Recombinant
Recombinant Anti-Cullin 3/CUL-3 antibody [EPR3196Y] (ab75851)
- Datasheet
- References (5)
- Protocols
Overview
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Product name
Anti-Cullin 3/CUL-3 antibody [EPR3196Y]
See all Cullin 3/CUL-3 primary antibodies -
Description
Rabbit monoclonal [EPR3196Y] to Cullin 3/CUL-3 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IP, Flow Cytmore details
Unsuitable for: ICC or IHC-P -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide within Human Cullin 3/CUL-3 aa 750-850 (C terminal). The exact sequence is proprietary.
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Positive control
- HeLa, SH-SY5Y, PC12 and 3T3 cell lysates.
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR3196Y -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab75851 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/20000 - 1/100000. Predicted molecular weight: 89 kDa. | |
| IP | 1/40. | |
| Flow Cyt | 1/30 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
Application notes
Is unsuitable for ICC or IHC-P.
Target
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Function
Core component of multiple cullin-RING-based BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complexes which mediate the ubiquitination and subsequent proteasomal degradation of target proteins. As a scaffold protein may contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. The E3 ubiquitin-protein ligase activity of the complex is dependent on the neddylation of the cullin subunit and is inhibited by the association of the deneddylated cullin subunit with TIP120A/CAND1 (By similarity). The functional specificity of the BCR complex depends on the BTB domain-containing protein as the susbstrate recognition component. BCR(SPOP) is involved in ubiquitination of BMI1/PCGF4, H2AFY and DAXX, and probably GLI2 or GLI3. BCR(KLHL9-KLHL13) controls the dynamic behavior of AURKB on mitotic chromosomes and thereby coordinates faithful mitotic progression and completion of cytokinesis. Involved in ubiquitination of cyclin E and of cyclin D1 (in vitro) thus involved in regulation of G1/S transition. -
Tissue specificity
Widely expressed. -
Pathway
Protein modification; protein ubiquitination. -
Sequence similarities
Belongs to the cullin family. -
Post-translational
modificationsNeddylated. Attachment of NEDD8 is required for the E3 ubiquitin-protein ligase activity of the BCR complex. Deneddylated via its interaction with the COP9 signalosome (CSN) complex. -
Cellular localization
Nucleus. Golgi apparatus. - Information by UniProt
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Database links
- Entrez Gene: 8452 Human
- Entrez Gene: 26554 Mouse
- Entrez Gene: 301555 Rat
- Omim: 603136 Human
- SwissProt: Q13618 Human
- SwissProt: Q9JLV5 Mouse
- SwissProt: B5DF89 Rat
- Unigene: 372286 Human
see all -
Alternative names
- CUL 3 antibody
- Cul-3 antibody
- cul3 antibody
see all
Images
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![Flow Cytometry - Anti-Cullin 3/CUL-3 antibody [EPR3196Y] (ab75851)](https://www.abcam.com/ps/products/75/ab75851/Images/ab75851-2-ab75851FC.jpg)
Flow Cytometry - Anti-Cullin 3/CUL-3 antibody [EPR3196Y] (ab75851)Overlay histogram showing HeLa cells stained with ab75851 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab75851, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. -
![Western blot - Anti-Cullin 3/CUL-3 antibody [EPR3196Y] (ab75851)](https://www.abcam.com/ps/products/75/ab75851/Images/ab75851wb.jpg)
Western blot - Anti-Cullin 3/CUL-3 antibody [EPR3196Y] (ab75851)All lanes : Anti-Cullin 3/CUL-3 antibody [EPR3196Y] (ab75851) at 1/20000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : SH-SY5Y cell lysate
Lane 3 : PC12 cell lysate
Lane 4 : 3T3 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP-labeled goat anti-rabbit at 1/2000 dilution
Predicted band size: 89 kDa
Observed band size: 82,90 kDa why is the actual band size different from the predicted?
Datasheets and documents
References
This product has been referenced in:
- Jang SM et al. The replication initiation determinant protein (RepID) modulates replication by recruiting CUL4 to chromatin. Nat Commun 9:2782 (2018). Read more (PubMed: 30018425) »
- Yang N et al. Carnosic acid prevents dextran sulfate sodium-induced acute colitis associated with the regulation of the Keap1/Nrf2 pathway. Sci Rep 7:11036 (2017). Read more (PubMed: 28887507) »
