Overview

  • Product name

    Anti-Cullin 4A/CUL-4A antibody - N-terminal
    See all Cullin 4A/CUL-4A primary antibodies
  • Description

    Rabbit polyclonal to Cullin 4A/CUL-4A - N-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human Cullin 4A/CUL-4A (N terminal). The exact sequence is proprietary. (Carrier-protein conjugated).
    Database link: Q13619

  • Positive control

    • WB; HEK-293T, A431, HeLa, HepG2 and A-375 whole cell extracts.
  • General notes

     This product was previously labelled as Cullin 4a, Cullin 4A/B

     

Properties

Applications

Our Abpromise guarantee covers the use of ab227615 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 88 kDa.

Target

  • Function

    Core component of multiple cullin-RING-based E3 ubiquitin-protein ligase complexes which mediate the ubiquitination and subsequent proteasomal degradation of target proteins. As a scaffold protein may contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. The E3 ubiquitin-protein ligase activity of the complex is dependent on the neddylation of the cullin subunit and is inhibited by the association of the deneddylated cullin subunit with TIP120A/CAND1. The functional specificity of the E3 ubiquitin-protein ligase complex depends on the variable substrate recognition component. DCX(DET1-COP1) directs ubiquitination of JUN. DCX(DDB2) directs ubiquitination of XPC. In association with RBX1, DDB1 and DDB2 is required for histone H3 and histone H4 ubiquitination in response to ultraviolet and may be important for subsequent DNA repair. DCX(DTL) plays a role in PCNA-dependent polyubiquitination of CDT1 and MDM2-dependent ubiquitination of TP53 in response to radiation-induced DNA damage and during DNA replication. In association with DDB1 and SKP2 probably is involved in ubiquitination of CDKN1B/p27kip. Is involved in ubiquitination of HOXA9.
  • Pathway

    Protein modification; protein ubiquitination.
  • Sequence similarities

    Belongs to the cullin family.
  • Post-translational
    modifications

    Neddylated. Deneddylated via its interaction with the COP9 signalosome (CSN) complex (By similarity). Deneddylated by Epstein-Barr virus BPLF1 leading to a S-phase-like environment that is required for efficient replication of the viral genome.
  • Information by UniProt
  • Database links

  • Alternative names

    • 2810470J21Rik antibody
    • AW495282 antibody
    • CUL 4A antibody
    • CUL-4A antibody
    • Cul4a antibody
    • Cul4a protein antibody
    • CUL4A_HUMAN antibody
    • Cullin-4A antibody
    • MGC36573 antibody
    • MGC64071 antibody
    see all

Images

  • All lanes : Anti-Cullin 4A/CUL-4A antibody - N-terminal (ab227615) at 1/1000 dilution

    Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
    Lane 2 : A431 (human epidermoid carcinoma cell line) whole cell lysate
    Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
    Lane 4 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
    Lane 5 : A-375 (human malignant melanoma cell line) whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 88 kDa



    7.5% SDS-PAGE gel.

References

ab227615 has not yet been referenced specifically in any publications.

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