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    ctnnbl1-antibody-ab95170.pdf

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Cell Biology Apoptosis Nucleus Other
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Anti-CTNNBL1 antibody (ab95170)

  • Datasheet
  • SDS
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Western blot - Anti-CTNNBL1 antibody (ab95170)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CTNNBL1 antibody (ab95170)
  • Immunoprecipitation - Anti-CTNNBL1 antibody (ab95170)

Key features and details

  • Rabbit polyclonal to CTNNBL1
  • Suitable for: WB, IP, IHC-P
  • Reacts with: Mouse, Human
  • Isotype: IgG

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Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

View more associated products

Overview

  • Product name

    Anti-CTNNBL1 antibody
    See all CTNNBL1 primary antibodies
  • Description

    Rabbit polyclonal to CTNNBL1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IP, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat, Rabbit, Horse, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Chinese hamster, Orangutan, Elephant
  • Immunogen

    Synthetic peptide corresponding to a region within N terminal amino acids 1-50 (MDVGELLSYQ PNRGTKRPRD DEEEEQKMRR KQTGTRERGR YREEEMTVVE) of Human CTNNBL1 (NP_110517.2).

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • HeLa, 293T and NIH-3T3 whole cell lysates
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 6.8
    Preservative: 0.09% Sodium azide
    Constituents: 0.1% BSA, Tris buffered saline
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Apoptosis
    • Nucleus
    • Other
    • Cell Biology
    • Other Antibodies
    • Other Antibodies
    • Cancer
    • Cell Death
    • Apoptosis
    • Nucleus
    • Other

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • HeLa whole cell lysate (ab29545)
    • NIH/3T3 whole cell lysate (ab7179)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab95170 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/2000 - 1/10000. Predicted molecular weight: 65 kDa.
IP
Use at 2-5 µg/mg of lysate.
IHC-P
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Notes
WB
1/2000 - 1/10000. Predicted molecular weight: 65 kDa.
IP
Use at 2-5 µg/mg of lysate.
IHC-P
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    Induces apoptosis in CHO cells.
  • Tissue specificity

    Widely expressed with highest levels in skeletal muscle, placenta, heart, spleen, testis and thyroid.
  • Cellular localization

    Nucleus.
  • Target information above from: UniProt accession Q8WYA6 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 282421 Cow
    • Entrez Gene: 56259 Human
    • Entrez Gene: 66642 Mouse
    • Entrez Gene: 296320 Rat
    • Omim: 611537 Human
    • SwissProt: O62703 Cow
    • SwissProt: Q8WYA6 Human
    • SwissProt: Q9CWL8 Mouse
    • SwissProt: Q4V8K2 Rat
    • Unigene: 472667 Human
    • Unigene: 45193 Mouse
    • Unigene: 98091 Rat
    see all
  • Alternative names

    • 5730471K09Rik antibody
    • Beta catenin like 1 antibody
    • Beta catenin like protein 1 antibody
    • Beta-catenin-like protein 1 antibody
    • C20orf33 antibody
    • Cadherin associated protein antibody
    • Catenin beta like 1 antibody
    • Chromosome 20 open reading frame 3 antibody
    • CTBL1_HUMAN antibody
    • CTNN protein antibody
    • CTNNBL 1 antibody
    • Ctnnbl1 antibody
    • dJ633O20.1 antibody
    • FLJ21108 antibody
    • NAP antibody
    • Nuclear associated protein antibody
    • nuclear protein NAP antibody
    • Nuclear-associated protein antibody
    • NYD SP19 antibody
    • OTTMUSP00000016933 antibody
    • P14 antibody
    • P14L antibody
    • PP8304 antibody
    • RP5 1118M15.1 antibody
    • Testis development protein NYD SP19 antibody
    • Testis development protein NYD-SP19 antibody
    see all

Images

  • Western blot - Anti-CTNNBL1 antibody (ab95170)
    Western blot - Anti-CTNNBL1 antibody (ab95170)
    All lanes : Anti-CTNNBL1 antibody (ab95170) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T whole cell lysate at 50 µg
    Lane 5 : NIH3T3 whole cell lysate at 50 µg

    Developed using the ECL technique.

    Predicted band size: 65 kDa


    Exposure time: 3 minutes
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CTNNBL1 antibody (ab95170)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CTNNBL1 antibody (ab95170)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human testicular seminoma tissue labelling CTNNBL1 with ab95170 at 1/200 (1µg/ml). Detection: DAB.
  • Immunoprecipitation - Anti-CTNNBL1 antibody (ab95170)
    Immunoprecipitation - Anti-CTNNBL1 antibody (ab95170)
    Detection of CTNNBL1 in immunoprecipitates of HeLa whole cell lysate (1 mg for IP, 20% of IP loaded) using ab95170 at 3 µg/mg lysate for IP and at 0.4 µg/ml for subsequent Western blot detection.
    Detection: Chemiluminescence with exposure time of 3 minutes.

Protocols

  • Western blot protocols
  • Immunoprecipitation protocols
  • Immunohistochemistry protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (1)

Publishing research using ab95170? Please let us know so that we can cite the reference in this datasheet.

ab95170 has been referenced in 1 publication.

  • Dong B  et al. Detection of RACK1 and CTNNBL1-induced activation of mouse splenocytes using an immunoprecipitation-based technique. Mol Med Rep 16:7056-7063 (2017). PubMed: 28901482

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