Key features and details
- Rabbit polyclonal to Cullin 3/CUL-3
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Product nameAnti-Cullin 3/CUL-3 antibody
See all Cullin 3/CUL-3 primary antibodies
DescriptionRabbit polyclonal to Cullin 3/CUL-3
Tested applicationsSuitable for: WB, ICC/IFmore details
Species reactivityReacts with: Mouse, Rat, Human
Recombinant fragment corresponding to Human Cullin 3/CUL-3 aa 569-768.
KEDGSEVGVGGAQVTGSNTRKHILQVSTFQMTILMLFNNREKYTFEEIQQ ETDIPERELVRALQSLACGKPTQRVLTKEPKSKEIENGHIFTVNDQFTSK LHRVKIQTVAAKQGESDPERKETRQKVDDDRKHEIEAAIVRIMKSRKKMQ HNVLVAEVTQQLKARFLPSPVVIKKRIEGLIEREYLARTPEDRKVYTYVA
Database link: Q13618
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 50% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab194584 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/2000. Predicted molecular weight: 89 kDa.|
|ICC/IF||1/50 - 1/200.|
FunctionCore component of multiple cullin-RING-based BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complexes which mediate the ubiquitination and subsequent proteasomal degradation of target proteins. As a scaffold protein may contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. The E3 ubiquitin-protein ligase activity of the complex is dependent on the neddylation of the cullin subunit and is inhibited by the association of the deneddylated cullin subunit with TIP120A/CAND1 (By similarity). The functional specificity of the BCR complex depends on the BTB domain-containing protein as the susbstrate recognition component. BCR(SPOP) is involved in ubiquitination of BMI1/PCGF4, H2AFY and DAXX, and probably GLI2 or GLI3. BCR(KLHL9-KLHL13) controls the dynamic behavior of AURKB on mitotic chromosomes and thereby coordinates faithful mitotic progression and completion of cytokinesis. Involved in ubiquitination of cyclin E and of cyclin D1 (in vitro) thus involved in regulation of G1/S transition.
Tissue specificityWidely expressed.
PathwayProtein modification; protein ubiquitination.
Sequence similaritiesBelongs to the cullin family.
modificationsNeddylated. Attachment of NEDD8 is required for the E3 ubiquitin-protein ligase activity of the BCR complex. Deneddylated via its interaction with the COP9 signalosome (CSN) complex.
Cellular localizationNucleus. Golgi apparatus.
- Information by UniProt
- CUL 3 antibody
- Cul-3 antibody
- cul3 antibody
Anti-GPR37L1 antibody (ab194568) at 1/500 dilution + Jurkat cell lysate
Predicted band size: 89 kDa
Immunofluorescence analysis of HeLa cells labeling Cullin 3/CUL-3 with ab194584 at a 1/50 dilution.
Blue: DAPI for nuclear staining.
ab194584 has been referenced in 1 publication.
- Chung D et al. Inhibition of neddylation induces mitotic defects and alters MKLP1 accumulation at the midbody during cytokinesis. Cell Cycle 18:1135-1153 (2019). PubMed: 31057046