Western blot abreview for Anti-Cullin 4a antibody

Excellent
Abreviews
Application
Western blot
Sample
Mouse Cell lysate - whole cell (Mouse embryonic fibroblasts)
Loading amount
20 µg
Specification
Mouse embryonic fibroblasts
Gel Running Conditions
Reduced Denaturing (10% Tris-Gly gel)
Blocking step
LI-COR® Odyssey® Blocking Buffer as blocking agent for 45 minute(s) · Concentration: 50% · Temperature: RT°C

Other product details

Dilution
1/5000
Incubation time
2 hour(s) and 0 minute(s) · Diluent: 50mMTris,pH8.0,50mMKCl,1mMEDTA,5%glycero

Secondary antibody

Name
Non-Abcam antibody was used: IR Dye 800 goat anti-rabbit
Host species: Goat
Clonality: Polyclonal
Conjugation: IRDye® 800CW
Dilution
1/10000

Detection

Detection method
Odyssey® scanner
Exposure
2 minute(s) and 0 second(s)
Bands
Specific: 88 kDa
Positive control
Whole Cell Extract
Negative control
-

Additional data

Additional Notes
Picture legend
Mouse embryonic fibroblasts Whole Cell Extract (20 µg)

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Submitted Nov 23 2011

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