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  1. Link

    cullin-3cul-3-antibody-epr3196y-ab75851.pdf

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RecombinantRabMAb

Recombinant Anti-Cullin 3/CUL-3 antibody [EPR3196Y] (ab75851)

  • Datasheet
Reviews (2) Submit a question References (10)

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Flow Cytometry (Intracellular) - Anti-Cullin 3/CUL-3 antibody [EPR3196Y] (ab75851)
  • Western blot - Anti-Cullin 3/CUL-3 antibody [EPR3196Y] (ab75851)
  • Anti-Cullin 3/CUL-3 antibody [EPR3196Y] (ab75851)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR3196Y] to Cullin 3/CUL-3
  • Suitable for: Flow Cyt (Intra), IP, WB
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Carrier Free

You may also be interested in

Secondary
Product image
Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)

View more associated products

Overview

  • Product name

    Anti-Cullin 3/CUL-3 antibody [EPR3196Y]
    See all Cullin 3/CUL-3 primary antibodies
  • Description

    Rabbit monoclonal [EPR3196Y] to Cullin 3/CUL-3
  • Host species

    Rabbit
  • Tested applications

    Suitable for: Flow Cyt (Intra), IP, WBmore details
    Unsuitable for: ICC or IHC-P
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • HeLa, SH-SY5Y, PC12 and 3T3 cell lysates.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer

    pH: 7.20
    Preservative: 0.05% Sodium azide
    Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR3196Y
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Cell Cycle
    • Cell Cycle Inhibitors
    • Other
    • Cell Biology
    • Apoptosis
    • Nucleus
    • Other
    • Cell Biology
    • Proteolysis / Ubiquitin
    • Proteasome / Ubiquitin
    • Ubiquitin E3 Enzymes
    • SCF Complex E3 Ligase
    • Cancer
    • Cell cycle
    • Cell cycle inhibitors
    • Other
    • Cancer
    • Cell Death
    • Apoptosis
    • Nucleus
    • Other
    • Neuroscience
    • Processes

Associated products

  • Alternative Versions

    • Anti-Cullin 3/CUL-3 antibody [EPR3196Y] - BSA and Azide free (ab239868)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Positive Controls

    • PC-12 nuclear extract lysate (ab14884)
  • Recombinant Protein

    • Recombinant Human Cullin 3/CUL-3 protein (ab152872)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab75851 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt (Intra)
1/30 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

IP
Use at an assay dependent concentration.
WB (1)
1/20000 - 1/100000. Predicted molecular weight: 89 kDa.
Notes
Flow Cyt (Intra)
1/30 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

IP
Use at an assay dependent concentration.
WB
1/20000 - 1/100000. Predicted molecular weight: 89 kDa.
Application notes
Is unsuitable for ICC or IHC-P.

Target

  • Function

    Core component of multiple cullin-RING-based BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complexes which mediate the ubiquitination and subsequent proteasomal degradation of target proteins. As a scaffold protein may contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. The E3 ubiquitin-protein ligase activity of the complex is dependent on the neddylation of the cullin subunit and is inhibited by the association of the deneddylated cullin subunit with TIP120A/CAND1 (By similarity). The functional specificity of the BCR complex depends on the BTB domain-containing protein as the susbstrate recognition component. BCR(SPOP) is involved in ubiquitination of BMI1/PCGF4, H2AFY and DAXX, and probably GLI2 or GLI3. BCR(KLHL9-KLHL13) controls the dynamic behavior of AURKB on mitotic chromosomes and thereby coordinates faithful mitotic progression and completion of cytokinesis. Involved in ubiquitination of cyclin E and of cyclin D1 (in vitro) thus involved in regulation of G1/S transition.
  • Tissue specificity

    Widely expressed.
  • Pathway

    Protein modification; protein ubiquitination.
  • Sequence similarities

    Belongs to the cullin family.
  • Post-translational
    modifications

    Neddylated. Attachment of NEDD8 is required for the E3 ubiquitin-protein ligase activity of the BCR complex. Deneddylated via its interaction with the COP9 signalosome (CSN) complex.
  • Cellular localization

    Nucleus. Golgi apparatus.
  • Target information above from: UniProt accession Q13618 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 8452 Human
    • Entrez Gene: 26554 Mouse
    • Entrez Gene: 301555 Rat
    • Omim: 603136 Human
    • SwissProt: Q13618 Human
    • SwissProt: Q9JLV5 Mouse
    • SwissProt: B5DF89 Rat
    • Unigene: 372286 Human
    • Unigene: 12665 Mouse
    • Unigene: 101949 Rat
    see all
  • Alternative names

