Key features and details
- Rabbit polyclonal to Cullin 2/CUL-2
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-Cullin 2/CUL-2 antibody
See all Cullin 2/CUL-2 primary antibodies
DescriptionRabbit polyclonal to Cullin 2/CUL-2
ab133180 detects endogenous levels of total Cullin 2/CUL-2 protein.
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Species reactivityReacts with: Mouse, Human
Synthetic peptide corresponding to Human Cullin 2/CUL-2 (C terminal).
Previously labelled as Cullin 2.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 0.88% Sodium chloride, 50% Glycerol
PBS is without Mg2+ and Ca2+
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab133180 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Predicted molecular weight: 87 kDa.|
|IHC-P||Use a concentration of 10 µg/ml.|
|ICC/IF||1/100 - 1/500.|
FunctionCore component of multiple cullin-RING-based ECS (ElonginB/C-CUL2/5-SOCS-box protein) E3 ubiquitin-protein ligase complexes, which mediate the ubiquitination of target proteins. May serve as a rigid scaffold in the complex and may contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. The E3 ubiquitin-protein ligase activity of the complex is dependent on the neddylation of the cullin subunit and is inhibited by the association of the deneddylated cullin subunit with TIP120A/CAND1 (By similarity). The functional specificity of the ECS complex depends on the substrate recognition component. ECS(VHL) mediates the ubiquitination of hypoxia-inducible factor (HIF).
PathwayProtein modification; protein ubiquitination.
Sequence similaritiesBelongs to the cullin family.
modificationsCBC(VHL) complex formation seems to promote neddylation. Deneddylated via its interaction with the COP9 signalosome (CSN) complex.
- Information by UniProt
- Cul2 antibody
- CUL 2 antibody
- CUL-2 antibody
All lanes : Anti-Cullin 2/CUL-2 antibody (ab133180) at 1/500 dilution
Lane 1 : LOVO cell extract
Lane 2 : LOVO cell extract with synthesized
Predicted band size: 87 kDa
Immunohistochemical analysis of paraffin embedded Human testis tissue labelling Cullin 2/CUL-2 with ab133180 antibody at a concentration of 10 µg/ml followed by biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.
Immunofluorescent analysis of NIH 3T3 cells labelling Cullin 2/CUL-2 with ab133180 antibody at a dilution of 1/100. The image on the right is treated with the synthesized peptide.
ab133180 has been referenced in 1 publication.
- Friend SF et al. The Discovery of a Reciprocal Relationship between Tyrosine-Kinase Signaling and Cullin Neddylation. PLoS One 8:e75200 (2013). Mouse . PubMed: 24124476