Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: RT°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris pH 9.0
Sample
Human Tissue sections (human colon muscle)
Specification
human colon muscle
Permeabilization
Yes - 0.2% Triton X-100 and PBS
Fixative
Formaldehyde
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: overnight in PBS
Dilution
1/1000
Secondary antibody
Name
Non-Abcam antibody was used: Vectastain ABC Kit
Host species: Goat
Clonality: Polyclonal
Conjugation: Biotin
Host species: Goat
Clonality: Polyclonal
Conjugation: Biotin
Additional data
Additional Notes
IHC Vector Laboratories DAB Substrate for peroxidase + nickel was used.
Mrs. Maria Cecilia Ybanez
Verified customer
Submitted Apr 07 2014