Recombinant
RabMAb

Anti-Cullin 2 antibody [EPR3104(2)] (ab166917)

Overview

  • Product name
    Anti-Cullin 2 antibody [EPR3104(2)]
    See all Cullin 2 primary antibodies
  • Description
    Rabbit monoclonal [EPR3104(2)] to Cullin 2
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, ICC/IF, IP, Flow Cytmore details
    Unsuitable for: IHC-P
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human Cullin 2 aa 1-100. The exact sequence is proprietary.
    Database link: Q13617

  • Positive control
    • WB: PC3, 293T, HeLa, Raji cell lysates; mouse and rat brain, heart, kidney and spleen lysates. ICC/IF: 293T cells. Flow Cyt: 293T cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab166917 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 87 kDa.
ICC/IF 1/100 - 1/250.
IP 1/10 - 1/100.
Flow Cyt 1/100.
  • Application notes
    Is unsuitable for IHC-P.
  • Target

    • Function
      Core component of multiple cullin-RING-based ECS (ElonginB/C-CUL2/5-SOCS-box protein) E3 ubiquitin-protein ligase complexes, which mediate the ubiquitination of target proteins. May serve as a rigid scaffold in the complex and may contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. The E3 ubiquitin-protein ligase activity of the complex is dependent on the neddylation of the cullin subunit and is inhibited by the association of the deneddylated cullin subunit with TIP120A/CAND1 (By similarity). The functional specificity of the ECS complex depends on the substrate recognition component. ECS(VHL) mediates the ubiquitination of hypoxia-inducible factor (HIF).
    • Pathway
      Protein modification; protein ubiquitination.
    • Sequence similarities
      Belongs to the cullin family.
    • Post-translational
      modifications
      CBC(VHL) complex formation seems to promote neddylation. Deneddylated via its interaction with the COP9 signalosome (CSN) complex.
    • Information by UniProt
    • Database links
    • Alternative names
      • Cul2 antibody
      • CUL 2 antibody
      • CUL-2 antibody
      • CUL2 antibody
      • CUL2_HUMAN antibody
      • cullin 2 antibody
      • cullin homolog 2 antibody
      • Cullin-2 antibody
      • MGC131970 antibody
      see all

    Images

    • All lanes : Anti-Cullin 2 antibody [EPR3104(2)] (ab166917) at 1/1000 dilution

      Lane 1 : PC3 cell lysate
      Lane 2 : 293T cell lysate
      Lane 3 : HeLa cell lysate
      Lane 4 : Raji cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 87 kDa

    • Immunofluorescent analysis of 293T cells labeling Cullin 2 with ab166917 at 1/100.
    • Flow cytometry analysis of 293T (Human embryonic kidney epithelial cell) cells labeling Cullin 2 (red) with ab166917 at a 1/100 dilution. Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. A goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a 1/2000 dilution. Black - Rabbit monoclonal IgG (ab172730). Blue (unlabeled control) - Cells without incubation with the primary and secondary antibodies.

    • Western blot analysis of an immunoprecipitation pellet from Raji cell lysate labeling Cullin 2 with ab166917 which was used at 1/10 dilution for immunoprecipitation.
      Secondary antibody used was an HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.
    • All lanes : Anti-Cullin 2 antibody [EPR3104(2)] (ab166917) at 1/2000 dilution

      Lane 1 : Mouse brain lysate
      Lane 2 : Mouse heart lysate
      Lane 3 : Mouse kidney lysate
      Lane 4 : Mouse spleen lysate
      Lane 5 : Rat brain lysate
      Lane 6 : Rat heart lysate
      Lane 7 : Rat kidney lysate
      Lane 8 : Rat spleen lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 87 kDa


      Exposure time: 3 minutes


      Blocking and Diluting buffer: 5% NFDM/TBST

    References

    This product has been referenced in:

    See all 2 Publications for this product

    Customer reviews and Q&As

    Application
    Immunoprecipitation
    Sample
    Xenopus laevis Cell lysate - whole cell (Xenopus egg extracts)
    Total protein in input
    10 µg
    Immuno-precipitation step
    Protein A
    Specification
    Xenopus egg extracts
    Username

    Abcam user community

    Verified customer

    Submitted Jun 29 2018

    Application
    Western blot
    Sample
    Xenopus laevis Cell lysate - other (Interphase Xenopus egg extract)
    Gel Running Conditions
    Reduced Denaturing (4-20% Tris Glycine)
    Loading amount
    300 µg
    Specification
    Interphase Xenopus egg extract
    Blocking step
    Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 21°C
    Username

    Abcam user community

    Verified customer

    Submitted Mar 27 2018

    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (mouse embryonic stem cell)
    Gel Running Conditions
    Reduced Denaturing (10% gel)
    Loading amount
    100000 cells
    Specification
    mouse embryonic stem cell
    Blocking step
    Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 16°C
    Username

    Abcam user community

    Verified customer

    Submitted Aug 19 2015

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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