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Synthetic peptide within Human Cullin 2/ CUL-2 aa 1-100. The exact sequence is proprietary.
Database link: Q13617
Our Abpromise guarantee covers the use of ab166917 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Predicted molecular weight: 87 kDa.|
|ICC/IF||1/100 - 1/250.|
|IP||1/10 - 1/100.|
Immunofluorescent analysis of 293T cells labeling Cullin 2 / CUL-2 with ab166917 at 1/100.
Flow cytometry analysis of 293T (Human embryonic kidney epithelial cell) cells labeling Cullin 2 / CUL-2 (red) with ab166917 at a 1/100 dilution. Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. A goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a 1/2000 dilution. Black - Rabbit monoclonal IgG (ab172730). Blue (unlabeled control) - Cells without incubation with the primary and secondary antibodies.
Western blot analysis of an immunoprecipitation pellet from Raji cell lysate labeling Cullin 2 / CUL-2 with ab166917 which was used at 1/10 dilution for immunoprecipitation.
Secondary antibody used was an HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.
Blocking and Diluting buffer: 5% NFDM/TBST
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"