Overview

  • Product name

    Anti-Cullin 5/CUL-5 antibody [EPR14725]
    See all Cullin 5/CUL-5 primary antibodies
  • Description

    Rabbit monoclonal [EPR14725] to Cullin 5/CUL-5
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment within Human Cullin 5/CUL-5 aa 150-300. The exact sequence is proprietary.
    Database link: Q93034

  • Positive control

    • WB: Hela, MOLT4 or T47-D cell lysates. Human mouse and Rat heart tissue lysates.
  • General notes

     

    Previously labelled as Cullin 5. 

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR14725
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab184177 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 80 kDa (predicted molecular weight: 90 kDa).

Target

  • Function

    Core component of multiple SCF-like ECS (Elongin-Cullin 2/5-SOCS-box protein) E3 ubiquitin-protein ligase complexes, which mediate the ubiquitination and subsequent proteasomal degradation of target proteins. As a scaffold protein may contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. The functional specificity of the E3 ubiquitin-protein ligase complex depends on the variable substrate recognition component. ECS(SOCS1) seems to direct ubiquitination of JAK2. Seems to be involved in proteosomal degradation of p53/TP53 stimulated by adenovirus E1B-55 kDa protein. May form a cell surface vasopressin receptor.
  • Pathway

    Protein modification; protein ubiquitination.
  • Sequence similarities

    Belongs to the cullin family.
  • Post-translational
    modifications

    Neddylated; which enhances the ubiquitination activity of SCF and prevents binding of the inhibitor CAND1. Deneddylated via its interaction with the COP9 signalosome (CSN).
  • Information by UniProt
  • Database links

  • Alternative names

    • CUL 5 antibody
    • CUL-5 antibody
    • CUL5 antibody
    • CUL5_HUMAN antibody
    • Cullin 5 antibody
    • Cullin-5 (vasopressin-activated calcium-mobilizing receptor-1) antibody
    • Cullin-5 antibody
    • Cullin5 antibody
    • VACM 1 antibody
    • VACM-1 antibody
    • VACM1 antibody
    • Vasopressin activated calcium mobilizing receptor 1 antibody
    • Vasopressin activated calcium mobilizing receptor antibody
    • Vasopressin-activated calcium-mobilizing receptor 1 antibody
    • Vasopressin-activated calcium-mobilizing receptor-1 antibody
    see all

Images

  • All lanes : Anti-Cullin 5/CUL-5 antibody [EPR14725] (ab184177) at 1/2000 dilution (Purified)

    Lane 1 : Human heart lysates
    Lane 2 : Mouse heart lysates
    Lane 3 : Rat heart lysates

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 90 kDa
    Observed band size: 90 kDa

  • All lanes : Anti-Cullin 5/CUL-5 antibody [EPR14725] (ab184177) at 1/10000 dilution

    Lane 1 : Hela cell lysate
    Lane 2 : MOLT4 cell lysate
    Lane 3 : T47-D cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Peroxidase conjugated Goat anti-Rabbit IgG (H+L) at 1/1000 dilution

    Predicted band size: 90 kDa
    Observed band size: 80 kDa
    why is the actual band size different from the predicted?

References

This product has been referenced in:

  • Sánchez-Sánchez SM  et al. Rare RELN variants affect Reelin-DAB1 signal transduction in autism spectrum disorder. Hum Mutat 39:1372-1383 (2018). Read more (PubMed: 29969175) »
  • Jang SM  et al. The replication initiation determinant protein (RepID) modulates replication by recruiting CUL4 to chromatin. Nat Commun 9:2782 (2018). Read more (PubMed: 30018425) »
See all 4 Publications for this product

Customer reviews and Q&As

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