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  1. Link

    cug-bp1-antibody-epr8298b-ab129115.pdf

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Epigenetics and Nuclear Signaling DNA / RNA RNA Processing
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Validated using a knockout cell lineRecombinantRabMAb

Recombinant Anti-CUG-BP1 antibody [EPR8298(B)] (ab129115)

  • Datasheet
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Western blot - Anti-CUG-BP1 antibody [EPR8298(B)] (ab129115)
  • Western blot - Anti-CUG-BP1 antibody [EPR8298(B)] (ab129115)
  • OI-RD Scanning - Anti-CUG-BP1 antibody [EPR8298(B)] (ab129115)
  • Anti-CUG-BP1 antibody [EPR8298(B)] (ab129115)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR8298(B)] to CUG-BP1
  • Suitable for: WB
  • Knockout validated
  • Reacts with: Human

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Overview

  • Product name

    Anti-CUG-BP1 antibody [EPR8298(B)]
    See all CUG-BP1 primary antibodies
  • Description

    Rabbit monoclonal [EPR8298(B)] to CUG-BP1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide within Human CUG-BP1 aa 1-100 (N terminal). The exact sequence is proprietary.
    Database link: Q92879

  • Positive control

    • WB: HEK-293T, HeLa and SH-SY5Y cell lysates.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Dissociation constant (KD)

    KD = 3.76 x 10 -10 M
    Learn more about KD
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR8298(B)
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • RNA Processing
    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • Translation
    • Regulation
    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • RNA Processing
    • Splicing
    • Neuroscience
    • Neurology process
    • Neurodegenerative disease
    • Other
    • Epigenetics and Nuclear Signaling
    • Chromatin Binding Proteins
    • DNA / RNA binding
    • Epigenetics and Nuclear Signaling
    • RNAi
    • Eukaryotic Initiation factors (eIF's)

Associated products

  • Alternative Versions

    • Alexa Fluor® 488 Anti-CUG-BP1 antibody [EPR8298(B)] (ab203844)
    • Alexa Fluor® 647 Anti-CUG-BP1 antibody [EPR8298(B)] (ab203845)
    • Anti-CUG-BP1 antibody [EPR8298(B)] - BSA and Azide free (ab240044)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • KO cell lines

    • Human CELF1 (CUG-BP1) knockout HEK-293T cell line (ab266086)
  • KO cell lysates

    • Human CELF1 (CUG-BP1) knockout HEK-293T cell lysate (ab257390)
  • Positive Controls

    • HeLa whole cell lysate (ab29545)
  • Recombinant Protein

    • Recombinant Human CUG-BP1 protein (ab153111)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab129115 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/1000 - 1/10000. Detects a band of approximately 52 kDa (predicted molecular weight: 52 kDa).
Notes
WB
1/1000 - 1/10000. Detects a band of approximately 52 kDa (predicted molecular weight: 52 kDa).

Target

  • Function

    RNA-binding protein implicated in the regulation of several post-transcriptional events. Involved in pre-mRNA alternative splicing, mRNA translation and stability. Mediates exon inclusion and/or exclusion in pre-mRNA that are subject to tissue-specific and developmentally regulated alternative splicing. Specifically activates exon 5 inclusion of cardiac isoforms of TNNT2 during heart remodeling at the juvenile to adult transition. Acts as both an activator and repressor of a pair of coregulated exons: promotes inclusion of the smooth muscle (SM) exon but exclusion of the non-muscle (NM) exon in actinin pre-mRNAs. Activates SM exon 5 inclusion by antagonizing the repressive effect of PTB. Promotes exclusion of exon 11 of the INSR pre-mRNA. Inhibits, together with HNRNPH1, insulin receptor (IR) pre-mRNA exon 11 inclusion in myoblast. Increases translation and controls the choice of translation initiation codon of CEBPB mRNA. Increases mRNA translation of CEBPB in aging liver (By similarity). Increases translation of CDKN1A mRNA by antagonizing the repressive effect of CALR3. Mediates rapid cytoplasmic mRNA deadenylation. Recruits the deadenylase PARN to the poly(A) tail of EDEN-containing mRNAs to promote their deadenylation. Required for completion of spermatogenesis (By similarity). Binds to (CUG)n triplet repeats in the 3'-UTR of transcripts such as DMPK and to Bruno response elements (BREs). Binds to muscle-specific splicing enhancer (MSE) intronic sites flanking the alternative exon 5 of TNNT2 pre-mRNA. Binds to AU-rich sequences (AREs or EDEN-like) localized in the 3'-UTR of JUN and FOS mRNAs. Binds to the IR RNA. Binds to the 5'-region of CDKN1A and CEBPB mRNAs. Binds with the 5'-region of CEBPB mRNA in aging liver.
  • Tissue specificity

    Ubiquitous.
  • Sequence similarities

    Belongs to the CELF/BRUNOL family.
    Contains 3 RRM (RNA recognition motif) domains.
  • Post-translational
    modifications

    Phosphorylated. Its phosphorylation status increases in senescent cells.
  • Cellular localization

    Nucleus. Cytoplasm. RNA-binding activity is detected in both nuclear and cytoplasmic compartments.
  • Target information above from: UniProt accession Q92879 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 10658 Human
    • Entrez Gene: 13046 Mouse
    • Entrez Gene: 362160 Rat
    • Omim: 601074 Human
    • SwissProt: Q92879 Human
    • SwissProt: P28659 Mouse
    • SwissProt: Q4QQT3 Rat
    • Unigene: 595333 Human
    • Unigene: 29495 Mouse
    • Unigene: 393354 Mouse
    • Unigene: 22432 Rat
    see all
  • Alternative names

