Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR8086(B)] to CTP synthase/CTPS (PE)
- Suitable for: Flow Cyt
- Reacts with: Human
- Conjugation: PE. Ex: 488nm, Em: 575nm
Product nameAnti-CTP synthase/CTPS antibody [EPR8086(B)] (PE)
See all CTP synthase/CTPS primary antibodies
DescriptionRabbit monoclonal [EPR8086(B)] to CTP synthase/CTPS (PE)
ConjugationPE. Ex: 488nm, Em: 575nm
Tested applicationsSuitable for: Flow Cytmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
corresponding to Human CTP synthase/CTPS aa 250-350.
- Flow Cyt: HeLa cells
This product was previously labelled as CTP synthase
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at +4°C. Avoid freeze / thaw cycle. Store In the Dark.
Dissociation constant (KD)KD = 1.28 x 10 -10 M Learn more about KD
Storage bufferpH: 7.40
Preservative: 0.01% Sodium azide
Constituents: 0.05% BSA, 49% PBS, 50% Glycerol
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab211349 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
The cellular localisation of this product has been verified in ICC/IF.
RelevanceCTP synthase (CTPS) catalyzes the ATP-dependent conversion of UTP to CTP using either L-glutamine or ammonia as the source of nitrogen. The enzyme is important in the biosynthesis nucleic acids. It has a key role in cell growth, development, and tumorigenesis.
Cellular localizationCytosol, membrane
- CTP synthase 1 antibody
- CTP synthase antibody
- CTP synthetase 1 antibody
Overlay histogram showing HeLa cells stained with ab211349 (red line). The cells were fixed with 4% formaldehyde (10 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab211349, 1/500 dilution) for 30 min at 22°C.
Isotype control antibody (black line) was Rabbit IgG (monoclonal) Phycoerythrin (ab209478) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.
Acquisition of >5,000 events were collected using a 50 mW Yellow/Green laser (561nm) and 586/15 bandpass filter.
This antibody gave a positive signal in HeLa cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Triton X-100 for 15 min used under the same conditions.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab211349 has not yet been referenced specifically in any publications.