Product nameAnti-Cullin 4A/CUL-4A antibody
See all Cullin 4A/CUL-4A primary antibodies
DescriptionRabbit polyclonal to Cullin 4A/CUL-4A
Tested applicationsSuitable for: IHC-P, ICC/IF, WB, IPmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rabbit, Horse, Chicken, Dog, Turkey, Chimpanzee, Rhesus monkey, Gorilla, Sea urchin, Platypus
Synthetic peptide corresponding to Human Cullin 4A/CUL-4A (C terminal).
Database link: (NP_001008895.1
- HeLa, 293T and NIH3T3 whole cell lysates.
This product was previously labelled as Cullin 4a, Cullin 4A/B
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab72548 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
|WB||1/1000 - 1/10000. Detects a band of approximately 88 kDa (predicted molecular weight: 88 kDa).|
|IP||Use at 2-5 µg/mg of lysate.|
FunctionCore component of multiple cullin-RING-based E3 ubiquitin-protein ligase complexes which mediate the ubiquitination and subsequent proteasomal degradation of target proteins. As a scaffold protein may contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. The E3 ubiquitin-protein ligase activity of the complex is dependent on the neddylation of the cullin subunit and is inhibited by the association of the deneddylated cullin subunit with TIP120A/CAND1. The functional specificity of the E3 ubiquitin-protein ligase complex depends on the variable substrate recognition component. DCX(DET1-COP1) directs ubiquitination of JUN. DCX(DDB2) directs ubiquitination of XPC. In association with RBX1, DDB1 and DDB2 is required for histone H3 and histone H4 ubiquitination in response to ultraviolet and may be important for subsequent DNA repair. DCX(DTL) plays a role in PCNA-dependent polyubiquitination of CDT1 and MDM2-dependent ubiquitination of TP53 in response to radiation-induced DNA damage and during DNA replication. In association with DDB1 and SKP2 probably is involved in ubiquitination of CDKN1B/p27kip. Is involved in ubiquitination of HOXA9.
PathwayProtein modification; protein ubiquitination.
Sequence similaritiesBelongs to the cullin family.
modificationsNeddylated. Deneddylated via its interaction with the COP9 signalosome (CSN) complex (By similarity). Deneddylated by Epstein-Barr virus BPLF1 leading to a S-phase-like environment that is required for efficient replication of the viral genome.
- Information by UniProt
- 2810470J21Rik antibody
- AW495282 antibody
- CUL 4A antibody
All lanes : Anti-Cullin 4A/CUL-4A antibody (ab72548) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Lane 5 : NIH3T3 whole cell lysate at 50 µg
Predicted band size: 88 kDa
Observed band size: 88 kDa
Additional bands at: 117 kDa. We are unsure as to the identity of these extra bands.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma (left) and mouse squamous cell carcinoma (right) tissues labelling Cullin 4A/CUL-4A with ab72548 at 1/1000 (1µg/ml). Detection: DAB.
ab72548 staining Cullin 4A/CUL-4A in human primary fibroblasts by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde and blocked with 2% BSA for 2 hours at room temperature. Samples were incubated with primary antibody (1/300 in PBS + 2% BSA) for 14 hours at 4°C. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG polyclonal (13200) was used as the secondary antibody.
Detection of Human Cullin 4A/CUL-4A by Immunoprecipitation from Whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded), using ab72548 at 3 µg/mg lysate for IP and at 1 µg/ml for subsequent Western blot detection.
IHC image of ab72548 staining in human normal cervix formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH9, epitope retrieval solution 2) for 20 mins. The section was then incubated with ab72548, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
This product has been referenced in:
- Yu R et al. miR-377 targets CUL4A and regulates metastatic capability in ovarian cancer. Int J Mol Med 41:3147-3156 (2018). Read more (PubMed: 29512715) »
- Li C et al. High Expressions of CUL4A and TP53 in Colorectal Cancer Predict Poor Survival. Cell Physiol Biochem 51:2829-2842 (2018). Read more (PubMed: 30562757) »