Anti-CTRP5 antibody (ab36893)
Key features and details
- Rabbit polyclonal to CTRP5
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-CTRP5 antibody
See all CTRP5 primary antibodies -
Description
Rabbit polyclonal to CTRP5 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Human
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Immunogen
A 15 amino acid peptide from near the amino terminus of human CTRP5.
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Positive control
- Human brain cell lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab36893 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| Application | Species |
|---|---|
| IHC-P |
Human
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| WB |
Human
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| Application | Abreviews | Notes |
|---|---|---|
| WB |
Use a concentration of 1 - 4 µg/ml. Detects a band of approximately 39 kDa (predicted molecular weight: 25 kDa).
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| IHC-P | (3) |
Use a concentration of 10 µg/ml.
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| Notes |
|---|
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WB
Use a concentration of 1 - 4 µg/ml. Detects a band of approximately 39 kDa (predicted molecular weight: 25 kDa). |
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IHC-P
Use a concentration of 10 µg/ml. |
Target
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Relevance
Adipose tissue of an organism plays a major role in regulating physiologic and pathologic processes such as metabolism and immunity by producing and secreting a variety of bioactive molecules termed adipokines. One highly conserved family of adipokines is adiponectin/ACRP30 and its structural and functional paralogs, the C1q/tumor necrosis factor-alpha-related proteins (CTRPs) 1-7. Unlike adiponectin, which is expressed exclusively by differentiated adipocytes, the CTRPs are expressed in a wide variety of tissues. These proteins are thought to act mainly on liver and muscle tissue to control glucose and lipid metabolism. An analysis of the crystal structure of adiponectin revealed a structural and evolutionary link between TNF and C1q-containing proteins, suggesting that these proteins arose from a common ancestral innate immunity gene. CTRP5 has been suggested to be involved in age-related macular degeneration. -
Cellular localization
Secreted -
Database links
- Entrez Gene: 114902 Human
- Omim: 605670 Human
- SwissProt: Q9BXJ0 Human
- Unigene: 632102 Human
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Alternative names
- C1q and tumor necrosis factor related protein 5 antibody
- C1QTNF5 antibody
- Complement C1q tumor necrosis factor related protein 5 precursor antibody
- LORD antibody
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CTRP5 antibody (ab36893)This image is courtesy of an Abreview submitted by Steffen Rickelt
ab36893 staining CTRP5 in human pancreas carcinoma tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde, permeabilized with 0.2% Triton X-100 in PBS and blocked with 5% milk for 30 minutes at room temperature; antigen retrieval was by heat mediation in 10mM sodium citrate + 0.05% Tween. Samples were incubated with primary antibody (1/750 in PBS) for 16 hours at 4°C. An undiluted Biotin-conjugated goat anti-rabbit IgG polyclonal was used as the secondary antibody.
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Western blot - Anti-CTRP5 antibody (ab36893)Lane 1 : Anti-CTRP5 antibody (ab36893) at 1 µg/ml
Lane 2 : Anti-CTRP5 antibody (ab36893) at 2 µg/ml
Lane 3 : Anti-CTRP5 antibody (ab36893) at 4 µg/ml
All lanes : Human brain cell lysate
Lysates/proteins at 15 µg/ml per lane.
Secondary
All lanes : Anti-rabbit IgG HRP at 1/10000 dilution
Predicted band size: 25 kDa
Observed band size: 39 kDa why is the actual band size different from the predicted?
Lanes read from left to right, 1-3. These proteins are often highly modified post-translationally and may migrate in SDS-PAGE at positions other than their predicted size. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CTRP5 antibody (ab36893)ab36993 at 10µg/ml staining CTRP5 in human brain tissue by IHC
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab36893 has not yet been referenced specifically in any publications.