Recombinant
RabMAb

Recombinant Anti-Cullin 5/CUL-5 antibody [EPR14725] - BSA and Azide free (ab240281)

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Overview

  • Product name

    Anti-Cullin 5/CUL-5 antibody [EPR14725] - BSA and Azide free
    See all Cullin 5/CUL-5 primary antibodies

  • Description

    Rabbit monoclonal [EPR14725] to Cullin 5/CUL-5 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WBmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Recombinant fragment within Human Cullin 5/CUL-5 aa 150-300. The exact sequence is proprietary.
    Database link: Q93034

  • Positive control

    • WB: Hela, MOLT4 or T47-D cell lysates. Human mouse and Rat heart tissue lysates.

  • General notes

    Ab240281 is the carrier-free version of ab184177. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab240281 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab240281 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Detects a band of approximately 80 kDa (predicted molecular weight: 90 kDa).

Target

  • Function

    Core component of multiple SCF-like ECS (Elongin-Cullin 2/5-SOCS-box protein) E3 ubiquitin-protein ligase complexes, which mediate the ubiquitination and subsequent proteasomal degradation of target proteins. As a scaffold protein may contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. The functional specificity of the E3 ubiquitin-protein ligase complex depends on the variable substrate recognition component. ECS(SOCS1) seems to direct ubiquitination of JAK2. Seems to be involved in proteosomal degradation of p53/TP53 stimulated by adenovirus E1B-55 kDa protein. May form a cell surface vasopressin receptor.

  • Pathway

    Protein modification; protein ubiquitination.

  • Sequence similarities

    Belongs to the cullin family.

  • Post-translational
    modifications

    Neddylated; which enhances the ubiquitination activity of SCF and prevents binding of the inhibitor CAND1. Deneddylated via its interaction with the COP9 signalosome (CSN).
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • CUL 5 antibody
    • CUL-5 antibody
    • CUL5 antibody
    • CUL5_HUMAN antibody
    • Cullin 5 antibody
    • Cullin-5 (vasopressin-activated calcium-mobilizing receptor-1) antibody
    • Cullin-5 antibody
    • Cullin5 antibody
    • VACM 1 antibody
    • VACM-1 antibody
    • VACM1 antibody
    • Vasopressin activated calcium mobilizing receptor 1 antibody
    • Vasopressin activated calcium mobilizing receptor antibody
    • Vasopressin-activated calcium-mobilizing receptor 1 antibody
    • Vasopressin-activated calcium-mobilizing receptor-1 antibody
    see all

Images

  • Western blot - Anti-Cullin 5/CUL-5 antibody [EPR14725] - BSA and Azide free (ab240281)
    Western blot - Anti-Cullin 5/CUL-5 antibody [EPR14725] - BSA and Azide free (ab240281)
    All lanes : Anti-Cullin 5/CUL-5 antibody [EPR14725] (ab184177) at 1/10000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : MOLT-4 cell lysate
    Lane 3 : T47-D cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Peroxidase conjugated Goat anti-Rabbit IgG (H+L) at 1/1000 dilution

    Predicted band size: 90 kDa



    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab184177).

References

ab240281 has not yet been referenced specifically in any publications.

Publishing research using ab240281? Please let us know so that we can cite the reference in this datasheet.

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