Recombinant Anti-CUG-BP1 antibody [EPR8298(B)] (ab129115)
![Western blot - Anti-CUG-BP1 antibody [EPR8298(B)] (ab129115)](https://www.abcam.com/ps/products/129/ab129115/Images/ab129115-389424-anti-cug-bp1-antibody-epr8298b-western-blot-human-knockout-lysate.jpg "Western blot - Anti-CUG-BP1 antibody [EPR8298(B)] (ab129115)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR8298(B)] to CUG-BP1
- Suitable for: WB
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-CUG-BP1 antibody [EPR8298(B)]
See all CUG-BP1 primary antibodies -
Description
Rabbit monoclonal [EPR8298(B)] to CUG-BP1 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat
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Immunogen
Synthetic peptide within Human CUG-BP1 aa 1-100 (N terminal). The exact sequence is proprietary.
Database link: Q92879 -
Positive control
- WB: HEK-293T, HeLa and SH-SY5Y cell lysates.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Dissociation constant (KD)
KD = 3.76 x 10 -10 M Learn more about KD -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR8298(B) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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KO cell lines
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KO cell lysates
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab129115 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000 - 1/10000. Detects a band of approximately 52 kDa (predicted molecular weight: 52 kDa).
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| Notes |
|---|
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WB
1/1000 - 1/10000. Detects a band of approximately 52 kDa (predicted molecular weight: 52 kDa). |
Target
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Function
RNA-binding protein implicated in the regulation of several post-transcriptional events. Involved in pre-mRNA alternative splicing, mRNA translation and stability. Mediates exon inclusion and/or exclusion in pre-mRNA that are subject to tissue-specific and developmentally regulated alternative splicing. Specifically activates exon 5 inclusion of cardiac isoforms of TNNT2 during heart remodeling at the juvenile to adult transition. Acts as both an activator and repressor of a pair of coregulated exons: promotes inclusion of the smooth muscle (SM) exon but exclusion of the non-muscle (NM) exon in actinin pre-mRNAs. Activates SM exon 5 inclusion by antagonizing the repressive effect of PTB. Promotes exclusion of exon 11 of the INSR pre-mRNA. Inhibits, together with HNRNPH1, insulin receptor (IR) pre-mRNA exon 11 inclusion in myoblast. Increases translation and controls the choice of translation initiation codon of CEBPB mRNA. Increases mRNA translation of CEBPB in aging liver (By similarity). Increases translation of CDKN1A mRNA by antagonizing the repressive effect of CALR3. Mediates rapid cytoplasmic mRNA deadenylation. Recruits the deadenylase PARN to the poly(A) tail of EDEN-containing mRNAs to promote their deadenylation. Required for completion of spermatogenesis (By similarity). Binds to (CUG)n triplet repeats in the 3'-UTR of transcripts such as DMPK and to Bruno response elements (BREs). Binds to muscle-specific splicing enhancer (MSE) intronic sites flanking the alternative exon 5 of TNNT2 pre-mRNA. Binds to AU-rich sequences (AREs or EDEN-like) localized in the 3'-UTR of JUN and FOS mRNAs. Binds to the IR RNA. Binds to the 5'-region of CDKN1A and CEBPB mRNAs. Binds with the 5'-region of CEBPB mRNA in aging liver. -
Tissue specificity
Ubiquitous. -
Sequence similarities
Belongs to the CELF/BRUNOL family.
Contains 3 RRM (RNA recognition motif) domains. -
Post-translational
modificationsPhosphorylated. Its phosphorylation status increases in senescent cells. -
Cellular localization
Nucleus. Cytoplasm. RNA-binding activity is detected in both nuclear and cytoplasmic compartments. - Information by UniProt
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Database links
- Entrez Gene: 10658 Human
- Entrez Gene: 13046 Mouse
- Entrez Gene: 362160 Rat
- Omim: 601074 Human
- SwissProt: Q92879 Human
- SwissProt: P28659 Mouse
- SwissProt: Q4QQT3 Rat
- Unigene: 595333 Human
see all -
Alternative names
- 50 kDa Nuclear polyadenylated RNA binding protein antibody
- 50 kDa nuclear polyadenylated RNA-binding protein antibody
- Bruno like 2 antibody
see all
Images
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Western blot - Anti-CUG-BP1 antibody [EPR8298(B)] (ab129115)All lanes : Anti-CUG-BP1 antibody [EPR8298(B)] (ab129115) at 1/1000 dilution
Lane 1 : Wild-type HEK293T cell lysate
Lane 2 : CELF1 knockout HEK293T cell lysate
Lane 3 : HeLa cell lysate
Lane 4 : Human brain tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution
Predicted band size: 52 kDa
Observed band size: 52 kDaLanes 1-4: Merged signal (red and green). Green - ab129115 observed at 52 kDa. Red - loading control ab8245 observed at 36 kDa.
ab129115 Anti-CUG-BP1 antibody [EPR8298(B)] was shown to specifically react with CUG-BP1 in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266086 (knockout cell lysate ab257390) was used. Wild-type and CUG-BP1 knockout samples were subjected to SDS-PAGE. ab129115 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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![Western blot - Anti-CUG-BP1 antibody [EPR8298(B)] (ab129115)](https://www.abcam.com/ps/products/129/ab129115/Images/ab129115-143126-anti-cug-bp1-antibody-epr8298b-western-blot.jpg)
Western blot - Anti-CUG-BP1 antibody [EPR8298(B)] (ab129115)All lanes : Anti-CUG-BP1 antibody [EPR8298(B)] (ab129115) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : SH-SY5Y cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Standard HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 52 kDa
Observed band size: 52 kDa -
![OI-RD Scanning - Anti-CUG-BP1 antibody [EPR8298(B)] (ab129115)](https://www.abcam.com/ps/products/129/ab129115/Images/ab129115-143125-anti-cug-bp1-antibody-epr8298b.jpg)
OI-RD Scanning - Anti-CUG-BP1 antibody [EPR8298(B)] (ab129115) -
![Anti-CUG-BP1 antibody [EPR8298(B)] (ab129115)](https://www.abcam.com/ps/products/129/ab129115/Images/ab129115-4-benefits-of-recombinant-antibodies.png)
Anti-CUG-BP1 antibody [EPR8298(B)] (ab129115)
Datasheets and documents
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Datasheet download
References (6)
ab129115 has been referenced in 6 publications.
- Handzlik MK et al. Potential role for pyruvate kinase M2 in the regulation of murine cardiac glycolytic flux during in vivo chronic hypoxia. Biosci Rep 41:N/A (2021). PubMed: 33973628
- Pandya-Jones A et al. A protein assembly mediates Xist localization and gene silencing. Nature 587:145-151 (2020). PubMed: 32908311
- Saul MJ et al. miR-574-5p as RNA decoy for CUGBP1 stimulates human lung tumor growth by mPGES-1 induction. FASEB J 33:6933-6947 (2019). PubMed: 30922080
- Yuan T et al. Low-density lipoprotein receptor-related protein 6 regulates alternative pre-mRNA splicing. J Cell Mol Med 22:4653-4663 (2018). PubMed: 30070011
- Hou L et al. Nano-delivery of fraxinellone remodels tumor microenvironment and facilitates therapeutic vaccination in desmoplastic melanoma. Theranostics 8:3781-3796 (2018). PubMed: 30083259
- Wu X et al. CUG-binding protein 1 regulates HSC activation and liver fibrogenesis. Nat Commun 7:13498 (2016). IHC . PubMed: 27853137
