Key features and details
- Rabbit polyclonal to Cullin 4A/CUL-4A - N-terminal
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Cullin 4A/CUL-4A antibody - N-terminal
See all Cullin 4A/CUL-4A primary antibodies
DescriptionRabbit polyclonal to Cullin 4A/CUL-4A - N-terminal
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
Synthetic peptide within Human Cullin 4A/CUL-4A (N terminal). The exact sequence is proprietary. (Carrier-protein conjugated).
Database link: Q13619
- WB; HEK-293T, A431, HeLa, HepG2 and A-375 whole cell extracts.
This product was previously labelled as Cullin 4a, Cullin 4A/B
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.025% Proclin 300
Constituents: PBS, 20% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab227615 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 88 kDa.|
FunctionCore component of multiple cullin-RING-based E3 ubiquitin-protein ligase complexes which mediate the ubiquitination and subsequent proteasomal degradation of target proteins. As a scaffold protein may contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. The E3 ubiquitin-protein ligase activity of the complex is dependent on the neddylation of the cullin subunit and is inhibited by the association of the deneddylated cullin subunit with TIP120A/CAND1. The functional specificity of the E3 ubiquitin-protein ligase complex depends on the variable substrate recognition component. DCX(DET1-COP1) directs ubiquitination of JUN. DCX(DDB2) directs ubiquitination of XPC. In association with RBX1, DDB1 and DDB2 is required for histone H3 and histone H4 ubiquitination in response to ultraviolet and may be important for subsequent DNA repair. DCX(DTL) plays a role in PCNA-dependent polyubiquitination of CDT1 and MDM2-dependent ubiquitination of TP53 in response to radiation-induced DNA damage and during DNA replication. In association with DDB1 and SKP2 probably is involved in ubiquitination of CDKN1B/p27kip. Is involved in ubiquitination of HOXA9.
PathwayProtein modification; protein ubiquitination.
Sequence similaritiesBelongs to the cullin family.
modificationsNeddylated. Deneddylated via its interaction with the COP9 signalosome (CSN) complex (By similarity). Deneddylated by Epstein-Barr virus BPLF1 leading to a S-phase-like environment that is required for efficient replication of the viral genome.
- Information by UniProt
- 2810470J21Rik antibody
- AW495282 antibody
- CUL 4A antibody
All lanes : Anti-Cullin 4A/CUL-4A antibody - N-terminal (ab227615) at 1/1000 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 2 : A431 (human epidermoid carcinoma cell line) whole cell lysate
Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 4 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
Lane 5 : A-375 (human malignant melanoma cell line) whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 88 kDa
7.5% SDS-PAGE gel.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab227615 has not yet been referenced specifically in any publications.