Overview

  • Product name

    Anti-CUL-4A + CUL-4B antibody [EPR3200]
    See all CUL-4A + CUL-4B primary antibodies
  • Description

    Rabbit monoclonal [EPR3200] to CUL-4A + CUL-4B
  • Host species

    Rabbit
  • Specificity

    Due to the close homology between Cullin 4A and 4B this antibody is likely to react with both forms.
  • Tested applications

    Suitable for: WB, IHC-P, Flow Cytmore details
    Unsuitable for: ICC/IF or IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human CUL-4A + CUL-4B aa 850-950. The exact sequence is proprietary.

  • Positive control

    • Fetal uterus, HeLa, A375 and fetal testis lysates and human brain tissue
  • General notes

     

     

     This product was previously labelled as Cullin 4a, Cullin 4A/B

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab76470 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Detects a band of approximately 88, 102 kDa (predicted molecular weight: 102 kDa).
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Use of HRP-conjugated or polymerized HRP secondary antibodies, stronger signals have been found using the polymerized HRP secondary.

Flow Cyt 1/60.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

  • Application notes
    Is unsuitable for ICC/IF or IP.
  • Target

    • Relevance

      Core component of multiple cullin-RING-based E3 ubiquitin-protein ligase complexes which mediate the ubiquitination and subsequent proteasomal degradation of target proteins. As a scaffold protein may contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. The E3 ubiquitin-protein ligase activity of the complex is dependent on the neddylation of the cullin subunit and is inhibited by the association of the deneddylated cullin subunit with TIP120A/CAND1. The functional specificity of the E3 ubiquitin-protein ligase complex depends on the variable substrate recognition component.
    • Database links

    Images

    • All lanes : Anti-CUL-4A + CUL-4B antibody [EPR3200] (ab76470) at 1/5000 dilution

      Lane 1 : Fetal uterus lysate
      Lane 2 : Hela lysate
      Lane 3 : A375 lysate
      Lane 4 : Fetal testis lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 102 kDa
      Observed band size: 102,88 kDa
      why is the actual band size different from the predicted?

    • ab76470 at 1/100 dilution staining Cullin 4B in human brain tissue, using a HRP/AP polymerized secondary antibody.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    References

    This product has been referenced in:

    • Hou L  et al. Restriction of hepatitis B virus replication by c-Abl-induced proteasomal degradation of the viral polymerase. Sci Adv 5:eaau7130 (2019). Read more (PubMed: 30775435) »
    • Song T  et al. CRL4 antagonizes SCFFbxo7-mediated turnover of cereblon and BK channel to regulate learning and memory. PLoS Genet 14:e1007165 (2018). Read more (PubMed: 29370161) »
    See all 2 Publications for this product

    Customer reviews and Q&As

    1-2 of 2 Abreviews or Q&A

    Answer

    Thank you for your enquiry regading ab76470.
    I can confirm that the sequence we used to raise Anti-Cullin 4A/B antibody maps to a region that is conserved between CUL4A and B.
    In addition, we observe 2 bands (at 88 and at 102 kDa) in samples that express both isoforms.
    - Have you tested a sample that is known to express CULA, such as A375 or HeLa?
    I have attached an additional image showing the CUL4A expression in mouse and rat samples to share with you.
    If you need any further assistance in the future, please do not hesitate to contact me.

    Read More

    Answer

    Thank you for your inquiry.
    Unfortunately, we are not able to release the immunogen sequence for this particular antibody as it is proprietary information.
    We aim to provide as much information as possible to our customers, so I am sorry that this has not been possible on this occasion.
    Should you have any further questions, please do not hesitate to contact us.

    Read More

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