Recombinant Anti-Cullin 2/CUL-2 antibody [EPR3104(2)] (ab166917)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR3104(2)] to Cullin 2/CUL-2
- Suitable for: Flow Cyt (Intra), WB, IP
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-Cullin 2/CUL-2 antibody [EPR3104(2)]
See all Cullin 2/CUL-2 primary antibodies -
Description
Rabbit monoclonal [EPR3104(2)] to Cullin 2/CUL-2 -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), WB, IPmore details
Unsuitable for: ICC/IF or IHC-P -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide within Human Cullin 2/CUL-2 aa 1-100. The exact sequence is proprietary.
Database link: Q13617 -
Positive control
- WB: PC3, 293T, HeLa, Raji cell lysates; mouse and rat brain, heart, kidney and spleen lysates. Flow Cyt (intra): 293T cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR3104(2) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab166917 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
Flow Cyt (Intra) |
1/100.
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WB | (2) |
1/1000 - 1/10000. Predicted molecular weight: 87 kDa.
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IP | (1) |
1/10 - 1/100.
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Notes |
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Flow Cyt (Intra)
1/100. |
WB
1/1000 - 1/10000. Predicted molecular weight: 87 kDa. |
IP
1/10 - 1/100. |
Target
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Function
Core component of multiple cullin-RING-based ECS (ElonginB/C-CUL2/5-SOCS-box protein) E3 ubiquitin-protein ligase complexes, which mediate the ubiquitination of target proteins. May serve as a rigid scaffold in the complex and may contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. The E3 ubiquitin-protein ligase activity of the complex is dependent on the neddylation of the cullin subunit and is inhibited by the association of the deneddylated cullin subunit with TIP120A/CAND1 (By similarity). The functional specificity of the ECS complex depends on the substrate recognition component. ECS(VHL) mediates the ubiquitination of hypoxia-inducible factor (HIF). -
Pathway
Protein modification; protein ubiquitination. -
Sequence similarities
Belongs to the cullin family. -
Post-translational
modificationsCBC(VHL) complex formation seems to promote neddylation. Deneddylated via its interaction with the COP9 signalosome (CSN) complex. - Information by UniProt
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Database links
- Entrez Gene: 8453 Human
- Entrez Gene: 71745 Mouse
- Entrez Gene: 361258 Rat
- Omim: 603135 Human
- SwissProt: Q13617 Human
- SwissProt: Q9D4H8 Mouse
- Unigene: 82919 Human
- Unigene: 291707 Mouse
see all -
Alternative names
- Cul2 antibody
- CUL 2 antibody
- CUL-2 antibody
see all
Images
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All lanes : Anti-Cullin 2/CUL-2 antibody [EPR3104(2)] (ab166917) at 1/1000 dilution
Lane 1 : PC3 cell lysate
Lane 2 : 293T cell lysate
Lane 3 : HeLa cell lysate
Lane 4 : Raji cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 87 kDa -
Intracellular Flow Cytometry analysis of 293T (Human embryonic kidney epithelial cell) cells labeling Cullin 2/CUL-2 (red) with ab166917 at a 1/100 dilution. Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. A goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a 1/2000 dilution. Black - Rabbit monoclonal IgG (ab172730). Blue (unlabeled control) - Cells without incubation with the primary and secondary antibodies.
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Western blot analysis of an immunoprecipitation pellet from Raji cell lysate labeling Cullin 2/CUL-2 with ab166917 which was used at 1/10 dilution for immunoprecipitation.
Secondary antibody used was an HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG. -
All lanes : Anti-Cullin 2/CUL-2 antibody [EPR3104(2)] (ab166917) at 1/2000 dilution
Lane 1 : Mouse brain lysate
Lane 2 : Mouse heart lysate
Lane 3 : Mouse kidney lysate
Lane 4 : Mouse spleen lysate
Lane 5 : Rat brain lysate
Lane 6 : Rat heart lysate
Lane 7 : Rat kidney lysate
Lane 8 : Rat spleen lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 87 kDa
Exposure time: 3 minutesBlocking and Diluting buffer: 5% NFDM/TBST
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (20)
ab166917 has been referenced in 20 publications.
- Zhou Q et al. Neddylation inhibition induces glutamine uptake and metabolism by targeting CRL3SPOP E3 ligase in cancer cells. Nat Commun 13:3034 (2022). PubMed: 35641493
- Wang Q et al. Post-translational control of beige fat biogenesis by PRDM16 stabilization. Nature 609:151-158 (2022). PubMed: 35978186
- Yin L et al. Early growth response-1 is a new substrate of the GSK3β-FBXW7 axis. Neoplasia 34:100839 (2022). PubMed: 36240645
- Xu T et al. A small molecule inhibitor of the UBE2F-CRL5 axis induces apoptosis and radiosensitization in lung cancer. Signal Transduct Target Ther 7:354 (2022). PubMed: 36253371
- Zhang W et al. Tumor-associated antigen Prame targets tumor suppressor p14/ARF for degradation as the receptor protein of CRL2Prame complex. Cell Death Differ 28:1926-1940 (2021). PubMed: 33504946