RecombinantRabMAb

Recombinant Anti-Cullin 4A/CUL-4A antibody [EPR3198] (ab92554)

Reviews (1)Q&A (1)References (7)
Western blot - Anti-Cullin 4A/CUL-4A antibody [EPR3198] (ab92554)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cullin 4A/CUL-4A antibody [EPR3198] (ab92554)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cullin 4A/CUL-4A antibody [EPR3198] (ab92554)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cullin 4A/CUL-4A antibody [EPR3198] (ab92554)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cullin 4A/CUL-4A antibody [EPR3198] (ab92554)
  • Anti-Cullin 4A/CUL-4A antibody [EPR3198] (ab92554)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR3198] to Cullin 4A/CUL-4A
  • Suitable for: WB, IP, IHC-P
  • Reacts with: Human

Overview

  • Product name

    Anti-Cullin 4A/CUL-4A antibody [EPR3198]
    See all Cullin 4A/CUL-4A primary antibodies

  • Description

    Rabbit monoclonal [EPR3198] to Cullin 4A/CUL-4A

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IP, IHC-Pmore details
    Unsuitable for: Flow Cyt or ICC

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide within Human Cullin 4A/CUL-4A aa 700-800 (C terminal). The exact sequence is proprietary.
    Database link: Q13619

  • Positive control

    • WB: HeLa, A375 and 293T cell lysates. IHC-P: Human breast carcinoma tissue.

  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product was previously labelled as Cullin 4a, Cullin 4A/B.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.

  • Storage buffer

    pH: 7.20
    Preservative: 0.05% Sodium azide
    Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant
  • Concentration information loading...

  • Purity

    Tissue culture supernatant

  • Clonality

    Monoclonal

  • Clone number

    EPR3198

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab92554 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/20000 - 1/100000. Predicted molecular weight: 88 kDa.
IP 1/50.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for Flow Cyt or ICC.

    • Target

    • Function

      Core component of multiple cullin-RING-based E3 ubiquitin-protein ligase complexes which mediate the ubiquitination and subsequent proteasomal degradation of target proteins. As a scaffold protein may contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. The E3 ubiquitin-protein ligase activity of the complex is dependent on the neddylation of the cullin subunit and is inhibited by the association of the deneddylated cullin subunit with TIP120A/CAND1. The functional specificity of the E3 ubiquitin-protein ligase complex depends on the variable substrate recognition component. DCX(DET1-COP1) directs ubiquitination of JUN. DCX(DDB2) directs ubiquitination of XPC. In association with RBX1, DDB1 and DDB2 is required for histone H3 and histone H4 ubiquitination in response to ultraviolet and may be important for subsequent DNA repair. DCX(DTL) plays a role in PCNA-dependent polyubiquitination of CDT1 and MDM2-dependent ubiquitination of TP53 in response to radiation-induced DNA damage and during DNA replication. In association with DDB1 and SKP2 probably is involved in ubiquitination of CDKN1B/p27kip. Is involved in ubiquitination of HOXA9.

    • Pathway

      Protein modification; protein ubiquitination.

    • Sequence similarities

      Belongs to the cullin family.

    • Post-translational
      modifications

      Neddylated. Deneddylated via its interaction with the COP9 signalosome (CSN) complex (By similarity). Deneddylated by Epstein-Barr virus BPLF1 leading to a S-phase-like environment that is required for efficient replication of the viral genome.
    • Information by UniProt

    • Database links

      • Alternative names

        • 2810470J21Rik antibody
        • AW495282 antibody
        • CUL 4A antibody
        • CUL-4A antibody
        • Cul4a antibody
        • Cul4a protein antibody
        • CUL4A_HUMAN antibody
        • Cullin-4A antibody
        • MGC36573 antibody
        • MGC64071 antibody
        see all

      Images

      • Western blot - Anti-Cullin 4A/CUL-4A antibody [EPR3198] (ab92554)
        Western blot - Anti-Cullin 4A/CUL-4A antibody [EPR3198] (ab92554)
        All lanes : Anti-Cullin 4A/CUL-4A antibody [EPR3198] (ab92554) at 1/20000 dilution

        Lane 1 : Hela cell lysate
        Lane 2 : A375 cell lysate
        Lane 3 : 293T cell lysate

        Lysates/proteins at 10 µg per lane.

