Anti-CTNNBL1 antibody (ab95170)
Key features and details
- Rabbit polyclonal to CTNNBL1
- Suitable for: WB, IP, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-CTNNBL1 antibody
See all CTNNBL1 primary antibodies -
Description
Rabbit polyclonal to CTNNBL1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IP, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Rabbit, Horse, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Chinese hamster, Orangutan, Elephant
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Immunogen
Synthetic peptide corresponding to a region within N terminal amino acids 1-50 (MDVGELLSYQ PNRGTKRPRD DEEEEQKMRR KQTGTRERGR YREEEMTVVE) of Human CTNNBL1 (NP_110517.2).
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Positive control
- HeLa, 293T and NIH-3T3 whole cell lysates
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab95170 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/2000 - 1/10000. Predicted molecular weight: 65 kDa.
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| IP |
Use at 2-5 µg/mg of lysate.
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| IHC-P |
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| Notes |
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WB
1/2000 - 1/10000. Predicted molecular weight: 65 kDa. |
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IP
Use at 2-5 µg/mg of lysate. |
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IHC-P
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Induces apoptosis in CHO cells. -
Tissue specificity
Widely expressed with highest levels in skeletal muscle, placenta, heart, spleen, testis and thyroid. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 282421 Cow
- Entrez Gene: 56259 Human
- Entrez Gene: 66642 Mouse
- Entrez Gene: 296320 Rat
- Omim: 611537 Human
- SwissProt: O62703 Cow
- SwissProt: Q8WYA6 Human
- SwissProt: Q9CWL8 Mouse
see all -
Alternative names
- 5730471K09Rik antibody
- Beta catenin like 1 antibody
- Beta catenin like protein 1 antibody
see all
Images
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Western blot - Anti-CTNNBL1 antibody (ab95170)All lanes : Anti-CTNNBL1 antibody (ab95170) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Lane 5 : NIH3T3 whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 65 kDa
Exposure time: 3 minutes -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CTNNBL1 antibody (ab95170)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human testicular seminoma tissue labelling CTNNBL1 with ab95170 at 1/200 (1µg/ml). Detection: DAB. -
Immunoprecipitation - Anti-CTNNBL1 antibody (ab95170)Detection of CTNNBL1 in immunoprecipitates of HeLa whole cell lysate (1 mg for IP, 20% of IP loaded) using ab95170 at 3 µg/mg lysate for IP and at 0.4 µg/ml for subsequent Western blot detection.
Detection: Chemiluminescence with exposure time of 3 minutes.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab95170 has been referenced in 1 publication.
- Dong B et al. Detection of RACK1 and CTNNBL1-induced activation of mouse splenocytes using an immunoprecipitation-based technique. Mol Med Rep 16:7056-7063 (2017). PubMed: 28901482