Overview

  • Product name

  • Description

    Rabbit polyclonal to CTRB1
  • Host species

    Rabbit
  • Specificity

    ab86177 recognizes both forms of this proteolytic enzyme.
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Pig
  • Immunogen

    Synthetic peptide from within a region of internal sequence amino acids 107-156 (KNPKFSILTV NNDITLLKLA TPARFSQTVS AVCLPSADDD FPAGTLCATT) of Human CTRB1 (NP_001897)

  • Positive control

    • Human fetal muscle lysate

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituents: PBS, 2% Sucrose
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab86177 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 28 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • Relevance

    CTRB1 (Chymotrypsinogen B1) is the inactive precursor of Alpha chymotrypsin, one of a family of serine proteases secreted into the gastrointestinal tract. The zymogen is activated by proteolytic cleavage by trypsin.
  • Cellular localization

    Secreted, extracellular space.
  • Database links

  • Alternative names

    • Chymotrypsinogen B antibody
    • Chymotrypsinogen B1 antibody
    • ChymotrypsinogenB antibody
    • ChymotrypsinogenB1 antibody
    • CTRB 1 antibody
    • CTRB antibody
    • FLJ42412 antibody
    • MGC88037 antibody
    • OTTHUMP00000214638 antibody
    see all

Images

  • Anti-CTRB1 antibody (ab86177) at 1 µg/ml (5% skim milk/ PBS buffer) + fetal muscle lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size: 28 kDa



    Gel concentration: 12%

References

ab86177 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Application
Western blot
Sample
Mouse Serum (serum (with pancreatic tumor))
Loading amount
30 µg
Specification
serum (with pancreatic tumor)
Gel Running Conditions
Reduced Denaturing (8%)
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 4% · Temperature: 25°C

Abcam user community

Verified customer

Submitted May 24 2012

Answer

Thank you for your inquiry.

Unfortunately, I am unable to issue discount code for the following antibodies:

ab60720, ab86704 and ab65109.

The immunogens for the MUC antibodies do not show a high enough homology (70% and below)to mouse to expect any cross-reactivity. Unfortunately, this is also true for the other antibodies we offer against these targets too.

ab86704 is already tested and guaranteed for mouse and WB. Please reassure your customer that the Abpromise guarantee will apply to this antibody in their experiment.

DISCOUNT CODEs:

ab68715: xxxxx


ab86177: xxxxx


ab21156: xxxxx

Expiration date:17. May 2012

I am very pleased to hear your customer would like to accept our offer and test ab68715 and ab86177 and ab21156 in mouse.

The codes will give your customer 1 freeprimary antibody eachbefore the expiration date.

To redeem this offer, please submit an Abreview eachformouse and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests.

The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways:

1) Call to place your order and mention the code to our customer service department;

2) Include the code in your fax order;

3) Place your order on the web and enter the promotional code.

Any feedback that your customer can provide will be greatly appreciated, whether positive or negative. If you or your customer has any further questions, please do not hesitate to contact us. We look forward to receiving your customer's Abreview and wish your customer luck with their research.

The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

Read More

Answer

Thank you for your enquiry and your interest. Although, ab86177 has not been tested on either chymotrypsin or chymotypsinogen - only in samples which contain the precursor and the cleaved forms - based on the peptide immunogen used to raise this antibody, it will recognize both forms of this proteolytic enzyme. We will update our on-line datasheet accordingly. If you need any further assistance in the future, please do not hesitate to contact me.

Read More

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up