Recombinant Anti-Cullin 3/CUL-3 antibody [EPR3196Y] (ab75851)
![Flow Cytometry (Intracellular) - Anti-Cullin 3/CUL-3 antibody [EPR3196Y] (ab75851)](https://www.abcam.com/ps/products/75/ab75851/Images/ab75851-2-ab75851FC.jpg "Flow Cytometry (Intracellular) - Anti-Cullin 3/CUL-3 antibody [EPR3196Y] (ab75851)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR3196Y] to Cullin 3/CUL-3
- Suitable for: Flow Cyt (Intra), IP, WB
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-Cullin 3/CUL-3 antibody [EPR3196Y]
See all Cullin 3/CUL-3 primary antibodies -
Description
Rabbit monoclonal [EPR3196Y] to Cullin 3/CUL-3 -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), IP, WBmore details
Unsuitable for: ICC or IHC-P -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- HeLa, SH-SY5Y, PC12 and 3T3 cell lysates.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR3196Y -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab75851 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt (Intra) |
1/30 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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| IP |
Use at an assay dependent concentration.
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| WB | (1) |
1/20000 - 1/100000. Predicted molecular weight: 89 kDa.
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| Notes |
|---|
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Flow Cyt (Intra)
1/30 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
IP
Use at an assay dependent concentration. |
|
WB
1/20000 - 1/100000. Predicted molecular weight: 89 kDa. |
Target
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Function
Core component of multiple cullin-RING-based BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complexes which mediate the ubiquitination and subsequent proteasomal degradation of target proteins. As a scaffold protein may contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. The E3 ubiquitin-protein ligase activity of the complex is dependent on the neddylation of the cullin subunit and is inhibited by the association of the deneddylated cullin subunit with TIP120A/CAND1 (By similarity). The functional specificity of the BCR complex depends on the BTB domain-containing protein as the susbstrate recognition component. BCR(SPOP) is involved in ubiquitination of BMI1/PCGF4, H2AFY and DAXX, and probably GLI2 or GLI3. BCR(KLHL9-KLHL13) controls the dynamic behavior of AURKB on mitotic chromosomes and thereby coordinates faithful mitotic progression and completion of cytokinesis. Involved in ubiquitination of cyclin E and of cyclin D1 (in vitro) thus involved in regulation of G1/S transition. -
Tissue specificity
Widely expressed. -
Pathway
Protein modification; protein ubiquitination. -
Sequence similarities
Belongs to the cullin family. -
Post-translational
modificationsNeddylated. Attachment of NEDD8 is required for the E3 ubiquitin-protein ligase activity of the BCR complex. Deneddylated via its interaction with the COP9 signalosome (CSN) complex. -
Cellular localization
Nucleus. Golgi apparatus. - Information by UniProt
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Database links
- Entrez Gene: 8452 Human
- Entrez Gene: 26554 Mouse
- Entrez Gene: 301555 Rat
- Omim: 603136 Human
- SwissProt: Q13618 Human
- SwissProt: Q9JLV5 Mouse
- SwissProt: B5DF89 Rat
- Unigene: 372286 Human
see all -
Alternative names
- CUL 3 antibody
- Cul-3 antibody
- cul3 antibody
see all
Images
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Flow Cytometry (Intracellular) - Anti-Cullin 3/CUL-3 antibody [EPR3196Y] (ab75851)
Overlay histogram showing HeLa cells stained with ab75851 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab75851, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
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![Western blot - Anti-Cullin 3/CUL-3 antibody [EPR3196Y] (ab75851)](https://www.abcam.com/ps/products/75/ab75851/Images/ab75851wb.jpg)
Western blot - Anti-Cullin 3/CUL-3 antibody [EPR3196Y] (ab75851)All lanes : Anti-Cullin 3/CUL-3 antibody [EPR3196Y] (ab75851) at 1/20000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : SH-SY5Y cell lysate
Lane 3 : PC12 cell lysate
Lane 4 : 3T3 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP-labeled goat anti-rabbit at 1/2000 dilution
Predicted band size: 89 kDa
Observed band size: 82,90 kDa why is the actual band size different from the predicted? -
![Anti-Cullin 3/CUL-3 antibody [EPR3196Y] (ab75851)](https://www.abcam.com/ps/products/75/ab75851/Images/ab75851-3-benefits-of-recombinant-antibodies.png)
Anti-Cullin 3/CUL-3 antibody [EPR3196Y] (ab75851)
Datasheets and documents
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Datasheet download
References (10)
ab75851 has been referenced in 10 publications.
- Chen X et al. Long non-coding RNA XIST inhibits osteoblast differentiation and promotes osteoporosis via Nrf2 hyperactivation by targeting CUL3. Int J Mol Med 48:N/A (2021). PubMed: 34036379
- Lv W et al. Snail promotes prostate cancer migration by facilitating SPOP ubiquitination and degradation. Biochem Biophys Res Commun 529:799-804 (2020). PubMed: 32736710
- Füzesi-Levi MG et al. CSNAP, the smallest CSN subunit, modulates proteostasis through cullin-RING ubiquitin ligases. Cell Death Differ N/A:N/A (2019). PubMed: 31367012
- Zhou W et al. Potent 5-Cyano-6-phenyl-pyrimidin-Based Derivatives Targeting DCN1-UBE2M Interaction. J Med Chem 62:5382-5403 (2019). PubMed: 31157974
- Jang SM et al. The replication initiation determinant protein (RepID) modulates replication by recruiting CUL4 to chromatin. Nat Commun 9:2782 (2018). PubMed: 30018425
- Yang N et al. Carnosic acid prevents dextran sulfate sodium-induced acute colitis associated with the regulation of the Keap1/Nrf2 pathway. Sci Rep 7:11036 (2017). PubMed: 28887507
- Tan X et al. PtdIns(4,5)P2 signaling regulates ATG14 and autophagy. Proc Natl Acad Sci U S A 113:10896-901 (2016). PubMed: 27621469
- Jimenez-Blasco D et al. Astrocyte NMDA receptors' activity sustains neuronal survival through a Cdk5-Nrf2 pathway. Cell Death Differ 22:1877-89 (2015). WB ; Rat . PubMed: 25909891
- Zhang T et al. G-protein-coupled receptors regulate autophagy by ZBTB16-mediated ubiquitination and proteasomal degradation of Atg14L. Elife 4:e06734 (2015). Human . PubMed: 25821988
- Manjarrez JR et al. Hsp90-dependent assembly of the DBC2/RhoBTB2-Cullin3 E3-ligase complex. PLoS One 9:e90054 (2014). WB ; Human . PubMed: 24608665
