Anti-CUG-BP1 antibody [3B1] (ab9549)

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Immunocytochemistry/ Immunofluorescence - Anti-CUG-BP1 antibody [3B1] (ab9549)
  • Western blot - Anti-CUG-BP1 antibody [3B1] (ab9549)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CUG-BP1 antibody [3B1] (ab9549)

Key features and details

  • Mouse monoclonal [3B1] to CUG-BP1
  • Suitable for: IHC-P, ICC/IF, WB
  • Reacts with: Human
  • Isotype: IgG1

Overview

  • Product name

    Anti-CUG-BP1 antibody [3B1]
    See all CUG-BP1 primary antibodies

  • Description

    Mouse monoclonal [3B1] to CUG-BP1

  • Host species

    Mouse

  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    corresponding to CUG-BP1.

  • Positive control

    • WB: HeLa, HEK-293T and SHSY-5Y cell lysates; Human kidney and brain tissue lysates. IF/ICC: MCF7 cells. IHC: Human Breast cancer tissue

  • General notes

    This antibody clone is manufactured by Abcam.

    If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab9549 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
ICC/IF
Human
IHC-P
Human
WB
Human
All applications
Mouse
Rat
Rabbit
Cow
Pig
Application Abreviews Notes
IHC-P
Use a concentration of 5 µg/ml.
ICC/IF
Use a concentration of 10 µg/ml.
WB (1)
Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 54 kDa (predicted molecular weight: 54 kDa).
Notes
IHC-P
Use a concentration of 5 µg/ml.
ICC/IF
Use a concentration of 10 µg/ml.
WB
Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 54 kDa (predicted molecular weight: 54 kDa).

Target

  • Function

    RNA-binding protein implicated in the regulation of several post-transcriptional events. Involved in pre-mRNA alternative splicing, mRNA translation and stability. Mediates exon inclusion and/or exclusion in pre-mRNA that are subject to tissue-specific and developmentally regulated alternative splicing. Specifically activates exon 5 inclusion of cardiac isoforms of TNNT2 during heart remodeling at the juvenile to adult transition. Acts as both an activator and repressor of a pair of coregulated exons: promotes inclusion of the smooth muscle (SM) exon but exclusion of the non-muscle (NM) exon in actinin pre-mRNAs. Activates SM exon 5 inclusion by antagonizing the repressive effect of PTB. Promotes exclusion of exon 11 of the INSR pre-mRNA. Inhibits, together with HNRNPH1, insulin receptor (IR) pre-mRNA exon 11 inclusion in myoblast. Increases translation and controls the choice of translation initiation codon of CEBPB mRNA. Increases mRNA translation of CEBPB in aging liver (By similarity). Increases translation of CDKN1A mRNA by antagonizing the repressive effect of CALR3. Mediates rapid cytoplasmic mRNA deadenylation. Recruits the deadenylase PARN to the poly(A) tail of EDEN-containing mRNAs to promote their deadenylation. Required for completion of spermatogenesis (By similarity). Binds to (CUG)n triplet repeats in the 3'-UTR of transcripts such as DMPK and to Bruno response elements (BREs). Binds to muscle-specific splicing enhancer (MSE) intronic sites flanking the alternative exon 5 of TNNT2 pre-mRNA. Binds to AU-rich sequences (AREs or EDEN-like) localized in the 3'-UTR of JUN and FOS mRNAs. Binds to the IR RNA. Binds to the 5'-region of CDKN1A and CEBPB mRNAs. Binds with the 5'-region of CEBPB mRNA in aging liver.

  • Tissue specificity

    Ubiquitous.

  • Sequence similarities

    Belongs to the CELF/BRUNOL family.
    Contains 3 RRM (RNA recognition motif) domains.

  • Post-translational
    modifications

    Phosphorylated. Its phosphorylation status increases in senescent cells.

