Overview

  • Product name

    Anti-Cullin 2/CUL-2 antibody
    See all Cullin 2/CUL-2 primary antibodies
  • Description

    Rabbit polyclonal to Cullin 2/CUL-2
  • Host species

    Rabbit
  • Specificity

    ab133180 detects endogenous levels of total Cullin 2/CUL-2 protein.

  • Tested applications

    Suitable for: ELISA, WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide corresponding to Human Cullin 2/CUL-2 (C terminal).

  • Positive control

    • LOVO cell extract; Human testis tissue; NIH 3T3 cells.
  • General notes

    Previously labelled as Cullin 2. 

Properties

Applications

Our Abpromise guarantee covers the use of ab133180 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/5000.
WB 1/500 - 1/1000. Predicted molecular weight: 87 kDa.
IHC-P Use a concentration of 10 µg/ml.
ICC/IF 1/100 - 1/500.

Target

  • Function

    Core component of multiple cullin-RING-based ECS (ElonginB/C-CUL2/5-SOCS-box protein) E3 ubiquitin-protein ligase complexes, which mediate the ubiquitination of target proteins. May serve as a rigid scaffold in the complex and may contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. The E3 ubiquitin-protein ligase activity of the complex is dependent on the neddylation of the cullin subunit and is inhibited by the association of the deneddylated cullin subunit with TIP120A/CAND1 (By similarity). The functional specificity of the ECS complex depends on the substrate recognition component. ECS(VHL) mediates the ubiquitination of hypoxia-inducible factor (HIF).
  • Pathway

    Protein modification; protein ubiquitination.
  • Sequence similarities

    Belongs to the cullin family.
  • Post-translational
    modifications

    CBC(VHL) complex formation seems to promote neddylation. Deneddylated via its interaction with the COP9 signalosome (CSN) complex.
  • Information by UniProt
  • Database links

  • Alternative names

    • Cul2 antibody
    • CUL 2 antibody
    • CUL-2 antibody
    • CUL2 antibody
    • CUL2_HUMAN antibody
    • cullin 2 antibody
    • cullin homolog 2 antibody
    • Cullin-2 antibody
    • MGC131970 antibody
    see all

Images

  • All lanes : Anti-Cullin 2/CUL-2 antibody (ab133180) at 1/500 dilution

    Lane 1 : LOVO cell extract
    Lane 2 : LOVO cell extract with synthesized
    peptide

    Predicted band size: 87 kDa

  • Immunohistochemical analysis of paraffin embedded Human testis tissue labelling Cullin 2/CUL-2 with ab133180 antibody at a concentration of 10 µg/ml followed by biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.

  • Immunofluorescent analysis of NIH 3T3 cells labelling Cullin 2/CUL-2 with ab133180 antibody at a dilution of 1/100. The image on the right is treated with the synthesized peptide.

References

This product has been referenced in:

  • Friend SF  et al. The Discovery of a Reciprocal Relationship between Tyrosine-Kinase Signaling and Cullin Neddylation. PLoS One 8:e75200 (2013). Mouse . Read more (PubMed: 24124476) »
See 1 Publication for this product

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