Anti-Cullin 4A/CUL-4A antibody (ab137418)
Key features and details
- Rabbit polyclonal to Cullin 4A/CUL-4A
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Cullin 4A/CUL-4A antibody
See all Cullin 4A/CUL-4A primary antibodies -
Description
Rabbit polyclonal to Cullin 4A/CUL-4A -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat
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Immunogen
Recombinant fragment corresponding to Human Cullin 4A/CUL-4A aa 119-385.
Database link: Q13619 -
Positive control
- 293T, A431, H1299, HeLa, HepG2, MOLT4 and Raji whole cell lysates.
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General notes
This product was previously labelled as Cullin 4a, Cullin 4A/B
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab137418 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/3000. Predicted molecular weight: 88 kDa. |
Target
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Function
Core component of multiple cullin-RING-based E3 ubiquitin-protein ligase complexes which mediate the ubiquitination and subsequent proteasomal degradation of target proteins. As a scaffold protein may contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. The E3 ubiquitin-protein ligase activity of the complex is dependent on the neddylation of the cullin subunit and is inhibited by the association of the deneddylated cullin subunit with TIP120A/CAND1. The functional specificity of the E3 ubiquitin-protein ligase complex depends on the variable substrate recognition component. DCX(DET1-COP1) directs ubiquitination of JUN. DCX(DDB2) directs ubiquitination of XPC. In association with RBX1, DDB1 and DDB2 is required for histone H3 and histone H4 ubiquitination in response to ultraviolet and may be important for subsequent DNA repair. DCX(DTL) plays a role in PCNA-dependent polyubiquitination of CDT1 and MDM2-dependent ubiquitination of TP53 in response to radiation-induced DNA damage and during DNA replication. In association with DDB1 and SKP2 probably is involved in ubiquitination of CDKN1B/p27kip. Is involved in ubiquitination of HOXA9. -
Pathway
Protein modification; protein ubiquitination. -
Sequence similarities
Belongs to the cullin family. -
Post-translational
modificationsNeddylated. Deneddylated via its interaction with the COP9 signalosome (CSN) complex (By similarity). Deneddylated by Epstein-Barr virus BPLF1 leading to a S-phase-like environment that is required for efficient replication of the viral genome. - Information by UniProt
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Database links
- Entrez Gene: 8451 Human
- Entrez Gene: 99375 Mouse
- Entrez Gene: 361181 Rat
- Omim: 603137 Human
- SwissProt: Q13619 Human
- SwissProt: Q3TCH7 Mouse
- Unigene: 339735 Human
- Unigene: 212861 Mouse
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Alternative names
- 2810470J21Rik antibody
- AW495282 antibody
- CUL 4A antibody
see all
Images
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Western blot - Anti-Cullin 4A/CUL-4A antibody (ab137418)Anti-Cullin 4A/CUL-4A antibody (ab137418) at 1/1000 dilution + HepG2 whole cell lysate at 30 µg
Predicted band size: 88 kDa
7.5% SDS PAGE
References (0)
ab137418 has not yet been referenced specifically in any publications.
