Overview

  • Product name

    Anti-CTU2/NCS2 antibody [EPR11194]
    See all CTU2/NCS2 primary antibodies
  • Description

    Rabbit monoclonal [EPR11194] to CTU2/NCS2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, Flow Cytmore details
    Unsuitable for: ICC or IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human CTU2/NCS2 aa 300-400 (Cysteine residue). The exact sequence is proprietary.
    Database link: Q2VPK5

  • Positive control

    • HT29, K562, HeLa and Human testis lysates. Human colonic carcinoma and kidney tissues. Permeabilized HT29 cells.
  • General notes

     This product was previously labelled as CTU2

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR11194
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab177160 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Detects a band of approximately 56 kDa (predicted molecular weight: 56 kDa).
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

  • Application notes
    Is unsuitable for ICC or IP.
  • Target

    • Function

      Plays a central role in 2-thiolation of mcm(5)S(2)U at tRNA wobble positions of tRNA(Lys), tRNA(Glu) and tRNA(Gln). May act by forming a heterodimer with CTU1/ATPBD3 that ligates sulfur from thiocarboxylated URM1 onto the uridine of tRNAs at wobble position.
    • Pathway

      tRNA modification; 5-methoxycarbonylmethyl-2-thiouridine-tRNA biosynthesis.
    • Sequence similarities

      Belongs to the CTU2/NCS2 family.
    • Cellular localization

      Cytoplasm.
    • Information by UniProt
    • Database links

    • Alternative names

      • C16orf84 antibody
      • ctu2 antibody
      • CTU2_HUMAN antibody
      • Cytoplasmic tRNA 2-thiolation protein 2 antibody
      • cytosolic thiouridylase subunit 2 homolog (S. pombe) antibody
      • NCS2 antibody
      • PF0432 antibody
      see all

    Images

    • All lanes : Anti-CTU2/NCS2 antibody [EPR11194] (ab177160) at 1/1000 dilution

      Lane 1 : HT29 lysate
      Lane 2 : K562 lysate
      Lane 3 : HeLa lysate
      Lane 4 : Human testis lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size: 56 kDa

    • Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling CTU2/NCS2 with ab177160 at 1/50 dilution.

    • Flow cytometric analysis of permeabilized HT29 cells labeling CTU2/NCS2 with ab177160 at 1/10 dilution (red), or a rabbit IgG (negative) (green).

    • Immunohistochemical analysis of paraffin-embedded Human colonic carcinoma tissue labeling CTU2/NCS2  with ab177160 at 1/50 dilution.

    References

    This product has been referenced in:

    • Delaunay S  et al. Elp3 links tRNA modification to IRES-dependent translation of LEF1 to sustain metastasis in breast cancer. J Exp Med 213:2503-2523 (2016). Read more (PubMed: 27811057) »
    See 1 Publication for this product

    Customer reviews and Q&As

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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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