  • Cellular localization

    Nucleus. Cytoplasm. RNA-binding activity is detected in both nuclear and cytoplasmic compartments.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • 50 kDa Nuclear polyadenylated RNA binding protein antibody
    • 50 kDa nuclear polyadenylated RNA-binding protein antibody
    • Bruno like 2 antibody
    • bruno like protein 2 antibody
    • Bruno-like protein 2 antibody
    • BRUNOL 2 antibody
    • BRUNOL2 antibody
    • CELF 1 antibody
    • CELF-1 antibody
    • celf1 antibody
    • CELF1 CUGBP, Elav like family member 1 antibody
    • CELF1_HUMAN antibody
    • CUG BP and ETR 3 like factor 1 antibody
    • CUG BP antibody
    • CUG BP1 antibody
    • CUG RNA binding protein antibody
    • CUG triplet repeat RNA binding protein 1 antibody
    • CUG triplet repeat RNA-binding protein 1 antibody
    • CUG-BP antibody
    • CUG-BP- and ETR-3-like factor 1 antibody
    • CUG-BP1 antibody
    • CUGBP 1 antibody
    • CUGBP and ETR3 like factor 1 antibody
    • CUGBP antibody
    • CUGBP Elav like family member 1 antibody
    • CUGBP Elav-like family member 1 antibody
    • CUGBP1 antibody
    • Cytidine uridine guanosine binding protein 1 antibody
    • Deadenylation factor CUG BP antibody
    • Deadenylation factor CUG-BP antibody
    • Deadenylation factor CUGBP antibody
    • EDEN BP antibody
    • EDEN BP homolog antibody
    • EDEN-BP antibody
    • EDEN-BP homolog antibody
    • embryo deadenylation element binding protein antibody
    • embryo deadenylation element binding protein homolog antibody
    • Embryo deadenylation element-binding protein homolog antibody
    • hNab 50 antibody
    • hNab50 antibody
    • NAB 50 antibody
    • NAB50 antibody
    • NAPOR antibody
    • Nuclear polyadenylated RNA binding protein 50 kD antibody
    • Nuclear polyadenylated RNA binding protein antibody
    • RNA binding protein BRUNOL 2 antibody
    • RNA binding protein BRUNOL2 antibody
    • RNA-binding protein BRUNOL-2 antibody
    see all

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-CUG-BP1 antibody [3B1] (ab9549)
    Immunocytochemistry/ Immunofluorescence - Anti-CUG-BP1 antibody [3B1] (ab9549)

    ab9549 stained MCF7 cells. The cells were 100% methanol fixed for 5 minutes at -20°C and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1hour at room temperature to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab9549 at 10µg/ml) overnight at +4°C. The secondary antibody (pseudo-colored green) was Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed (ab150117) used at a 1/1000 dilution for 1hour at room temperature. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1hour at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.

  • Western blot - Anti-CUG-BP1 antibody [3B1] (ab9549)
    Western blot - Anti-CUG-BP1 antibody [3B1] (ab9549)
    All lanes : Anti-CUG-BP1 antibody [3B1] (ab9549) at 1 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : SHSY-5Y (Human neuroblastoma cell line) Whole Cell Lysate
    Lane 3 : Human kidney tissue lysate - total protein (ab30203)

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 54 kDa
    Observed band size: 54 kDa


    Exposure time: 20 minutes
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CUG-BP1 antibody [3B1] (ab9549)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CUG-BP1 antibody [3B1] (ab9549)

    IHC image of CUG-BP1 staining in human normal kidney formalin fixed paraffin embedded tissue section*, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab9549, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

     

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

References (16)

Publishing research using ab9549? Please let us know so that we can cite the reference in this datasheet.

ab9549 has been referenced in 16 publications.

  • Idris M  et al. The MBNL/CELF Splicing Factors Regulate Cytosolic Sulfotransferase 4A1 Protein Expression during Cell Differentiation. Drug Metab Dispos 47:314-319 (2019). PubMed: 30606728
  • Rong Z  et al. Quantitative Studies of Muscleblind Proteins and Their Interaction With TCF4 RNA Foci Support Involvement in the Mechanism of Fuchs' Dystrophy. Invest Ophthalmol Vis Sci 60:3980-3991 (2019). PubMed: 31560764
  • Siddam AD  et al. The RNA-binding protein Celf1 post-transcriptionally regulates p27Kip1 and Dnase2b to control fiber cell nuclear degradation in lens development. PLoS Genet 14:e1007278 (2018). PubMed: 29565969
  • Nutter CA  et al. Developmentally regulated alternative splicing is perturbed in type 1 diabetic skeletal muscle. Muscle Nerve 56:744-749 (2017). PubMed: 28164326
  • Bai Z  et al. Dynamic transcriptome changes during adipose tissue energy expenditure reveal critical roles for long noncoding RNA regulators. PLoS Biol 15:e2002176 (2017). WB . PubMed: 28763438
View all Publications for this product

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Western blot abreview for Anti-CUG-BP1 antibody [3B1]

 Excellent
Abreviews
abreview image
Application
Western blot
Sample
Mouse Tissue lysate - whole (fetal lung)
Loading amount
20 µg
Specification
fetal lung
Gel Running Conditions
Reduced Denaturing (12%)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
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Submitted Feb 20 2013

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