Key features and details
- Rabbit polyclonal to Cullin 4B/CUL-4B
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-Cullin 4B/CUL-4B antibody
See all Cullin 4B/CUL-4B primary antibodies
DescriptionRabbit polyclonal to Cullin 4B/CUL-4B
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Mouse, Human
Synthetic peptide within Human Cullin 4B/CUL-4B aa 863-913. The exact sequence is proprietary. NP_003579.3
Database link: Q13620
- WB: HEK-293T, HeLa and jurkat whole cell lysate. IHC-P: Human prostate cancer tissue; Mouse teratoma tissue.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab245474 was affinity purified using an epitope specific to Cullin 4B/CUL-4B immobilized on solid support.
Our Abpromise guarantee covers the use of ab245474 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 104 kDa.|
|IHC-P||1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionCore component of multiple cullin-RING-based E3 ubiquitin-protein ligase complexes which mediate the ubiquitination and subsequent proteasomal degradation of target proteins. The functional specificity of the E3 ubiquitin-protein ligase complex depends on the variable substrate recognition subunit. CUL4B may act within the complex as a scaffold protein, contributing to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. Plays a role as part of the E3 ubiquitin-protein ligase complex in polyubiquitination of CDT1, histone H2A, histone H3 and histone H4 in response to radiation-induced DNA damage. Targeted to UV damaged chromatin by DDB2 and may be important for DNA repair and DNA replication. Required for ubiquitination of cyclin E, and consequently, normal G1 cell cycle progression. Regulates the mammalian target-of-rapamycin (mTOR) pathway involved in control of cell growth, size and metabolism. Specific CUL4B regulation of the mTORC1-mediated pathway is dependent upon 26S proteasome function and requires interaction between CUL4B and MLST8.
PathwayProtein modification; protein ubiquitination.
Involvement in diseaseDefects in CUL4B are the cause of mental retardation syndromic X-linked Cabezas type (MRXC) [MIM:300354]; also known as mental retardation syndromic X-linked type 15. A syndromic form of X-linked mental retardation characterized by severe intellectual deficit associated with short stature, craniofacial dysmorphism, small testes, muscle wasting in lower legs, kyphosis, joint hyperextensibility, pes cavus, small feet, and abnormalities of the toes. Additional neurologic manifestations include speech delay and impairment, tremor, seizures, gait ataxia, hyperactivity and decreased attention span.
Sequence similaritiesBelongs to the cullin family.
modificationsNeddylated. Deneddylated via its interaction with the COP9 signalosome (CSN) complex.
- Information by UniProt
- CUL 4B antibody
- CUL-4B antibody
- CUL4B antibody
Formalin-fixed, paraffin-embedded mouse teratoma tissue stained for Cullin 4B/CUL-4B using ab245474 at 1/1000 dilution in immunohistochemical analysis. DAB staining.
Formalin-fixed, paraffin-embedded Human prostate carcinoma tissue stained for Cullin 4B/CUL-4B using ab245474 at 1/1000 dilution in immunohistochemical analysis. DAB staining.
All lanes : Anti-Cullin 4B/CUL-4B antibody (ab245474) at 0.1 µg/ml
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 15 µg
Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
Lane 4 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 104 kDa
Exposure time: 3 minutes
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab245474 has not yet been referenced specifically in any publications.