    • 50 kDa Nuclear polyadenylated RNA binding protein antibody
    • 50 kDa nuclear polyadenylated RNA-binding protein antibody
    • Bruno like 2 antibody
    • bruno like protein 2 antibody
    • Bruno-like protein 2 antibody
    • BRUNOL 2 antibody
    • BRUNOL2 antibody
    • CELF 1 antibody
    • CELF-1 antibody
    • celf1 antibody
    • CELF1 CUGBP, Elav like family member 1 antibody
    • CELF1_HUMAN antibody
    • CUG BP and ETR 3 like factor 1 antibody
    • CUG BP antibody
    • CUG BP1 antibody
    • CUG RNA binding protein antibody
    • CUG triplet repeat RNA binding protein 1 antibody
    • CUG triplet repeat RNA-binding protein 1 antibody
    • CUG-BP antibody
    • CUG-BP- and ETR-3-like factor 1 antibody
    • CUG-BP1 antibody
    • CUGBP 1 antibody
    • CUGBP and ETR3 like factor 1 antibody
    • CUGBP antibody
    • CUGBP Elav like family member 1 antibody
    • CUGBP Elav-like family member 1 antibody
    • CUGBP1 antibody
    • Cytidine uridine guanosine binding protein 1 antibody
    • Deadenylation factor CUG BP antibody
    • Deadenylation factor CUG-BP antibody
    • Deadenylation factor CUGBP antibody
    • EDEN BP antibody
    • EDEN BP homolog antibody
    • EDEN-BP antibody
    • EDEN-BP homolog antibody
    • embryo deadenylation element binding protein antibody
    • embryo deadenylation element binding protein homolog antibody
    • Embryo deadenylation element-binding protein homolog antibody
    • hNab 50 antibody
    • hNab50 antibody
    • NAB 50 antibody
    • NAB50 antibody
    • NAPOR antibody
    • Nuclear polyadenylated RNA binding protein 50 kD antibody
    • Nuclear polyadenylated RNA binding protein antibody
    • RNA binding protein BRUNOL 2 antibody
    • RNA binding protein BRUNOL2 antibody
    • RNA-binding protein BRUNOL-2 antibody
    see all

Images

  • Western blot - Anti-CUG-BP1 antibody [EPR8298(B)] (ab129115)
    Western blot - Anti-CUG-BP1 antibody [EPR8298(B)] (ab129115)
    All lanes : Anti-CUG-BP1 antibody [EPR8298(B)] (ab129115) at 1/1000 dilution

    Lane 1 : Wild-type HEK293T cell lysate
    Lane 2 : CELF1 knockout HEK293T cell lysate
    Lane 3 : HeLa cell lysate
    Lane 4 : Human brain tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution

    Predicted band size: 52 kDa
    Observed band size: 52 kDa



    Lanes 1-4: Merged signal (red and green). Green - ab129115 observed at 52 kDa. Red - loading control ab8245 observed at 36 kDa.

    ab129115 Anti-CUG-BP1 antibody [EPR8298(B)] was shown to specifically react with CUG-BP1 in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266086 (knockout cell lysate ab257390) was used. Wild-type and CUG-BP1 knockout samples were subjected to SDS-PAGE. ab129115 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

     

     

  • Western blot - Anti-CUG-BP1 antibody [EPR8298(B)] (ab129115)
    Western blot - Anti-CUG-BP1 antibody [EPR8298(B)] (ab129115)
    All lanes : Anti-CUG-BP1 antibody [EPR8298(B)] (ab129115) at 1/1000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : SH-SY5Y cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Standard HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 52 kDa
    Observed band size: 52 kDa

  • OI-RD Scanning - Anti-CUG-BP1 antibody [EPR8298(B)] (ab129115)
    OI-RD Scanning - Anti-CUG-BP1 antibody [EPR8298(B)] (ab129115)
    Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD
  • Anti-CUG-BP1 antibody [EPR8298(B)] (ab129115)
    Anti-CUG-BP1 antibody [EPR8298(B)] (ab129115)

Protocols

  • Western blot protocols

Click here to view the general protocols

Datasheets and documents

  • Datasheet download

    Download

References (6)

Publishing research using ab129115? Please let us know so that we can cite the reference in this datasheet.

ab129115 has been referenced in 6 publications.

  • Handzlik MK  et al. Potential role for pyruvate kinase M2 in the regulation of murine cardiac glycolytic flux during in vivo chronic hypoxia. Biosci Rep 41:N/A (2021). PubMed: 33973628
  • Pandya-Jones A  et al. A protein assembly mediates Xist localization and gene silencing. Nature 587:145-151 (2020). PubMed: 32908311
  • Saul MJ  et al. miR-574-5p as RNA decoy for CUGBP1 stimulates human lung tumor growth by mPGES-1 induction. FASEB J 33:6933-6947 (2019). PubMed: 30922080
  • Yuan T  et al. Low-density lipoprotein receptor-related protein 6 regulates alternative pre-mRNA splicing. J Cell Mol Med 22:4653-4663 (2018). PubMed: 30070011
  • Hou L  et al. Nano-delivery of fraxinellone remodels tumor microenvironment and facilitates therapeutic vaccination in desmoplastic melanoma. Theranostics 8:3781-3796 (2018). PubMed: 30083259
  • Wu X  et al. CUG-binding protein 1 regulates HSC activation and liver fibrogenesis. Nat Commun 7:13498 (2016). IHC . PubMed: 27853137

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