Key features and details
- Rabbit polyclonal to CTRP4
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Product nameAnti-CTRP4 antibody
DescriptionRabbit polyclonal to CTRP4
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Species reactivityReacts with: Mouse, Rat, Human
A 16 amino acid peptide from near the carboxy terminus of human CTRP4.
- Rat brain cell lysate.
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C.
Storage bufferpH: 7.2
Preservative: 0.02% Sodium azide
Concentration information loading...
PurityImmunogen affinity purified
Immunizing Peptide (Blocking)
Our Abpromise guarantee covers the use of ab36871 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 - 4 µg/ml. Detects a band of approximately 35 kDa (predicted molecular weight: 37 kDa).Can be blocked with Human CTRP4 peptide (ab39741).|
|IHC-P||Use a concentration of 10 µg/ml.|
|ICC/IF||Use a concentration of 20 µg/ml.|
RelevanceAdipose tissue of an organism plays a major role in regulating physiologic and pathologic processes such as metabolism and immunity by producing and secreting a variety of bioactive molecules termed adipokines. One highly conserved family of adipokines is adiponectin/ACRP30 and its structural and functional paralogs, the C1q/tumor necrosis factor-alpha-related proteins (CTRPs) 1-7. Unlike adiponectin, which is expressed exclusively by differentiated adipocytes, the CTRPs are expressed in a wide variety of tissues. These proteins are thought to act mainly on liver and muscle tissue to control glucose and lipid metabolism. An analysis of the crystal structure of adiponectin revealed a structural and evolutionary link between TNF and C1q-containing proteins, suggesting that these proteins arose from a common ancestral innate immunity gene. Multiple isoforms of mouse CTRP4 have been reported.
- C1q and tumor necrosis factor related protein 4 antibody
- C1QTNF4 antibody
- Complement C1q tumor necrosis factor related protein antibody
- ZACRP4 antibody
Lane 1 : Anti-CTRP4 antibody (ab36871) at 1 µg/ml
Lane 2 : Anti-CTRP4 antibody (ab36871) at 2 µg/ml
Lane 3 : Anti-CTRP4 antibody (ab36871) at 4 µg/ml
All lanes : Rat brain cell lysate
Lysates/proteins at 15 µg/ml per lane.
All lanes : anti-rabbit IgG HRP at 1/10000 dilution
Predicted band size: 37 kDa
Observed band size: 35 kDa why is the actual band size different from the predicted?
Lanes read from left to right 1, 2 and 3. These proteins are often highly modified post-translationally and may migrate in SDS-PAGE at positions other than their predicted size.
ab36891 at 10µg/ml staining CTRP4 in rat brain by IHC
Immunofluorescence of CTRP4 in Rat Brain cells using ab36871 at 20 ug/ml.
ab36871 has been referenced in 3 publications.
- Li Q et al. Ctrp4, a new adipokine, promotes the differentiation of osteoblasts. Biochem Biophys Res Commun 512:224-229 (2019). PubMed: 30885436
- Bando H et al. CXCR4 regulates Plasmodium development in mouse and human hepatocytes. J Exp Med 216:1733-1748 (2019). PubMed: 31189656
- Byerly MS et al. C1q/TNF-related protein 4 (CTRP4) is a unique secreted protein with two tandem C1q domains that functions in the hypothalamus to modulate food intake and body weight. J Biol Chem 289:4055-69 (2014). WB ; Mouse . PubMed: 24366864