Product nameAnti-CTRP5 antibody
See all CTRP5 primary antibodies
DescriptionRabbit polyclonal to CTRP5
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Mouse, Rat, Human
A 15 amino acid peptide from near the amino terminus of human CTRP5.
- Human brain cell lysate.
Storage instructionsShipped at 4°C. Store at +4°C.
Storage bufferPreservative: 0.02% Sodium azide
Concentration information loading...
PurityImmunogen affinity purified
Immunizing Peptide (Blocking)
Our Abpromise guarantee covers the use of ab36893 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 - 4 µg/ml. Detects a band of approximately 39 kDa (predicted molecular weight: 25 kDa).Can be blocked with Human CTRP5 peptide (ab39744).|
|IHC-P||Use a concentration of 10 µg/ml.|
RelevanceAdipose tissue of an organism plays a major role in regulating physiologic and pathologic processes such as metabolism and immunity by producing and secreting a variety of bioactive molecules termed adipokines. One highly conserved family of adipokines is adiponectin/ACRP30 and its structural and functional paralogs, the C1q/tumor necrosis factor-alpha-related proteins (CTRPs) 1-7. Unlike adiponectin, which is expressed exclusively by differentiated adipocytes, the CTRPs are expressed in a wide variety of tissues. These proteins are thought to act mainly on liver and muscle tissue to control glucose and lipid metabolism. An analysis of the crystal structure of adiponectin revealed a structural and evolutionary link between TNF and C1q-containing proteins, suggesting that these proteins arose from a common ancestral innate immunity gene. CTRP5 has been suggested to be involved in age-related macular degeneration.
- C1q and tumor necrosis factor related protein 5 antibody
- C1QTNF5 antibody
- Complement C1q tumor necrosis factor related protein 5 precursor antibody
- LORD antibody
ab36893 staining CTRP5 in human pancreas carcinoma tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde, permeabilized with 0.2% Triton X-100 in PBS and blocked with 5% milk for 30 minutes at room temperature; antigen retrieval was by heat mediation in 10mM sodium citrate + 0.05% Tween. Samples were incubated with primary antibody (1/750 in PBS) for 16 hours at 4°C. An undiluted Biotin-conjugated goat anti-rabbit IgG polyclonal was used as the secondary antibody.
Lane 1 : Anti-CTRP5 antibody (ab36893) at 1 µg/ml
Lane 2 : Anti-CTRP5 antibody (ab36893) at 2 µg/ml
Lane 3 : Anti-CTRP5 antibody (ab36893) at 4 µg/ml
All lanes : Human brain cell lysate
Lysates/proteins at 15 µg/ml per lane.
All lanes : Anti-rabbit IgG HRP at 1/10000 dilution
Predicted band size: 25 kDa
Observed band size: 39 kDa why is the actual band size different from the predicted?
Lanes read from left to right, 1-3. These proteins are often highly modified post-translationally and may migrate in SDS-PAGE at positions other than their predicted size.
ab36993 at 10µg/ml staining CTRP5 in human brain tissue by IHC
ab36893 has not yet been referenced specifically in any publications.