Overview

  • Product name

    Anti-Cullin 7/CUL-7 antibody
    See all Cullin 7/CUL-7 primary antibodies
  • Description

    Rabbit polyclonal to Cullin 7/CUL-7
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to Human Cullin 7/CUL-7 aa 1370-1679.

  • Positive control

    • H1299, HeLa, HepG2, Molt-4, Raji
  • General notes

    This product was previously labelled as CUL-7

     

Properties

Applications

Our Abpromise guarantee covers the use of ab96861 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 191 kDa.

Target

  • Function

    Component of a probable SCF-like E3 ubiquitin-protein ligase complex, which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Probably plays a role in the degradation of proteins involved in endothelial proliferation and/or differentiation (By similarity). Seems not to promote polyubiquitination and proteasomal degradation of TP53. In vitro, complexes of CUL7 with either CUL9 or FBXW8 or TP53 contain E3 ubiquitin-protein ligase activity.
  • Tissue specificity

    Highly expressed in fetal kidney and adult skeletal muscle. Also abundant in fetal brain, as well as in adult pancreas, kidney, placenta and heart. Detected in trophoblasts, lymphoblasts, osteoblasts, chondrocytes and skin fibroblasts.
  • Pathway

    Protein modification; protein ubiquitination.
  • Involvement in disease

    Defects in CUL7 are the cause of 3M syndrome type 1 (3M1) [MIM:273750]. An autosomal recessive disorder characterized by severe pre- and postnatal growth retardation, facial dysmorphism, large head circumference, and normal intelligence and endocrine function. Skeletal changes include long slender tubular bones and tall vertebral bodies.
  • Sequence similarities

    Belongs to the cullin family.
    Contains 1 DOC domain.
  • Cellular localization

    Cytoplasm.
  • Information by UniProt
  • Database links

  • Alternative names

    • CUL-7 antibody
    • CUL7 antibody
    • CUL7_HUMAN antibody
    • Cullin 7 antibody
    • Cullin-7 antibody
    • dJ20C7.5 antibody
    • KIAA0076 antibody
    see all

Images

  • Anti-Cullin 7/CUL-7 antibody (ab96861) at 1/1000 dilution + Molt-4 cell lysate at 30 µg

    Predicted band size: 191 kDa



    A 7.5% SDS-PAGE gel was used.

References

This product has been referenced in:

  • Jang SM  et al. The replication initiation determinant protein (RepID) modulates replication by recruiting CUL4 to chromatin. Nat Commun 9:2782 (2018). Read more (PubMed: 30018425) »
See 1 Publication for this product

Customer reviews and Q&As

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa)
Permeabilization
Yes - 0.5% Triton X-100
Specification
HeLa
Fixative
Paraformaldehyde

Dr. Kirk Mcmanus

Verified customer

Submitted Jan 02 2018

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