        Secondary
        All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

        Predicted band size: 88 kDa

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cullin 4A/CUL-4A antibody [EPR3198] (ab92554)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cullin 4A/CUL-4A antibody [EPR3198] (ab92554)

        ab92554 at 1/100 dilution staining Cullin 4A/CUL-4A in Human breast carcinoma by Immunohistochemistry Formalin-fixed, Paraffin-embedded tissue. Detection: DAB staining.

        Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cullin 4A/CUL-4A antibody [EPR3198] (ab92554)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cullin 4A/CUL-4A antibody [EPR3198] (ab92554)

        ab92554 showing positive staining in Hepatocellular carcinoma tissue.

        Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cullin 4A/CUL-4A antibody [EPR3198] (ab92554)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cullin 4A/CUL-4A antibody [EPR3198] (ab92554)

        ab92554 showing positive staining in Colonic adenocarcinoma tissue.

        Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cullin 4A/CUL-4A antibody [EPR3198] (ab92554)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cullin 4A/CUL-4A antibody [EPR3198] (ab92554)

        ab92554 showing positive staining in Ovarian carcinoma tissue.

        Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.

      • Anti-Cullin 4A/CUL-4A antibody [EPR3198] (ab92554)
        Anti-Cullin 4A/CUL-4A antibody [EPR3198] (ab92554)

      References (7)

      Publishing research using ab92554? Please let us know so that we can cite the reference in this datasheet.

      ab92554 has been referenced in 7 publications.

      • Yang C  et al. Small molecule NSC1892 targets the CUL4A/4B-DDB1 interactions and causes impairment of CRL4DCAF4 E3 ligases to inhibit colorectal cancer cell growth. Int J Biol Sci 16:1059-1070 (2020). PubMed: 32140073
      • Ji L  et al. Increased Expression of Serine Hydroxymethyltransferase 2 (SHMT2) is a Negative Prognostic Marker in Patients with Hepatocellular Carcinoma and is Associated with Proliferation of HepG2 Cells. Med Sci Monit 25:5823-5832 (2019). PubMed: 31379360
      • Jang SM  et al. The replication initiation determinant protein (RepID) modulates replication by recruiting CUL4 to chromatin. Nat Commun 9:2782 (2018). PubMed: 30018425
      • Zhu QN  et al. LncRNA H19 is a major mediator of doxorubicin chemoresistance in breast cancer cells through a cullin4A-MDR1 pathway. Oncotarget 8:91990-92003 (2017). PubMed: 29190892
      • Hung MS  et al. Knockdown of cullin 4A inhibits growth and increases chemosensitivity in lung cancer cells. J Cell Mol Med 20:1295-306 (2016). WB ; Human . PubMed: 26969027
      • Ikoma-Seki K  et al. Role of LRP1 and ERK and cAMP Signaling Pathways in Lactoferrin-Induced Lipolysis in Mature Rat Adipocytes. PLoS One 10:e0141378 (2015). WB ; Rat . PubMed: 26506094
      • Hofmann H  et al. Inhibition of CUL4A Neddylation causes a reversible block to SAMHD1-mediated restriction of HIV-1. J Virol 87:11741-50 (2013). WB ; Human . PubMed: 23986575

      Customer reviews and Q&As

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      1-2 of 2 Abreviews or Q&A

      Immunocytochemistry/ Immunofluorescence abreview for Anti-Cullin 4A/CUL-4A antibody [EPR3198]

       Average
      Abreviews
      abreview image
      Application
      Immunocytochemistry/ Immunofluorescence
      Sample
      Human Cell (HeLa)
      Permeabilization
      Yes - 0.5% Triton-X100 in PBS
      Specification
      HeLa
      Fixative
      Paraformaldehyde
      Read More

      Dr. Kirk Mcmanus

      Verified customer

      Submitted Aug 20 2014

      Question

      Dear Abcam, I'd like to know the exact amminoacid sequence of CUL4A recognized by this antibody. In particular, for my experiments I need a monoclonal antibody against the N-terminal region of CUL4A. Thank you for the support.

      Read More
      Answer

      Thank you for contacting Abcam.
      The immunogen peptide for ab92554 maps between amino acids *****and ****** near the C-terminus of human CUL4A (UniProt: Q13619).

      If there is anything else I can help you with, please let me know.

      Read More

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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