Overview

  • Product name

    Anti-Cullin 4B/CUL-4B antibody
    See all Cullin 4B/CUL-4B primary antibodies
  • Description

    Rabbit polyclonal to Cullin 4B/CUL-4B
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment within Human Cullin 4B/CUL-4B (internal sequence). The exact sequence is proprietary.
    Database link: Q13620

  • Positive control

    • WB: Neuro-2a, C8D30, NIH/3T3, RAW 264.7, C2C12, PC-12, Rat2, HEK-293T, A431, HeLa, HepG2 and A-375 whole cell lysate. IHC: Human esophagus carcinoma tissue. ICC/IF: HeLa cells.
  • General notes

    Protein previously labeled as Cullin 4B.

Properties

Applications

Our Abpromise guarantee covers the use of ab227724 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 104 kDa.
IHC-P 1/100 - 1/1000.
ICC/IF 1/100 - 1/1000.

Target

  • Function

    Core component of multiple cullin-RING-based E3 ubiquitin-protein ligase complexes which mediate the ubiquitination and subsequent proteasomal degradation of target proteins. The functional specificity of the E3 ubiquitin-protein ligase complex depends on the variable substrate recognition subunit. CUL4B may act within the complex as a scaffold protein, contributing to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. Plays a role as part of the E3 ubiquitin-protein ligase complex in polyubiquitination of CDT1, histone H2A, histone H3 and histone H4 in response to radiation-induced DNA damage. Targeted to UV damaged chromatin by DDB2 and may be important for DNA repair and DNA replication. Required for ubiquitination of cyclin E, and consequently, normal G1 cell cycle progression. Regulates the mammalian target-of-rapamycin (mTOR) pathway involved in control of cell growth, size and metabolism. Specific CUL4B regulation of the mTORC1-mediated pathway is dependent upon 26S proteasome function and requires interaction between CUL4B and MLST8.
  • Pathway

    Protein modification; protein ubiquitination.
  • Involvement in disease

    Defects in CUL4B are the cause of mental retardation syndromic X-linked Cabezas type (MRXC) [MIM:300354]; also known as mental retardation syndromic X-linked type 15. A syndromic form of X-linked mental retardation characterized by severe intellectual deficit associated with short stature, craniofacial dysmorphism, small testes, muscle wasting in lower legs, kyphosis, joint hyperextensibility, pes cavus, small feet, and abnormalities of the toes. Additional neurologic manifestations include speech delay and impairment, tremor, seizures, gait ataxia, hyperactivity and decreased attention span.
  • Sequence similarities

    Belongs to the cullin family.
  • Post-translational
    modifications

    Neddylated. Deneddylated via its interaction with the COP9 signalosome (CSN) complex.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • CUL 4B antibody
    • CUL-4B antibody
    • CUL4B antibody
    • CUL4B_HUMAN antibody
    • Cullin-4B antibody
    • DKFZp686F1470 antibody
    • KIAA0695 antibody
    • MRXHF2 antibody
    • MRXSC antibody
    • SFM2 antibody
    see all

Images

  • All lanes : Anti-Cullin 4B/CUL-4B antibody (ab227724) at 1/1000 dilution

    Lanes 1 & 4 : Neuro-2a (mouse neuroblastoma cell line) whole cell lysate
    Lane 2 : C8D30 whole cell lysate
    Lane 3 : NIH-3T3 (mouse embryo fibroblast cell line) whole cell lysate
    Lane 5 : C2C12 (mouse myoblast cell line) whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 104 kDa



    5% SDS-PAGE

  • HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for Cullin 4B/CUL-4B (green) using ab227724 at 1/1000 dilution in ICC/IF. Cells were fixed in 4% paraformaldehyde at RT for 15 minutes. Red - phalloidin cytoskeletal marker.

  • Paraffin-embedded human esophagus carcinoma tissue stained for Cullin 4B/CUL-4B using ab227724 at 1/1000 dilution in immunohistochemical analysis.

  • All lanes : Anti-Cullin 4B/CUL-4B antibody (ab227724) at 1/1000 dilution

    Lane 1 : PC-12 (rat adrenal gland pheochromocytoma cell line) whole cell lysate
    Lane 2 : Rat2 (rat fibroblast cell line) whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 104 kDa



    5% SDS-PAGE

  • All lanes : Anti-Cullin 4B/CUL-4B antibody (ab227724) at 1/1000 dilution

    Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
    Lane 2 : A431 (human epidermoid carcinoma cell line) whole cell lysate
    Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
    Lane 4 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 104 kDa



    5% SDS-PAGE

  • Anti-Cullin 4B/CUL-4B antibody (ab227724) at 1/1000 dilution + A-375 (human malignant melanoma cell line) whole cell lysate at 30 µg

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 104 kDa



    5% SDS-PAGE

References

ab227724 has not yet been referenced specifically in any publications.

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