Recombinant
RabMAb

Recombinant Anti-Cullin 2/CUL-2 antibody [EPR3104(2)] - BSA and Azide free (ab240149)

Overview

  • Product name

    Anti-Cullin 2/CUL-2 antibody [EPR3104(2)] - BSA and Azide free
    See all Cullin 2/CUL-2 primary antibodies
  • Description

    Rabbit monoclonal [EPR3104(2)] to Cullin 2/CUL-2 - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IF, IP, Flow Cytmore details
    Unsuitable for: IHC-P
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human Cullin 2/CUL-2 aa 1-100. The exact sequence is proprietary.
    Database link: Q13617

  • General notes

    Ab240149 is the carrier-free version of ab166917. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab240149 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab240149 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Predicted molecular weight: 87 kDa.
ICC/IF Use at an assay dependent concentration.
IP Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration.
  • Application notes
    Is unsuitable for IHC-P.
  • Target

    • Function

      Core component of multiple cullin-RING-based ECS (ElonginB/C-CUL2/5-SOCS-box protein) E3 ubiquitin-protein ligase complexes, which mediate the ubiquitination of target proteins. May serve as a rigid scaffold in the complex and may contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. The E3 ubiquitin-protein ligase activity of the complex is dependent on the neddylation of the cullin subunit and is inhibited by the association of the deneddylated cullin subunit with TIP120A/CAND1 (By similarity). The functional specificity of the ECS complex depends on the substrate recognition component. ECS(VHL) mediates the ubiquitination of hypoxia-inducible factor (HIF).
    • Pathway

      Protein modification; protein ubiquitination.
    • Sequence similarities

      Belongs to the cullin family.
    • Post-translational
      modifications

      CBC(VHL) complex formation seems to promote neddylation. Deneddylated via its interaction with the COP9 signalosome (CSN) complex.
    • Information by UniProt
    • Database links

    • Alternative names

      • Cul2 antibody
      • CUL 2 antibody
      • CUL-2 antibody
      • CUL2 antibody
      • CUL2_HUMAN antibody
      • cullin 2 antibody
      • cullin homolog 2 antibody
      • Cullin-2 antibody
      • MGC131970 antibody
      see all

    Images

    • Flow cytometry analysis of 293T (Human embryonic kidney epithelial cell) cells labeling Cullin 2/CUL-2 (red) with ab166917 at a 1/100 dilution. Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. A goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a 1/2000 dilution. Black - Rabbit monoclonal IgG (ab172730). Blue (unlabeled control) - Cells without incubation with the primary and secondary antibodies.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab166917).

    • Immunofluorescent analysis of 293T cells labeling Cullin 2/CUL-2 with ab166917 at 1/100.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab166917).

    • Western blot analysis of an immunoprecipitation pellet from Raji cell lysate labeling Cullin 2/CUL-2 with ab166917 which was used at 1/10 dilution for immunoprecipitation.
      Secondary antibody used was an HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab166917).

    References

    ab240149 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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