    • CUL 3 antibody
    • Cul-3 antibody
    • cul3 antibody
    • CUL3_HUMAN antibody
    • Cullin 3 antibody
    • Cullin-3 antibody
    • Cullin3 antibody
    • KIAA0617 antibody
    • PHA2E antibody
    see all

Images

  • Flow Cytometry (Intracellular) - Anti-Cullin 3/CUL-3 antibody [EPR3196Y] (ab75851)
    Flow Cytometry (Intracellular) - Anti-Cullin 3/CUL-3 antibody [EPR3196Y] (ab75851)

    Overlay histogram showing HeLa cells stained with ab75851 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab75851, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

     

  • Western blot - Anti-Cullin 3/CUL-3 antibody [EPR3196Y] (ab75851)
    Western blot - Anti-Cullin 3/CUL-3 antibody [EPR3196Y] (ab75851)
    All lanes : Anti-Cullin 3/CUL-3 antibody [EPR3196Y] (ab75851) at 1/20000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : SH-SY5Y cell lysate
    Lane 3 : PC12 cell lysate
    Lane 4 : 3T3 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP-labeled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 89 kDa
    Observed band size: 82,90 kDa why is the actual band size different from the predicted?

  • Anti-Cullin 3/CUL-3 antibody [EPR3196Y] (ab75851)
    Anti-Cullin 3/CUL-3 antibody [EPR3196Y] (ab75851)

Protocols

  • Western blot protocols
  • Flow cytometry protocols

Click here to view the general protocols

Datasheets and documents

  • Datasheet download

    Download

References (10)

Publishing research using ab75851? Please let us know so that we can cite the reference in this datasheet.

ab75851 has been referenced in 10 publications.

  • Chen X  et al. Long non-coding RNA XIST inhibits osteoblast differentiation and promotes osteoporosis via Nrf2 hyperactivation by targeting CUL3. Int J Mol Med 48:N/A (2021). PubMed: 34036379
  • Lv W  et al. Snail promotes prostate cancer migration by facilitating SPOP ubiquitination and degradation. Biochem Biophys Res Commun 529:799-804 (2020). PubMed: 32736710
  • Füzesi-Levi MG  et al. CSNAP, the smallest CSN subunit, modulates proteostasis through cullin-RING ubiquitin ligases. Cell Death Differ N/A:N/A (2019). PubMed: 31367012
  • Zhou W  et al. Potent 5-Cyano-6-phenyl-pyrimidin-Based Derivatives Targeting DCN1-UBE2M Interaction. J Med Chem 62:5382-5403 (2019). PubMed: 31157974
  • Jang SM  et al. The replication initiation determinant protein (RepID) modulates replication by recruiting CUL4 to chromatin. Nat Commun 9:2782 (2018). PubMed: 30018425
  • Yang N  et al. Carnosic acid prevents dextran sulfate sodium-induced acute colitis associated with the regulation of the Keap1/Nrf2 pathway. Sci Rep 7:11036 (2017). PubMed: 28887507
  • Tan X  et al. PtdIns(4,5)P2 signaling regulates ATG14 and autophagy. Proc Natl Acad Sci U S A 113:10896-901 (2016). PubMed: 27621469
  • Jimenez-Blasco D  et al. Astrocyte NMDA receptors' activity sustains neuronal survival through a Cdk5-Nrf2 pathway. Cell Death Differ 22:1877-89 (2015). WB ; Rat . PubMed: 25909891
  • Zhang T  et al. G-protein-coupled receptors regulate autophagy by ZBTB16-mediated ubiquitination and proteasomal degradation of Atg14L. Elife 4:e06734 (2015). Human . PubMed: 25821988
  • Manjarrez JR  et al. Hsp90-dependent assembly of the DBC2/RhoBTB2-Cullin3 E3-ligase complex. PLoS One 9:e90054 (2014). WB ; Human . PubMed: 24608665

Customer reviews and Q&As

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1-2 of 2 Abreviews or Q&A

Immunocytochemistry/ Immunofluorescence abreview for Anti-Cullin 3/CUL-3 antibody [EPR3196Y]

Good
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa)
Permeabilization
No
Specification
HeLa
Fixative
Methanol
Read More

DR. Kirk Mcmanus

Verified customer

Submitted Nov 06 2017

Western blot abreview for Anti-Cullin 3/CUL-3 antibody [EPR3196Y]

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (THP-1)
Gel Running Conditions
Reduced Denaturing (5-20% gradient)
Loading amount
10 µg
Specification
THP-1
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Read More

Abcam user community

Verified customer

Submitted Nov 12 2010

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