Overview

  • Product name

    Anti-CUG-BP1 antibody [EPR8298(B)]
    See all CUG-BP1 primary antibodies
  • Description

    Rabbit monoclonal [EPR8298(B)] to CUG-BP1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IP, IHC-P, Flow Cyt, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human CUG-BP1 aa 1-100 (N terminal). The exact sequence is proprietary.
    Database link: Q92879

  • Positive control

    • HeLa and SH-SY5Y cell lysates.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab129115 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 52 kDa (predicted molecular weight: 52 kDa).
IP 1/10 - 1/100.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

ICC/IF 1/50 - 1/100.

Target

  • Function

    RNA-binding protein implicated in the regulation of several post-transcriptional events. Involved in pre-mRNA alternative splicing, mRNA translation and stability. Mediates exon inclusion and/or exclusion in pre-mRNA that are subject to tissue-specific and developmentally regulated alternative splicing. Specifically activates exon 5 inclusion of cardiac isoforms of TNNT2 during heart remodeling at the juvenile to adult transition. Acts as both an activator and repressor of a pair of coregulated exons: promotes inclusion of the smooth muscle (SM) exon but exclusion of the non-muscle (NM) exon in actinin pre-mRNAs. Activates SM exon 5 inclusion by antagonizing the repressive effect of PTB. Promotes exclusion of exon 11 of the INSR pre-mRNA. Inhibits, together with HNRNPH1, insulin receptor (IR) pre-mRNA exon 11 inclusion in myoblast. Increases translation and controls the choice of translation initiation codon of CEBPB mRNA. Increases mRNA translation of CEBPB in aging liver (By similarity). Increases translation of CDKN1A mRNA by antagonizing the repressive effect of CALR3. Mediates rapid cytoplasmic mRNA deadenylation. Recruits the deadenylase PARN to the poly(A) tail of EDEN-containing mRNAs to promote their deadenylation. Required for completion of spermatogenesis (By similarity). Binds to (CUG)n triplet repeats in the 3'-UTR of transcripts such as DMPK and to Bruno response elements (BREs). Binds to muscle-specific splicing enhancer (MSE) intronic sites flanking the alternative exon 5 of TNNT2 pre-mRNA. Binds to AU-rich sequences (AREs or EDEN-like) localized in the 3'-UTR of JUN and FOS mRNAs. Binds to the IR RNA. Binds to the 5'-region of CDKN1A and CEBPB mRNAs. Binds with the 5'-region of CEBPB mRNA in aging liver.
  • Tissue specificity

    Ubiquitous.
  • Sequence similarities

    Belongs to the CELF/BRUNOL family.
    Contains 3 RRM (RNA recognition motif) domains.
  • Post-translational
    modifications

    Phosphorylated. Its phosphorylation status increases in senescent cells.
  • Cellular localization

    Nucleus. Cytoplasm. RNA-binding activity is detected in both nuclear and cytoplasmic compartments.
  • Information by UniProt
  • Database links

  • Alternative names

    • 50 kDa Nuclear polyadenylated RNA binding protein antibody
    • 50 kDa nuclear polyadenylated RNA-binding protein antibody
    • Bruno like 2 antibody
    • bruno like protein 2 antibody
    • Bruno-like protein 2 antibody
    • BRUNOL 2 antibody
    • BRUNOL2 antibody
    • CELF 1 antibody
    • CELF-1 antibody
    • celf1 antibody
    • CELF1 CUGBP, Elav like family member 1 antibody
    • CELF1_HUMAN antibody
    • CUG BP and ETR 3 like factor 1 antibody
    • CUG BP antibody
    • CUG BP1 antibody
    • CUG RNA binding protein antibody
    • CUG triplet repeat RNA binding protein 1 antibody
    • CUG triplet repeat RNA-binding protein 1 antibody
    • CUG-BP antibody
    • CUG-BP- and ETR-3-like factor 1 antibody
    • CUG-BP1 antibody
    • CUGBP 1 antibody
    • CUGBP and ETR3 like factor 1 antibody
    • CUGBP antibody
    • CUGBP Elav like family member 1 antibody
    • CUGBP Elav-like family member 1 antibody
    • CUGBP1 antibody
    • Cytidine uridine guanosine binding protein 1 antibody
    • Deadenylation factor CUG BP antibody
    • Deadenylation factor CUG-BP antibody
    • Deadenylation factor CUGBP antibody
    • EDEN BP antibody
    • EDEN BP homolog antibody
    • EDEN-BP antibody
    • EDEN-BP homolog antibody
    • embryo deadenylation element binding protein antibody
    • embryo deadenylation element binding protein homolog antibody
    • Embryo deadenylation element-binding protein homolog antibody
    • hNab 50 antibody
    • hNab50 antibody
    • NAB 50 antibody
    • NAB50 antibody
    • NAPOR antibody
    • Nuclear polyadenylated RNA binding protein 50 kD antibody
    • Nuclear polyadenylated RNA binding protein antibody
    • RNA binding protein BRUNOL 2 antibody
    • RNA binding protein BRUNOL2 antibody
    • RNA-binding protein BRUNOL-2 antibody
    see all

Images

  • All lanes : Anti-CUG-BP1 antibody [EPR8298(B)] (ab129115) at 1/1000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : SH-SY5Y cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Standard HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 52 kDa
    Observed band size: 52 kDa

  • Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD

References

This product has been referenced in:

  • Saul MJ  et al. miR-574-5p as RNA decoy for CUGBP1 stimulates human lung tumor growth by mPGES-1 induction. FASEB J 33:6933-6947 (2019). Read more (PubMed: 30922080) »
  • Yuan T  et al. Low-density lipoprotein receptor-related protein 6 regulates alternative pre-mRNA splicing. J Cell Mol Med 22:4653-4663 (2018). Read more (PubMed: 30070011) »
See all 4 Publications for this product

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