Product nameAnti-CTRP4 antibody
DescriptionRabbit polyclonal to CTRP4
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Species reactivityReacts with: Mouse, Rat, Human
A 16 amino acid peptide from near the carboxy terminus of human CTRP4.
- Rat brain cell lysate.
Storage instructionsShipped at 4°C. Store at +4°C.
Storage bufferPreservative: 0.02% Sodium azide
Concentration information loading...
PurityImmunogen affinity purified
Immunizing Peptide (Blocking)
Our Abpromise guarantee covers the use of ab36871 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 - 4 µg/ml. Detects a band of approximately 35 kDa (predicted molecular weight: 37 kDa).Can be blocked with Human CTRP4 peptide (ab39741).|
|IHC-P||Use a concentration of 10 µg/ml.|
|ICC/IF||Use a concentration of 20 µg/ml.|
RelevanceAdipose tissue of an organism plays a major role in regulating physiologic and pathologic processes such as metabolism and immunity by producing and secreting a variety of bioactive molecules termed adipokines. One highly conserved family of adipokines is adiponectin/ACRP30 and its structural and functional paralogs, the C1q/tumor necrosis factor-alpha-related proteins (CTRPs) 1-7. Unlike adiponectin, which is expressed exclusively by differentiated adipocytes, the CTRPs are expressed in a wide variety of tissues. These proteins are thought to act mainly on liver and muscle tissue to control glucose and lipid metabolism. An analysis of the crystal structure of adiponectin revealed a structural and evolutionary link between TNF and C1q-containing proteins, suggesting that these proteins arose from a common ancestral innate immunity gene. Multiple isoforms of mouse CTRP4 have been reported.
- C1q and tumor necrosis factor related protein 4 antibody
- C1QTNF4 antibody
- Complement C1q tumor necrosis factor related protein antibody
- ZACRP4 antibody
Lane 1 : Anti-CTRP4 antibody (ab36871) at 1 µg/ml
Lane 2 : Anti-CTRP4 antibody (ab36871) at 2 µg/ml
Lane 3 : Anti-CTRP4 antibody (ab36871) at 4 µg/ml
All lanes : Rat brain cell lysate
Lysates/proteins at 15 µg/ml per lane.
All lanes : anti-rabbit IgG HRP at 1/10000 dilution
Predicted band size: 37 kDa
Observed band size: 35 kDa why is the actual band size different from the predicted?
Lanes read from left to right 1, 2 and 3. These proteins are often highly modified post-translationally and may migrate in SDS-PAGE at positions other than their predicted size.
ab36891 at 10µg/ml staining CTRP4 in rat brain by IHC
Immunofluorescence of CTRP4 in Rat Brain cells using ab36871 at 20 ug/ml.
This product has been referenced in:
- Li Q et al. Ctrp4, a new adipokine, promotes the differentiation of osteoblasts. Biochem Biophys Res Commun 512:224-229 (2019). Read more (PubMed: 30885436) »
- Byerly MS et al. C1q/TNF-related protein 4 (CTRP4) is a unique secreted protein with two tandem C1q domains that functions in the hypothalamus to modulate food intake and body weight. J Biol Chem 289:4055-69 (2014). WB ; Mouse . Read more (PubMed: 24366864) »