Recombinant
RabMAb

Recombinant Anti-CTPS2 antibody [EPR16735] (ab196016)

Overview

  • Product name

    Anti-CTPS2 antibody [EPR16735]
    See all CTPS2 primary antibodies
  • Description

    Rabbit monoclonal [EPR16735] to CTPS2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IF, Flow Cytmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human CTPS2 aa 50-150. The exact sequence is proprietary.
    Database link: Q9NRF8

  • Positive control

    • WB: HeLa, HepG2, 293, Raw264.7 and PC12 cell lysates; IHC-P: Human transitional cell carcinoma of bladder, Human stomach tissues; ICC/IF: K562 cells; Flow cytometry: HeLa cells
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab196016 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000. Detects a band of approximately 66 kDa (predicted molecular weight: 66 kDa).
IHC-P 1/1500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF 1/600.
Flow Cyt 1/540.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

Target

Images

  • Anti-CTPS2 antibody [EPR16735] (ab196016) at 1/20000 dilution + HeLa (Human epithelial cells from cervix adenocarcinoma) cell lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 66 kDa
    Observed band size: 66 kDa



    5% NFDM/TBST: Blocking and diluting buffer.

  • All lanes : Anti-CTPS2 antibody [EPR16735] (ab196016) at 1/2000 dilution

    Lane 1 : HepG2 (Human liver hepatocellular carcinoma) cell lysate
    Lane 2 : 293 (Human epithelial cells from embryonic kidney) cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 66 kDa
    Observed band size: 66 kDa



    5% NFDM/TBST: Blocking and diluting buffer.

  • All lanes : Anti-CTPS2 antibody [EPR16735] (ab196016) at 1/2000 dilution

    Lane 1 : Raw264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) cell lysate
    Lane 2 : PC12 (Rat adrenal gland pheochromocytoma) cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 66 kDa
    Observed band size: 66 kDa



    5% NFDM/TBST: Blocking and diluting buffer.

  • Immunohistochemical analysis of paraffin-embedded Human transitional cell carcinoma of bladder tissue labeling CTPS2 using ab196016 at 1/1500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasmic and Nuclear staining is observed. Counter stained with Hematoxylin.

    Negative control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    The staining pattern observed is consistent with what has been described in the literature PMID: 24870241 and PMID: 16179339.

  • Immunohistochemical analysis of paraffin-embedded Human stomach tissue labeling CTPS2 using ab196016 at 1/1500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasmic and Nuclear staining is observed. Counter stained with Hematoxylin.

    Negative control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    The staining pattern observed is consistent with what has been described in the literature PMID: 24870241 and PMID: 16179339.

  • Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized K562 (Human chronic myelogenous leukemia cells from bone marrow) cells labeling CTPS2 with ab196016 at 1/600 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution (green). Cytoplasmic and Nuclear staining is observed. The nuclear counter stain is DAPI (blue).
    Negative control obtained using PBS instead of ab196016 followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution.

    The staining pattern observed is consistent with what has been described in the literature PMID: 16179339 and 24870241.

  • Flow cytometry analysis of HeLa (Human epithelial cells from cervix adenocarcinoma) cells labeling CTPS2 using ab196016 at 1/540 dilution (Red). A Goat anti rabbit IgG (FITC) at 1/150 dilution was used as secondary antibody. Cells were fixed with 2% paraformaldehyde. Cells without incubation with primary antibody and secondary antibody (Blue). Rabbit monoclonal IgG was used as isotype control (Black).

References

ab196016 has not yet been referenced specifically in any publications.

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