Recombinant
RabMAb

Recombinant Anti-Cubilin antibody [EPR16259-27] - C-terminal (ab191073)

Overview

  • Product name

    Anti-Cubilin antibody [EPR16259-27] - C-terminal
    See all Cubilin primary antibodies
  • Description

    Rabbit monoclonal [EPR16259-27] to Cubilin - C-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human Cubilin aa 3350-3550 (C terminal). The exact sequence is proprietary.
    Database link: O60494

  • Positive control

    • Human fetal kidney tissue lysate; Human kidney tissue (normal)
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab191073 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/500.
WB 1/10000 - 1/50000. Detects a band of approximately 399 kDa (predicted molecular weight: 399 kDa).

Target

  • Function

    Cotransporter which plays a role in lipoprotein, vitamin and iron metabolism, by facilitating their uptake. Binds to ALB, MB, Kappa and lambda-light chains, TF, hemoglobin, GC, SCGB1A1, APOA1, high density lipoprotein, and the GIF-cobalamin complex. The binding of all ligands requires calcium. Serves as important transporter in several absorptive epithelia, including intestine, renal proximal tubules and embryonic yolk sac. Interaction with LRP2 mediates its trafficking throughout vesicles and facilitates the uptake of specific ligands like GC, hemoglobin, ALB, TF and SCGB1A1. Interaction with AMN controls its trafficking to the plasma membrane and facilitates endocytosis of ligands. May play an important role in the development of the peri-implantation embryo through internalization of APOA1 and cholesterol. Binds to LGALS3 at the maternal-fetal interface.
  • Tissue specificity

    Expressed in kidney proximal tubule cells, placenta, visceral yolk-sac cells and in absorptive intestinal cells. Expressed in the epithelium of intestine and kidney.
  • Involvement in disease

    Recessive hereditary megaloblastic anemia 1
  • Sequence similarities

    Contains 27 CUB domains.
    Contains 7 EGF-like domains.
  • Domain

    The CUB domains 5 to 8 mediate binding to GIF and ALB. CUB domains 1 and 2 mediate interaction with LRP2.
  • Post-translational
    modifications

    The precursor is cleaved by a trans-Golgi proteinase furin. The result is a propeptide cleaved off.
    N-glycosylated.
  • Cellular localization

    Endosome membrane. Lysosome membrane. Colocalizes with AMN and LRP2 in the endocytotic apparatus of epithelial cells.
  • Information by UniProt
  • Database links

  • Alternative names

    • 460 kDa receptor antibody
    • cubilin (intrinsic factor-cobalamin receptor) antibody
    • Cubilin antibody
    • Cubilin precursor antibody
    • Cubn antibody
    • CUBN_HUMAN antibody
    • IFCR antibody
    • Intestinal intrinsic factor receptor antibody
    • intrinsic factor B12-receptor antibody
    • Intrinsic factor-cobalamin receptor antibody
    • Intrinsic factor-vitamin B12 receptor antibody
    • megaloblastic anemia 1 antibody
    • MGA1 antibody
    see all

Images

  • Anti-Cubilin antibody [EPR16259-27] - C-terminal (ab191073) at 1/50000 dilution + Human fetal kidney tissue lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 399 kDa
    Observed band size: 399 kDa

  • Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling Cubilin with ab191073 at a 1/500 dilution.

    Secondary antibody Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at a 1/1000 dilution. Counter stain Hematoxylin. Negative control also shown.

References

This product has been referenced in:

  • Yin W  et al. Protein kinase C and protein kinase A are involved in the protection of recombinant human glucagon-like peptide-1 on glomeruli and tubules in diabetic rats. J Diabetes Investig 10:613-625 (2019). Read more (PubMed: 30307132) »
See 1 Publication for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Sheep Tissue sections (Kidney)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: EDTA pH9.0
Permeabilization
Yes - 0.3%Tween in PBS
Specification
Kidney
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 20°C
Fixative
Formaldehyde

Dr. Timo Rademakers

Verified customer

Submitted Oct 01 2018

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Kidney)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate
Permeabilization
No
Specification
Kidney
Blocking step
Ventana blocking agent as blocking agent for 4 minute(s) · Concentration: 100% · Temperature: 36°C
Fixative
Formaldehyde

Abcam user community

Verified customer

Submitted May 12 2015

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