Overview

  • Product name

    Anti-CTNNBIP1/ICAT antibody [EPR6697(2)]
    See all CTNNBIP1/ICAT primary antibodies
  • Description

    Rabbit monoclonal [EPR6697(2)] to CTNNBIP1/ICAT
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IP, Flow Cytmore details
    Unsuitable for: ICC or IHC-P
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide within Human CTNNBIP1/ICAT (N terminal). The exact sequence is proprietary.

  • Positive control

    • Human placenta, JAR, SW480, 293T and NCCIT cell lysates; permeabilized JAR cells.
  • General notes

     

     

     This product was previously labelled as CTNNBIP1

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab129011 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 11 kDa (predicted molecular weight: 9 kDa).
IP 1/10 - 1/100.
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

  • Application notes
    Is unsuitable for ICC or IHC-P.
  • Target

    Images

    • All lanes : Anti-CTNNBIP1/ICAT antibody [EPR6697(2)] (ab129011) at 1/1000 dilution

      Lane 1 : Human placenta cell lysate
      Lane 2 : JAR cell lysate
      Lane 3 : SW480 cell lysate
      Lane 4 : 293T cell lysate
      Lane 5 : NCCIT cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled Goat anti-Rabbit IgG at 1/2000 dilution

      Predicted band size: 9 kDa

    • ab129011, at 1/10 diltuion, staining CTNNBIP1/ICAT in permeabilized JAR cells by Flow Cytometry (red) or Rabbit IgG negative control (green).

    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References

    This product has been referenced in:

    • Fu X  et al. Linc00210 drives Wnt/ß-catenin signaling activation and liver tumor progression through CTNNBIP1-dependent manner. Mol Cancer 17:73 (2018). Read more (PubMed: 29540185) »
    • Li X  et al. SAMMSON drives the self-renewal of liver tumor initiating cells through EZH2-dependent Wnt/ß-catenin activation. Oncotarget 8:103785-103796 (2017). Read more (PubMed: 29262600) »
    See all 2 Publications for this product

    Customer reviews and Q&As

    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (liver)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    500 µg
    Specification
    liver
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: RT°C

    Abcam user community

    Verified customer

    Submitted Aug 10 2015

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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