Recombinant
RabMAb

Recombinant Anti-CTP synthase/CTPS antibody [EPR8086(B)] (Phycoerythrin) (ab211349)

Overview

  • Product name

    Anti-CTP synthase/CTPS antibody [EPR8086(B)] (Phycoerythrin)
    See all CTP synthase/CTPS primary antibodies
  • Description

    Rabbit monoclonal [EPR8086(B)] to CTP synthase/CTPS (Phycoerythrin)
  • Host species

    Rabbit
  • Conjugation

    Phycoerythrin. Ex: 488nm, Em: 575nm
  • Tested applications

    Suitable for: Flow Cytmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    corresponding to Human CTP synthase/CTPS aa 250-350.

  • Positive control

    • Flow Cyt: HeLa cells
  • General notes

     

     

     This product was previously labelled as CTP synthase

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab211349 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/500.

The cellular localisation of this product has been verified in ICC/IF.

Target

  • Relevance

    CTP synthase (CTPS) catalyzes the ATP-dependent conversion of UTP to CTP using either L-glutamine or ammonia as the source of nitrogen. The enzyme is important in the biosynthesis nucleic acids. It has a key role in cell growth, development, and tumorigenesis.
  • Cellular localization

    Cytosol, membrane
  • Database links

  • Alternative names

    • CTP synthase 1 antibody
    • CTP synthase antibody
    • CTP synthetase 1 antibody
    • CTP synthetase antibody
    • CTPS antibody
    • Cytidine 5 prime triphosphate synthetase antibody
    • Cytidine 5' triphosphate synthetase antibody
    • IMD24 antibody
    • UTP ammonia ligase 1 antibody
    see all

Images

  • Overlay histogram showing HeLa cells stained with ab211349 (red line). The cells were fixed with 4% formaldehyde (10 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab211349, 1/500 dilution) for 30 min at 22°C.

    Isotype control antibody (black line) was Rabbit IgG (monoclonal) Phycoerythrin (ab209478) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.

    Acquisition of >5,000 events were collected using a 50 mW Yellow/Green laser (561nm) and 586/15 bandpass filter.

    This antibody gave a positive signal in HeLa cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Triton X-100 for 15 min used under the same conditions.

References

ab211349 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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