function. PLoS One 12:e0185062 (2017). PubMed: 28934278