Western blot abreview for Anti-Cullin 4a antibody

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Abreviews
Application
Western blot
Sample
Human Cell lysate - whole cell (HeLa cells)
Loading amount
20 µg
Specification
HeLa cells
Gel Running Conditions
Reduced Denaturing (10% Tris-Gly gel)
Blocking step
LI-COR® Odyssey® Blocking Buffer as blocking agent for 45 minute(s) · Concentration: 50% · Temperature: RT°C

Other product details

Dilution
1/5000
Incubation time
14 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: Odyssey® Blocker 1:1 in PBS + 0.1% Tween

Secondary antibody

Name
Non-Abcam antibody was used: AlexaFluor 680 goat anti-rabbit
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 680
Dilution
1/10000

Detection

Detection method
Odyssey® scanner
Exposure
2 minute(s) and 0 second(s)
Bands
Specific: 86 kDa Non-specific: 52 kDa
Positive control
Whole cell extract
Negative control
-

Additional data

Additional Notes
Picture legend.
1,2 - HeLa Whole Cell extract (20 & 40 µg, respectively)
Abcam response
Reducing antibody concentration or incubation time might improve the background.

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Submitted Jan 13 2011

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