Recombinant
Recombinant Anti-CUG-BP1 antibody [EPR8298(B)] (ab129115)
- Datasheet
- References (4)
- Protocols
Overview
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Product name
Anti-CUG-BP1 antibody [EPR8298(B)]
See all CUG-BP1 primary antibodies -
Description
Rabbit monoclonal [EPR8298(B)] to CUG-BP1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IP, IHC-P, Flow Cyt, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide within Human CUG-BP1 aa 1-100 (N terminal). The exact sequence is proprietary.
Database link: Q92879 -
Positive control
- HeLa and SH-SY5Y cell lysates.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Dissociation constant (KD)
KD = 3.76 x 10 -10 M Learn more about KD -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR8298(B) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab129115 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/10000. Detects a band of approximately 52 kDa (predicted molecular weight: 52 kDa). | |
| IP | 1/10 - 1/100. | |
| IHC-P | 1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. | |
| Flow Cyt | 1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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| ICC/IF | 1/50 - 1/100. |
Target
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Function
RNA-binding protein implicated in the regulation of several post-transcriptional events. Involved in pre-mRNA alternative splicing, mRNA translation and stability. Mediates exon inclusion and/or exclusion in pre-mRNA that are subject to tissue-specific and developmentally regulated alternative splicing. Specifically activates exon 5 inclusion of cardiac isoforms of TNNT2 during heart remodeling at the juvenile to adult transition. Acts as both an activator and repressor of a pair of coregulated exons: promotes inclusion of the smooth muscle (SM) exon but exclusion of the non-muscle (NM) exon in actinin pre-mRNAs. Activates SM exon 5 inclusion by antagonizing the repressive effect of PTB. Promotes exclusion of exon 11 of the INSR pre-mRNA. Inhibits, together with HNRNPH1, insulin receptor (IR) pre-mRNA exon 11 inclusion in myoblast. Increases translation and controls the choice of translation initiation codon of CEBPB mRNA. Increases mRNA translation of CEBPB in aging liver (By similarity). Increases translation of CDKN1A mRNA by antagonizing the repressive effect of CALR3. Mediates rapid cytoplasmic mRNA deadenylation. Recruits the deadenylase PARN to the poly(A) tail of EDEN-containing mRNAs to promote their deadenylation. Required for completion of spermatogenesis (By similarity). Binds to (CUG)n triplet repeats in the 3'-UTR of transcripts such as DMPK and to Bruno response elements (BREs). Binds to muscle-specific splicing enhancer (MSE) intronic sites flanking the alternative exon 5 of TNNT2 pre-mRNA. Binds to AU-rich sequences (AREs or EDEN-like) localized in the 3'-UTR of JUN and FOS mRNAs. Binds to the IR RNA. Binds to the 5'-region of CDKN1A and CEBPB mRNAs. Binds with the 5'-region of CEBPB mRNA in aging liver. -
Tissue specificity
Ubiquitous. -
Sequence similarities
Belongs to the CELF/BRUNOL family.
Contains 3 RRM (RNA recognition motif) domains. -
Post-translational
modificationsPhosphorylated. Its phosphorylation status increases in senescent cells. -
Cellular localization
Nucleus. Cytoplasm. RNA-binding activity is detected in both nuclear and cytoplasmic compartments. - Information by UniProt
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Database links
- Entrez Gene: 10658 Human
- Entrez Gene: 13046 Mouse
- Entrez Gene: 362160 Rat
- Omim: 601074 Human
- SwissProt: Q92879 Human
- SwissProt: P28659 Mouse
- SwissProt: Q4QQT3 Rat
- Unigene: 595333 Human
see all -
Alternative names
- 50 kDa Nuclear polyadenylated RNA binding protein antibody
- 50 kDa nuclear polyadenylated RNA-binding protein antibody
- Bruno like 2 antibody
see all
Images
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![Western blot - Anti-CUG-BP1 antibody [EPR8298(B)] (ab129115)](https://www.abcam.com/ps/products/129/ab129115/Images/ab129115-143126-anti-cug-bp1-antibody-epr8298b-western-blot.jpg)
Western blot - Anti-CUG-BP1 antibody [EPR8298(B)] (ab129115)All lanes : Anti-CUG-BP1 antibody [EPR8298(B)] (ab129115) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : SH-SY5Y cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Standard HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 52 kDa
Observed band size: 52 kDa -
![Other - Anti-CUG-BP1 antibody [EPR8298(B)] (ab129115)](https://www.abcam.com/ps/products/129/ab129115/Images/ab129115-143125-anti-cug-bp1-antibody-epr8298b.jpg)
Other - Anti-CUG-BP1 antibody [EPR8298(B)] (ab129115)
Protocols
Datasheets and documents
References
This product has been referenced in:
- Saul MJ et al. miR-574-5p as RNA decoy for CUGBP1 stimulates human lung tumor growth by mPGES-1 induction. FASEB J 33:6933-6947 (2019). Read more (PubMed: 30922080) »
- Yuan T et al. Low-density lipoprotein receptor-related protein 6 regulates alternative pre-mRNA splicing. J Cell Mol Med 22:4653-4663 (2018). Read more (PubMed: 30070011) »
